Restriction map for
pFN21AE6352
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV FbaI FseI HaeII
HpaI KasI KpnI Ksp632I MamI
MluI MluNI MroI MscI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 75 enzymes) #
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AccII 792 CGCG CG^CG
AcyI 4 GACGCC G[GA]^CG[CT]C
AlwI 406 GATCC GGATC(4/5)
AlwNI 1063 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1054 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 454 TGCGCA TGC^GCA
BanII 880 GAGCCC G[GA]GC[CT]^C
BbsI 1054 GAAGAC GAAGAC(2/6)
BbvI 48 GCTGC GCAGC(8/12)
BcnI 1023 CCGGG CC^[GC]GG
BfaI 190 CTAG C^TAG
BlpI 807 GCTTAGC GC^T[ACGT]AGC
BpmI 763 CTGGAG CTGGAG(16/14)
Bpu1102I 807 GCTTAGC GC^T[ACGT]AGC
BpuAI 1054 GAAGAC GAAGAC(2/6)
BsaAI 442 TACGTG [CT]AC^GT[GA]
BsaHI 4 GACGCC G[GA]^CG[CT]C
BsgI 694 GTGCAG GTGCAG(16/14)
BsmAI 463 GAGAC GTCTC(1/5)
BsmBI 463 GAGACG CGTCTC(1/5)
Bsp1407I 716 TGTACA T^GTACA
BspHI 1079 TCATGA T^CATGA
BspLU11I 527 ACATGT A^CATGT
BsrDI 923 GCAATG GCAATG(2/0)
BsrGI 716 TGTACA T^GTACA
BstEII 618 GGTCACC G^GT[ACGT]ACC
BstPI 618 GGTCACC G^GT[ACGT]ACC
BstUI 792 CGCG CG^CG
BstYI 405 AGATCC [GA]^GATC[CT]
CelII 807 GCTTAGC GC^T[ACGT]AGC
CfoI 455 GCGC GCG^C
DpnII 406 GATC ^GATC
DraIII 55 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 618 GGTCACC G^GT[ACGT]ACC
EcoT14I 797 CCATGG C^C[AT][AT]GG
EcoT22I 1099 ATGCAT ATGCA^T
FspI 454 TGCGCA TGC^GCA
HapII 1023 CCGG C^CGG
HgaI 4 GACGC GACGC(5/10)
HhaI 455 GCGC GCG^C
Hin1I 4 GACGCC G[GA]^CG[CT]C
HinP1I 455 GCGC G^CGC
HincII 22 GTCAAC GT[CT]^[GA]AC
HindII 22 GTCAAC GT[CT]^[GA]AC
HindIII 805 AAGCTT A^AGCTT
HpaII 1023 CCGG C^CGG
KspI 791 CCGCGG CCGC^GG
MaeI 190 CTAG C^TAG
MboI 406 GATC ^GATC
MfeI 1049 CAATTG C^AATTG
MflI 405 AGATCC [GA]^GATC[CT]
MslI 621 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 791 CCGCGG C[AC]G^C[GT]G
MspI 1023 CCGG C^CGG
MunI 1049 CAATTG C^AATTG
MvnI 792 CGCG CG^CG
NciI 1023 CCGGG CC^[GC]GG
NcoI 797 CCATGG C^CATGG
NdeII 406 GATC ^GATC
NheI 189 GCTAGC G^CTAGC
NsiI 1099 ATGCAT ATGCA^T
NspI 527 ACATGT [GA]CATG^[CT]
PleI 126 GAGTC GAGTC(4/5)
RcaI 1079 TCATGA T^CATGA
SacII 791 CCGCGG CCGC^GG
Sau3AI 406 GATC ^GATC
ScaI 513 AGTACT AGT^ACT
SmlI 854 CTCAAG C^T[CT][GA]AG
SspBI 716 TGTACA T^GTACA
SspI 904 AATATT AAT^ATT
StyI 797 CCATGG C^C[AT][AT]GG
TseI 48 GCTGC G^C[AT]GC
XcmI 594 CCAGAAGAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 405 AGATCC [GA]^GATC[CT]
XmnI 1054 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcsI 398 AAATTC [GA]^AATT[CT]
1087 AAATTT
AfaI 514 GTAC GT^AC
717 GTAC
AflIII 443 ACGTGT A^C[GA][CT]GT
527 ACATGT
ApoI 398 AAATTC [GA]^AATT[CT]
1087 AAATTT
AvaII 37 GGTCC G^G[AT]CC
678 GGTCC
BanI 589 GGTGCC G^G[CT][GA]CC
787 GGTGCC
BspMI 644 ACCTGC ACCTGC(4/8)
15 GCAGGT
BstF5I 1020 CATCC GGATG(2/0)
558 GGATG
FauI 40 CCCGC CCCGC(4/6)
180 GCGGG
FokI 1020 CATCC GGATG(9/13)
558 GGATG
RsaI 514 GTAC GT^AC
717 GTAC
TfiI 171 GAATC G^A[AT]TC
860 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AluI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI BstXI Cac8I Cfr13I DdeI
DsaI EaeI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeII MaeIII
MboII MnlI MseI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI SfaNI
Tru9I Tsp45I Tsp509I TspRI