Restriction map for
pFN21AE2397
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AvaI AviII AvrII BbrPI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FauI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MluI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SwaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 2029 GACGTC GACGT^C
AccI 422 GTATAC GT^[AC][GT]AC
AccII 1560 CGCG CG^CG
AccIII 663 TCCGGA T^CCGGA
Alw44I 514 GTGCAC G^TGCAC
AocI 247 CCTCAGG CC^T[ACGT]AGG
ApaLI 514 GTGCAC G^TGCAC
AseI 964 ATTAAT AT^TAAT
AsnI 964 ATTAAT AT^TAAT
Asp700I 85 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2336 TGGCCA TGG^CCA
BamHI 51 GGATCC G^GATCC
BbeI 689 GGCGCC GGCGC^C
BciVI 2528 GGATAC GTATCC(6/5)
BclI 1772 TGATCA T^GATCA
BcnI 2054 CCGGG CC^[GC]GG
BglI 2017 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2592 GGTCTC GGTCTC(1/5)
BsaWI 663 TCCGGA [AT]^CCGG[AT]
BseAI 663 TCCGGA T^CCGGA
BseRI 1181 GAGGAG GAGGAG(10/8)
BsmBI 1571 GAGACG CGTCTC(1/5)
BsmI 1959 GAATGC GAATGC(1/-1)
BspEI 663 TCCGGA T^CCGGA
BsrFI 557 ACCGGC [GA]^CCGG[CT]
Bst1107I 422 GTATAC GTA^TAC
BstUI 1560 CGCG CG^CG
BstZ17I 422 GTATAC GTA^TAC
Bsu36I 247 CCTCAGG CC^T[ACGT]AGG
Cfr10I 557 ACCGGC [GA]^CCGG[CT]
DrdI 394 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 247 CCTCAGG CC^T[ACGT]AGG
EcoT22I 740 ATGCAT ATGCA^T
FbaI 1772 TGATCA T^GATCA
KasI 689 GGCGCC G^GCGCC
MfeI 2654 CAATTG C^AATTG
MluNI 2336 TGGCCA TGG^CCA
MroI 663 TCCGGA T^CCGGA
MscI 2336 TGGCCA TGG^CCA
MunI 2654 CAATTG C^AATTG
MvnI 1560 CGCG CG^CG
NarI 689 GGCGCC GG^CGCC
NciI 2054 CCGGG CC^[GC]GG
NdeI 2378 CATATG CA^TATG
NsiI 740 ATGCAT ATGCA^T
PshBI 964 ATTAAT AT^TAAT
SacI 2752 GAGCTC GAGCT^C
SapI 2209 GCTCTTC GCTCTTC(1/4)
ScaI 2143 AGTACT AGT^ACT
SexAI 210 ACCTGGT A^CC[AT]GGT
SfoI 689 GGCGCC GGC^GCC
SmlI 1906 CTCAAG C^T[CT][GA]AG
SpeI 1024 ACTAGT A^CTAGT
StuI 28 AGGCCT AGG^CCT
XmnI 85 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 803 AAATTT [GA]^AATT[CT]
2474 AAATTC
AlwNI 980 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2769 CAGTGTCTG
ApoI 803 AAATTT [GA]^AATT[CT]
2474 AAATTC
AspI 1633 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACAGTGTC
BanII 1411 GAGCCC G[GA]GC[CT]^C
2752 GAGCTC
BbsI 2688 GAAGAC GAAGAC(2/6)
937 GTCTTC
BbvCI 627 CCTCAGC CCTCAGC(-5/-2)
1806 GCTGAGG
BpuAI 2688 GAAGAC GAAGAC(2/6)
937 GTCTTC
BspMI 323 ACCTGC ACCTGC(4/8)
2349 GCAGGT
EcoNI 2729 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2890 CCTCTAGAAGG
HaeII 689 GGCGCC [GA]GCGC^[CT]
1598 AGCGCC
HgaI 712 GACGC GACGC(5/10)
1752 GACGC
NcoI 198 CCATGG C^CATGG
1304 CCATGG
PflFI 1633 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACAGTGTC
PpuMI 1325 AGGACCC [GA]G^G[AT]CC[CT]
2886 AGGACCT
PstI 604 CTGCAG CTGCA^G
2452 CTGCAG
SfcI 604 CTGCAG C^T[GA][CT]AG
2452 CTGCAG
SphI 327 GCATGC GCATG^C
1536 GCATGC
TaqI 849 TCGA T^CGA
1553 TCGA
TfiI 846 GATTC G^A[AT]TC
1315 GAATC
Tth111I 1633 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACAGTGTC
TthHB8I 849 TCGA T^CGA
1553 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BbvI BfaI
BmyI BpmI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SspI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XbaI
XcmI XhoII