################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaI BsmBI Bsp1407I BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII CpoI DraI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KpnI MaeII MfeI MluI MseI MunI NarI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 92 TCCGGA T^CCGGA AcyI 1859 GACGCC G[GA]^CG[CT]C AocI 404 CCTCAGG CC^T[ACGT]AGG Aor51HI 418 AGCGCT AGC^GCT Asp700I 705 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 148 CCCGAG C^[CT]CG[GA]G AviII 959 TGCGCA TGC^GCA BcnI 195 CCCGG CC^[GC]GG BglII 336 AGATCT A^GATCT BsaHI 1859 GACGCC G[GA]^CG[CT]C BseAI 92 TCCGGA T^CCGGA BsgI 808 CTGCAC GTGCAG(16/14) BsiEI 1848 CGGCCG CG[GA][CT]^CG BsiWI 51 CGTACG C^GTACG BsmAI 2236 GAGAC GTCTC(1/5) BsmI 610 GAATGC GAATGC(1/-1) BsoBI 148 CCCGAG C^[CT]CG[GA]G BspDI 2053 ATCGAT AT^CGAT BspEI 92 TCCGGA T^CCGGA BspMI 956 ACCTGC ACCTGC(4/8) BsrDI 1717 CATTGC GCAATG(2/0) Bsu36I 404 CCTCAGG CC^T[ACGT]AGG ClaI 2053 ATCGAT AT^CGAT DraIII 1192 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 838 GACTTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1848 CGGCCG C^GGCCG EclXI 1848 CGGCCG C^GGCCG Eco47III 418 AGCGCT AGC^GCT Eco52I 1848 CGGCCG C^GGCCG Eco81I 404 CCTCAGG CC^T[ACGT]AGG FspI 959 TGCGCA TGC^GCA HaeII 418 AGCGCT [GA]GCGC^[CT] Hin1I 1859 GACGCC G[GA]^CG[CT]C HincII 1060 GTTGAC GT[CT]^[GA]AC HindII 1060 GTTGAC GT[CT]^[GA]AC KspI 326 CCGCGG CCGC^GG MroI 92 TCCGGA T^CCGGA NciI 195 CCCGG CC^[GC]GG NheI 550 GCTAGC G^CTAGC SacII 326 CCGCGG CCGC^GG StuI 1787 AGGCCT AGG^CCT Tsp509I 1854 AATT ^AATT XmnI 705 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 1129 GTGCAC G^TGCAC 1921 GTGCAC ApaI 191 GGGCCC GGGCC^C 216 GGGCCC ApaLI 1129 GTGCAC G^TGCAC 1921 GTGCAC BanI 22 GGCACC G^G[CT][GA]CC 1205 GGTGCC BsaBI 259 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1434 GATAAAGATC BsaWI 92 TCCGGA [AT]^CCGG[AT] 1652 ACCGGA BsrBI 1965 CCGCTC CCGCTC(-3/-3) 379 GAGCGG BsrFI 1510 GCCGGC [GA]^CCGG[CT] 1757 GCCGGC Cfr10I 1510 GCCGGC [GA]^CCGG[CT] 1757 GCCGGC HgaI 1483 GACGC GACGC(5/10) 1859 GACGC MamI 259 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1434 GATAAAGATC NaeI 1510 GCCGGC GCC^GGC 1757 GCCGGC NcoI 18 CCATGG C^CATGG 503 CCATGG NgoMIV 1510 GCCGGC G^CCGGC 1757 GCCGGC PflMI 334 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1191 CCACCCAGTGG PshAI 2160 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2238 GACAGGAGTC PstI 907 CTGCAG CTGCA^G 1540 CTGCAG PvuII 367 CAGCTG CAG^CTG 942 CAGCTG SacI 427 GAGCTC GAGCT^C 971 GAGCTC SfaNI 2021 GCATC GCATC(5/9) 2228 GCATC Van91I 334 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1191 CCACCCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanII BbsI BbvCI BbvI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII