################################## # NO CUT ENZYMES ( 223 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MnlI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 171 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 3 GGACC G^G[AT]CC BmyI 131 GTGCCC G[AGT]GC[ACT]^C Bsp1286I 131 GTGCCC G[AGT]GC[ACT]^C BspMI 40 ACCTGC ACCTGC(4/8) BssKI 135 CCAGG ^CC[ACGT]GG BstNI 135 CCAGG CC^[AT]GG CfoI 20 GCGC GCG^C Cfr13I 3 GGACC G^G[ACGT]CC EarI 90 GAAGAG CTCTTC(1/4) EcoRII 135 CCAGG ^CC[AT]GG HhaI 20 GCGC GCG^C HinP1I 20 GCGC G^CGC Ksp632I 90 GAAGAG CTCTTC(1/4) MboII 90 GAAGA GAAGA(8/7) MspA1I 171 CAGCTG C[AC]G^C[GT]G MvaI 135 CCAGG CC^[AT]GG NlaIII 114 CATG CATG^ PvuII 171 CAGCTG CAG^CTG SapI 90 GAAGAGC GCTCTTC(1/4) Sau96I 3 GGACC G^G[ACGT]CC ScrFI 135 CCAGG CC^[ACGT]GG ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### BsaJI 134 CCCAGG C^C[ACGT][ACGT]GG 135 CCAGGG BsrI 25 ACTGG ACTGG(1/-1) 46 ACTGG BstAPI 62 GCAAAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 77 GCAACTCCTGC SfaNI 143 GCATC GCATC(5/9) 157 GCATC TspRI 24 CACTG CA[GC]TG[ACGT][ACGT]^ 45 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI BbvI BsgI Fnu4HI ItaI MwoI NlaIV TseI