Restriction map for
pFN21AB6270
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaI BsmBI
Bsp1407I BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII CpoI DraI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KasI
KpnI MaeII MfeI MluI MseI
MunI NarI NdeI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 92 TCCGGA T^CCGGA
AcyI 1859 GACGCC G[GA]^CG[CT]C
AocI 404 CCTCAGG CC^T[ACGT]AGG
Aor51HI 418 AGCGCT AGC^GCT
Asp700I 705 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 148 CCCGAG C^[CT]CG[GA]G
AviII 959 TGCGCA TGC^GCA
BcnI 195 CCCGG CC^[GC]GG
BglII 336 AGATCT A^GATCT
BsaHI 1859 GACGCC G[GA]^CG[CT]C
BseAI 92 TCCGGA T^CCGGA
BsgI 808 CTGCAC GTGCAG(16/14)
BsiEI 1848 CGGCCG CG[GA][CT]^CG
BsiWI 51 CGTACG C^GTACG
BsmAI 2236 GAGAC GTCTC(1/5)
BsmI 610 GAATGC GAATGC(1/-1)
BsoBI 148 CCCGAG C^[CT]CG[GA]G
BspDI 2053 ATCGAT AT^CGAT
BspEI 92 TCCGGA T^CCGGA
BspMI 956 ACCTGC ACCTGC(4/8)
BsrDI 1717 CATTGC GCAATG(2/0)
Bsu36I 404 CCTCAGG CC^T[ACGT]AGG
ClaI 2053 ATCGAT AT^CGAT
DraIII 1192 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 838 GACTTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1848 CGGCCG C^GGCCG
EclXI 1848 CGGCCG C^GGCCG
Eco47III 418 AGCGCT AGC^GCT
Eco52I 1848 CGGCCG C^GGCCG
Eco81I 404 CCTCAGG CC^T[ACGT]AGG
FspI 959 TGCGCA TGC^GCA
HaeII 418 AGCGCT [GA]GCGC^[CT]
Hin1I 1859 GACGCC G[GA]^CG[CT]C
HincII 1060 GTTGAC GT[CT]^[GA]AC
HindII 1060 GTTGAC GT[CT]^[GA]AC
KspI 326 CCGCGG CCGC^GG
MroI 92 TCCGGA T^CCGGA
NciI 195 CCCGG CC^[GC]GG
NheI 550 GCTAGC G^CTAGC
SacII 326 CCGCGG CCGC^GG
StuI 1787 AGGCCT AGG^CCT
Tsp509I 1854 AATT ^AATT
XmnI 705 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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Alw44I 1129 GTGCAC G^TGCAC
1921 GTGCAC
ApaI 191 GGGCCC GGGCC^C
216 GGGCCC
ApaLI 1129 GTGCAC G^TGCAC
1921 GTGCAC
BanI 22 GGCACC G^G[CT][GA]CC
1205 GGTGCC
BsaBI 259 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1434 GATAAAGATC
BsaWI 92 TCCGGA [AT]^CCGG[AT]
1652 ACCGGA
BsrBI 1965 CCGCTC CCGCTC(-3/-3)
379 GAGCGG
BsrFI 1510 GCCGGC [GA]^CCGG[CT]
1757 GCCGGC
Cfr10I 1510 GCCGGC [GA]^CCGG[CT]
1757 GCCGGC
HgaI 1483 GACGC GACGC(5/10)
1859 GACGC
MamI 259 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1434 GATAAAGATC
NaeI 1510 GCCGGC GCC^GGC
1757 GCCGGC
NcoI 18 CCATGG C^CATGG
503 CCATGG
NgoMIV 1510 GCCGGC G^CCGGC
1757 GCCGGC
PflMI 334 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1191 CCACCCAGTGG
PshAI 2160 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2238 GACAGGAGTC
PstI 907 CTGCAG CTGCA^G
1540 CTGCAG
PvuII 367 CAGCTG CAG^CTG
942 CAGCTG
SacI 427 GAGCTC GAGCT^C
971 GAGCTC
SfaNI 2021 GCATC GCATC(5/9)
2228 GCATC
Van91I 334 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1191 CCACCCAGTGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanII
BbsI BbvCI BbvI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XhoII