##################################
# NO CUT ENZYMES (  223 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       Cfr10I      ClaI        
CpoI        DdeI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeI        MaeII       MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MnlI        MroI        MscI        MseI        
MslI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        Sau3AI      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AlwNI         171  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII           3  GGACC                G^G[AT]CC
BmyI          131  GTGCCC               G[AGT]GC[ACT]^C
Bsp1286I      131  GTGCCC               G[AGT]GC[ACT]^C
BspMI          40  ACCTGC               ACCTGC(4/8)
BssKI         135  CCAGG                ^CC[ACGT]GG
BstNI         135  CCAGG                CC^[AT]GG
CfoI           20  GCGC                 GCG^C
Cfr13I          3  GGACC                G^G[ACGT]CC
EarI           90  GAAGAG               CTCTTC(1/4)
EcoRII        135  CCAGG                ^CC[AT]GG
HhaI           20  GCGC                 GCG^C
HinP1I         20  GCGC                 G^CGC
Ksp632I        90  GAAGAG               CTCTTC(1/4)
MboII          90  GAAGA                GAAGA(8/7)
MspA1I        171  CAGCTG               C[AC]G^C[GT]G
MvaI          135  CCAGG                CC^[AT]GG
NlaIII        114  CATG                 CATG^
PvuII         171  CAGCTG               CAG^CTG
SapI           90  GAAGAGC              GCTCTTC(1/4)
Sau96I          3  GGACC                G^G[ACGT]CC
ScrFI         135  CCAGG                CC^[ACGT]GG


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

BsaJI         134  CCCAGG               C^C[ACGT][ACGT]GG
              135  CCAGGG              
BsrI           25  ACTGG                ACTGG(1/-1)
               46  ACTGG               
BstAPI         62  GCAAAGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
               77  GCAACTCCTGC         
SfaNI         143  GCATC                GCATC(5/9)
              157  GCATC               
TspRI          24  CACTG                CA[GC]TG[ACGT][ACGT]^
               45  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        BbvI        BsgI        Fnu4HI      ItaI        
MwoI        NlaIV       TseI