##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HphI        ItaI        KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MscI        MslI        MspA1I      MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        TseI        Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII         17  TCCGGA               T^CCGGA
AcsI           75  GAATTT               [GA]^AATT[CT]
AfaI          129  GTAC                 GT^AC
AluI          114  AGCT                 AG^CT
AlwI          136  GGATC                GGATC(4/5)
ApaI           63  GGGCCC               GGGCC^C
ApoI           75  GAATTT               [GA]^AATT[CT]
AvaII          39  GGTCC                G^G[AT]CC
BanII          63  GGGCCC               G[GA]GC[CT]^C
BfaI          112  CTAG                 C^TAG
BmyI           63  GGGCCC               G[AGT]GC[ACT]^C
BsaWI          17  TCCGGA               [AT]^CCGG[AT]
BseAI          17  TCCGGA               T^CCGGA
BseRI          58  GAGGAG               GAGGAG(10/8)
BsiYI          51  CCACTATGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           51  CCACTATGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I       63  GGGCCC               G[AGT]GC[ACT]^C
BspEI          17  TCCGGA               T^CCGGA
BssKI          67  CCTGG                ^CC[ACGT]GG
BstF5I         15  CATCC                GGATG(2/0)
BstNI          67  CCTGG                CC^[AT]GG
BstYI         136  GGATCT               [GA]^GATC[CT]
DdeI          116  CTAAG                C^T[ACGT]AG
DpnII         137  GATC                 ^GATC
EcoRII         67  CCTGG                ^CC[AT]GG
FokI           15  CATCC                GGATG(9/13)
HapII          18  CCGG                 C^CGG
HpaII          18  CCGG                 C^CGG
MaeI          112  CTAG                 C^TAG
MaeIII        108  GTTAC                ^GT[ACGT]AC
MboI          137  GATC                 ^GATC
MboII          72  GAAGA                GAAGA(8/7)
MflI          136  GGATCT               [GA]^GATC[CT]
MroI           17  TCCGGA               T^CCGGA
MseI          182  TTAA                 T^TAA
MspI           18  CCGG                 C^CGG
MvaI           67  CCTGG                CC^[AT]GG
NdeII         137  GATC                 ^GATC
RsaI          129  GTAC                 GT^AC
Sau3AI        137  GATC                 ^GATC
ScrFI          67  CCTGG                CC^[ACGT]GG
SfaNI          14  GCATC                GCATC(5/9)
Tru9I         182  TTAA                 T^TAA
Tsp509I        76  AATT                 ^AATT
TspRI          89  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         136  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

BsaJI          66  CCCTGG               C^C[ACGT][ACGT]GG
               67  CCTGGG              
DraII          62  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               63  GGGCCCT             
EcoO109I       62  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               63  GGGCCCT             
HaeIII          7  GGCC                 GG^CC
               64  GGCC                
NlaIV          47  GGAGCC               GG[ACGT]^[ACGT]CC
               63  GGGCCC              


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

Cfr13I      MnlI        Sau96I