Restriction map for
pFN21AE1197
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AviII BalI BamHI BclI BglII
BlpI Bpu1102I BsaBI BsaI BsiWI
BsmBI BspDI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstZ17I CelII
Cfr10I ClaI DraI DrdI Eam1105I
EcoRV EcoT22I FbaI FseI FspI
HpaI KspI MamI MfeI MluI
MluNI MscI MunI NaeI NdeI
NgoMIV NheI NotI NsiI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I RcaI SacII SalI SapI
SbfI SfiI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1499 GGTACC G^GTACC
AcyI 151 GGCGCC G[GA]^CG[CT]C
AflII 519 CTTAAG C^TTAAG
AocI 1450 CCTCAGG CC^T[ACGT]AGG
Aor51HI 988 AGCGCT AGC^GCT
Asp718I 1499 GGTACC G^GTACC
AspI 1971 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 972 CCTAGG C^CTAGG
BbeI 151 GGCGCC GGCGC^C
BbvCI 1280 CCTCAGC CCTCAGC(-5/-2)
BciVI 604 GTATCC GTATCC(6/5)
BfrI 519 CTTAAG C^TTAAG
BlnI 972 CCTAGG C^CTAGG
BsaHI 151 GGCGCC G[GA]^CG[CT]C
BsmI 865 GAATGC GAATGC(1/-1)
BspLU11I 1717 ACATGT A^CATGT
BsrDI 2517 CATTGC GCAATG(2/0)
BstAPI 438 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2483 TTCGAA TT^CGAA
BstEII 1274 GGTCACC G^GT[ACGT]ACC
BstPI 1274 GGTCACC G^GT[ACGT]ACC
BstYI 2708 AGATCC [GA]^GATC[CT]
Bsu36I 1450 CCTCAGG CC^T[ACGT]AGG
CpoI 1116 CGGACCG CG^G[AT]CCG
DraIII 2647 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 37 CGGCCG C^GGCCG
EclXI 37 CGGCCG C^GGCCG
Eco47III 988 AGCGCT AGC^GCT
Eco52I 37 CGGCCG C^GGCCG
Eco81I 1450 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1274 GGTCACC G^GT[ACGT]ACC
EcoRI 2401 GAATTC G^AATTC
Hin1I 151 GGCGCC G[GA]^CG[CT]C
HincII 2263 GTCAAC GT[CT]^[GA]AC
HindII 2263 GTCAAC GT[CT]^[GA]AC
HindIII 1688 AAGCTT A^AGCTT
KasI 151 GGCGCC G^GCGCC
KpnI 1499 GGTACC GGTAC^C
MflI 2708 AGATCC [GA]^GATC[CT]
MslI 2688 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 151 GGCGCC GG^CGCC
NruI 1083 TCGCGA TCG^CGA
NspV 2483 TTCGAA TT^CGAA
PflFI 1971 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 2508 CGATCG CGAT^CG
RsrII 1116 CGGACCG CG^G[AT]CCG
ScaI 2207 AGTACT AGT^ACT
SexAI 320 ACCTGGT A^CC[AT]GGT
SfoI 151 GGCGCC GGC^GCC
SfuI 2483 TTCGAA TT^CGAA
SmaI 108 CCCGGG CCC^GGG
TfiI 72 GAATC G^A[AT]TC
Tth111I 1971 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 311 CCACAGGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2708 AGATCC [GA]^GATC[CT]
XmaCI 108 CCCGGG C^CCGGG
XmaI 108 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 28 GTCTAC GT^[AC][GT]AC
1026 GTCTAC
Asp700I 72 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
781 GAACAGCTTC
BbrPI 2134 CACGTG CAC^GTG
2179 CACGTG
BsaAI 2134 CACGTG [CT]AC^GT[GA]
2179 CACGTG
BseRI 59 GAGGAG GAGGAG(10/8)
1951 GAGGAG
BsiEI 37 CGGCCG CG[GA][CT]^CG
2508 CGATCG
Bsp1407I 2130 TGTACA T^GTACA
2333 TGTACA
BspMI 1666 ACCTGC ACCTGC(4/8)
2630 ACCTGC
BsrGI 2130 TGTACA T^GTACA
2333 TGTACA
EarI 1312 CTCTTC CTCTTC(1/4)
2428 CTCTTC
EcoNI 327 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
552 CCTGAGCGAGG
Ksp632I 1312 CTCTTC CTCTTC(1/4)
2428 CTCTTC
MseI 520 TTAA T^TAA
542 TTAA
NcoI 737 CCATGG C^CATGG
2237 CCATGG
PmaCI 2134 CACGTG CAC^GTG
2179 CACGTG
PmlI 2134 CACGTG CAC^GTG
2179 CACGTG
PpuMI 1226 GGGACCT [GA]G^G[AT]CC[CT]
1260 GGGACCC
PstI 169 CTGCAG CTGCA^G
992 CTGCAG
SacI 1002 GAGCTC GAGCT^C
1709 GAGCTC
SphI 2315 GCATGC GCATG^C
2521 GCATGC
SspBI 2130 TGTACA T^GTACA
2333 TGTACA
StuI 638 AGGCCT AGG^CCT
2153 AGGCCT
Tru9I 520 TTAA T^TAA
542 TTAA
XmnI 72 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
781 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BsaWI BseAI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MroI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I