Restriction map for
pFN21AE1009
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I TspRI Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 420 GACGCC G[GA]^CG[CT]C
ApaI 521 GGGCCC GGGCC^C
AspHI 304 GAGCAC G[AT]GC[AT]^C
AspI 254 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 49 GAAGAC GAAGAC(2/6)
BbvCI 402 CCTCAGC CCTCAGC(-5/-2)
BfaI 349 CTAG C^TAG
BlpI 549 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 549 GCTGAGC GC^T[ACGT]AGC
BpuAI 49 GAAGAC GAAGAC(2/6)
BsaHI 420 GACGCC G[GA]^CG[CT]C
BsgI 565 GTGCAG GTGCAG(16/14)
BsiHKAI 304 GAGCAC G[AT]GC[AT]^C
BspMI 116 ACCTGC ACCTGC(4/8)
BsrI 648 CCAGT ACTGG(1/-1)
BssHII 448 GCGCGC G^CGCGC
BstF5I 87 GGATG GGATG(2/0)
CelII 549 GCTGAGC GC^T[ACGT]AGC
EarI 22 CTCTTC CTCTTC(1/4)
EcoT14I 557 CCAAGG C^C[AT][AT]GG
FokI 87 GGATG GGATG(9/13)
Hin1I 420 GACGCC G[GA]^CG[CT]C
HinfI 254 GACTC G^A[ACGT]TC
HphI 329 TCACC GGTGA(8/7)
Ksp632I 22 CTCTTC CTCTTC(1/4)
MaeI 349 CTAG C^TAG
MaeII 326 ACGT A^CGT
MslI 339 CACCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 315 CATG CATG^
NspI 314 GCATGT [GA]CATG^[CT]
PflFI 254 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 254 GACTC GAGTC(4/5)
PstI 514 CTGCAG CTGCA^G
SfcI 514 CTGCAG C^T[GA][CT]AG
StyI 557 CCAAGG C^C[AT][AT]GG
TaqI 302 TCGA T^CGA
Tsp45I 328 GTCAC ^GT[GC]AC
Tsp509I 416 AATT ^AATT
Tth111I 254 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 302 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 148 CCCGGG C^[CT]CG[GA]G
355 CCCGGG
BsoBI 148 CCCGGG C^[CT]CG[GA]G
355 CCCGGG
BsrBI 370 CCGCTC CCGCTC(-3/-3)
458 CCGCTC
BsrFI 331 ACCGGC [GA]^CCGG[CT]
602 GCCGGT
Cfr10I 331 ACCGGC [GA]^CCGG[CT]
602 GCCGGT
DdeI 403 CTCAG C^T[ACGT]AG
550 CTGAG
DsaI 167 CCGCGG C^C[GA][CT]GG
439 CCGCGG
HgaI 379 GACGC GACGC(5/10)
420 GACGC
KspI 167 CCGCGG CCGC^GG
439 CCGCGG
MaeIII 217 GTTAC ^GT[ACGT]AC
328 GTCAC
PpuMI 290 AGGACCT [GA]G^G[AT]CC[CT]
358 GGGTCCC
SacII 167 CCGCGG CCGC^GG
439 CCGCGG
SmaI 148 CCCGGG CCC^GGG
355 CCCGGG
XmaCI 148 CCCGGG C^CCGGG
355 CCCGGG
XmaI 148 CCCGGG C^CCGGG
355 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV Sau96I ScrFI TseI