##################################
# NO CUT ENZYMES (  232 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       HphI        ItaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MnlI        MroI        MslI        MspA1I      
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIII      NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TseI        
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AfaI          108  GTAC                 GT^AC
AluI          143  AGCT                 AG^CT
BalI           38  TGGCCA               TGG^CCA
BbsI            3  GTCTTC               GAAGAC(2/6)
BpuAI           3  GTCTTC               GAAGAC(2/6)
BsmAI         123  GAGAC                GTCTC(1/5)
BsmFI          62  GTCCC                GGGAC(10/14)
BssKI          36  CCTGG                ^CC[ACGT]GG
BstF5I         83  GGATG                GGATG(2/0)
BstNI          36  CCTGG                CC^[AT]GG
EaeI           38  TGGCCA               [CT]^GGCC[GA]
EcoRII         36  CCTGG                ^CC[AT]GG
FokI           83  GGATG                GGATG(9/13)
HaeIII         39  GGCC                 GG^CC
MluNI          38  TGGCCA               TGG^CCA
MscI           38  TGGCCA               TGG^CCA
MseI           26  TTAA                 T^TAA
MvaI           36  CCTGG                CC^[AT]GG
RsaI          108  GTAC                 GT^AC
ScrFI          36  CCTGG                CC^[ACGT]GG
Tru9I          26  TTAA                 T^TAA
TspRI          73  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

BsrI           91  ACTGG                ACTGG(1/-1)
               72  CCAGT               
HinfI          32  GATTC                G^A[ACGT]TC
               78  GATTC               
MboII         134  GAAGA                GAAGA(8/7)
                4  TCTTC               
TfiI           32  GATTC                G^A[AT]TC
               78  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################