Restriction map for
pFN21AE0600
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# NO CUT ENZYMES ( 29 enzymes) #
##################################
AatII Acc65I AscI Asp718I AviII
BbeI BsiWI BssHII Bst1107I BstZ17I
CpoI FseI FspI KasI KpnI
KspI MluI NarI NotI NruI
PacI PmeI PshAI RsrII SacII
SfiI SfoI SgrAI SwaI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AclI 246 AACGTT AA^CGTT
AcyI 4232 GGCGTC G[GA]^CG[CT]C
Alw44I 13804 GTGCAC G^TGCAC
ApaLI 13804 GTGCAC G^TGCAC
AseI 9636 ATTAAT AT^TAAT
AsnI 9636 ATTAAT AT^TAAT
AvrII 13638 CCTAGG C^CTAGG
BlnI 13638 CCTAGG C^CTAGG
BsaHI 4232 GGCGTC G[GA]^CG[CT]C
BstBI 6403 TTCGAA TT^CGAA
DraIII 252 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 13473 GATATC GAT^ATC
Hin1I 4232 GGCGTC G[GA]^CG[CT]C
NheI 5143 GCTAGC G^CTAGC
NspV 6403 TTCGAA TT^CGAA
PshBI 9636 ATTAAT AT^TAAT
Psp1406I 246 AACGTT AA^CGTT
PvuI 8097 CGATCG CGAT^CG
SbfI 5829 CCTGCAGG CCTGCA^GG
SfuI 6403 TTCGAA TT^CGAA
SnaBI 3488 TACGTA TAC^GTA
Sse8387I 5829 CCTGCAGG CCTGCA^GG
XbaI 1560 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 2021 TCCGGA T^CCGGA
6794 TCCGGA
AflII 4161 CTTAAG C^TTAAG
12639 CTTAAG
AgeI 1395 ACCGGT A^CCGGT
15182 ACCGGT
Aor51HI 3728 AGCGCT AGC^GCT
15787 AGCGCT
ApaI 7546 GGGCCC GGGCC^C
11066 GGGCCC
BciVI 13662 GGATAC GTATCC(6/5)
3815 GTATCC
BfrI 4161 CTTAAG C^TTAAG
12639 CTTAAG
BglI 4715 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7270 GCCTTCCTGGC
BseAI 2021 TCCGGA T^CCGGA
6794 TCCGGA
BsmI 4533 GAATGC GAATGC(1/-1)
7575 GAATGC
BspDI 5053 ATCGAT AT^CGAT
14935 ATCGAT
BspEI 2021 TCCGGA T^CCGGA
6794 TCCGGA
ClaI 5053 ATCGAT AT^CGAT
14935 ATCGAT
DrdI 12499 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
14815 GACTTTGATGTC
EagI 1258 CGGCCG C^GGCCG
7504 CGGCCG
EclXI 1258 CGGCCG C^GGCCG
7504 CGGCCG
Eco47III 3728 AGCGCT AGC^GCT
15787 AGCGCT
Eco52I 1258 CGGCCG C^GGCCG
7504 CGGCCG
HpaI 2854 GTTAAC GTT^AAC
14212 GTTAAC
MroI 2021 TCCGGA T^CCGGA
6794 TCCGGA
PaeR7I 3787 CTCGAG C^TCGAG
14867 CTCGAG
PinAI 1395 ACCGGT A^CCGGT
15182 ACCGGT
SalI 3591 GTCGAC G^TCGAC
9299 GTCGAC
SexAI 6638 ACCTGGT A^CC[AT]GGT
8170 ACCTGGT
SmaI 374 CCCGGG CCC^GGG
15413 CCCGGG
SpeI 456 ACTAGT A^CTAGT
10695 ACTAGT
SphI 4118 GCATGC GCATG^C
12423 GCATGC
XhoI 3787 CTCGAG C^TCGAG
14867 CTCGAG
XmaCI 374 CCCGGG C^CCGGG
15413 CCCGGG
XmaI 374 CCCGGG C^CCGGG
15413 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 176 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApoI Asp700I AspEI AspHI
AspI AvaI AvaII BalI BamHI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BclI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NaeI NciI NcoI NdeI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI