Restriction map for
pFN21AE6729
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI ApaI AscI
AseI AsnI Asp718I AspEI AvrII
BbvCI BciVI BfaI BfrI BlnI
BlpI Bpu1102I BseAI BsiWI BspDI
BspEI BspLU11I Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII Eam1105I EcoO65I EcoRI
EcoRV FseI HincII HindII HindIII
HpaI KpnI MaeI MfeI MluI
MroI MunI NcoI NdeI NheI
NruI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI TfiI XbaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AspI 939 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2627 TGCGCA TGC^GCA
BamHI 1991 GGATCC G^GATCC
BbeI 283 GGCGCC GGCGC^C
BbrPI 2113 CACGTG CAC^GTG
BbsI 2424 GAAGAC GAAGAC(2/6)
BcgI 1703 GCATCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 851 TGATCA T^GATCA
BglII 665 AGATCT A^GATCT
BpuAI 2424 GAAGAC GAAGAC(2/6)
BsaBI 2625 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2974 GAGACC GGTCTC(1/5)
BsaWI 915 TCCGGT [AT]^CCGG[AT]
BsmBI 239 CGTCTC CGTCTC(1/5)
BsmI 732 GAATGC GAATGC(1/-1)
Bsp1407I 644 TGTACA T^GTACA
BsrFI 1933 GCCGGC [GA]^CCGG[CT]
BsrGI 644 TGTACA T^GTACA
BssHII 90 GCGCGC G^CGCGC
Cfr10I 1933 GCCGGC [GA]^CCGG[CT]
DrdI 2680 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2071 CGGCCG C^GGCCG
EclXI 2071 CGGCCG C^GGCCG
Eco52I 2071 CGGCCG C^GGCCG
FbaI 851 TGATCA T^GATCA
FspI 2627 TGCGCA TGC^GCA
KasI 283 GGCGCC G^GCGCC
KspI 2125 CCGCGG CCGC^GG
MamI 2625 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 3026 TTAA T^TAA
NaeI 1933 GCCGGC GCC^GGC
NarI 283 GGCGCC GG^CGCC
NgoMIV 1933 GCCGGC G^CCGGC
NotI 2070 GCGGCCGC GC^GGCCGC
PflFI 939 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2113 CACGTG CAC^GTG
PmlI 2113 CACGTG CAC^GTG
SacII 2125 CCGCGG CCGC^GG
ScaI 1835 AGTACT AGT^ACT
SfoI 283 GGCGCC GGC^GCC
SspBI 644 TGTACA T^GTACA
StuI 1998 AGGCCT AGG^CCT
Tru9I 3026 TTAA T^TAA
Tth111I 939 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcsI 586 GAATTT [GA]^AATT[CT]
823 AAATTT
Alw44I 2554 GTGCAC G^TGCAC
3019 GTGCAC
AocI 1803 CCTGAGG CC^T[ACGT]AGG
2877 CCTGAGG
Aor51HI 2011 AGCGCT AGC^GCT
2516 AGCGCT
ApaLI 2554 GTGCAC G^TGCAC
3019 GTGCAC
ApoI 586 GAATTT [GA]^AATT[CT]
823 AAATTT
Asp700I 2274 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2424 GAAGACCTTC
BanII 64 GGGCTC G[GA]GC[CT]^C
549 GGGCTC
BsaAI 1231 TACGTG [CT]AC^GT[GA]
2113 CACGTG
BsgI 2448 CTGCAC GTGCAG(16/14)
1792 GTGCAG
BspMI 374 ACCTGC ACCTGC(4/8)
442 GCAGGT
BsrDI 681 CATTGC GCAATG(2/0)
1596 CATTGC
BstAPI 1179 GCATGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2799 GCAGATGCTGC
BstXI 527 CCAACGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2334 CCAGTCCTGTGG
Bsu36I 1803 CCTGAGG CC^T[ACGT]AGG
2877 CCTGAGG
Eco47III 2011 AGCGCT AGC^GCT
2516 AGCGCT
Eco81I 1803 CCTGAGG CC^T[ACGT]AGG
2877 CCTGAGG
EcoT22I 921 ATGCAT ATGCA^T
2737 ATGCAT
NsiI 921 ATGCAT ATGCA^T
2737 ATGCAT
PaeR7I 58 CTCGAG C^TCGAG
3178 CTCGAG
PstI 385 CTGCAG CTGCA^G
2918 CTGCAG
SapI 211 GAAGAGC GCTCTTC(1/4)
834 GCTCTTC
SmaI 947 CCCGGG CCC^GGG
1105 CCCGGG
XhoI 58 CTCGAG C^TCGAG
3178 CTCGAG
XmaCI 947 CCCGGG C^CCGGG
1105 CCCGGG
XmaI 947 CCCGGG C^CCGGG
1105 CCCGGG
XmnI 2274 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2424 GAAGACCTTC
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII