Restriction map for
pFN21AE2239
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII AccI AccIII AciI AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MaeII
MamI MfeI MflI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 255 GGTACC G^GTACC
AccII 76 CGCG CG^CG
Alw44I 1009 GTGCAC G^TGCAC
AlwI 487 GATCC GGATC(4/5)
ApaLI 1009 GTGCAC G^TGCAC
AseI 31 ATTAAT AT^TAAT
AsnI 31 ATTAAT AT^TAAT
Asp700I 1120 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 255 GGTACC G^GTACC
AspHI 1009 GTGCAC G[AT]GC[AT]^C
BalI 965 TGGCCA TGG^CCA
BbsI 418 GAAGAC GAAGAC(2/6)
BcgI 909 GCACTTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 109 CCCGG CC^[GC]GG
BlpI 1049 GCTTAGC GC^T[ACGT]AGC
BpmI 788 CTCCAG CTGGAG(16/14)
Bpu1102I 1049 GCTTAGC GC^T[ACGT]AGC
BpuAI 418 GAAGAC GAAGAC(2/6)
BsaI 661 GAGACC GGTCTC(1/5)
BsaJI 1280 CCAAGG C^C[ACGT][ACGT]GG
BsgI 1259 CTGCAC GTGCAG(16/14)
BsiHKAI 1009 GTGCAC G[AT]GC[AT]^C
BsmFI 137 GGGAC GGGAC(10/14)
Bsp1407I 668 TGTACA T^GTACA
BsrGI 668 TGTACA T^GTACA
BstUI 76 CGCG CG^CG
BstXI 1280 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1049 GCTTAGC GC^T[ACGT]AGC
Cfr13I 87 GGCCC G^G[ACGT]CC
DraI 876 TTTAAA TTT^AAA
EaeI 965 TGGCCA [CT]^GGCC[GA]
EcoNI 423 CCTGTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1254 GAATTC G^AATTC
EcoT14I 1280 CCAAGG C^C[AT][AT]GG
HaeII 3 GGCGCT [GA]GCGC^[CT]
HapII 110 CCGG C^CGG
HpaII 110 CCGG C^CGG
KpnI 255 GGTACC GGTAC^C
MluNI 965 TGGCCA TGG^CCA
MscI 965 TGGCCA TGG^CCA
MspI 110 CCGG C^CGG
MvnI 76 CGCG CG^CG
NciI 109 CCCGG CC^[GC]GG
NspI 1224 GCATGC [GA]CATG^[CT]
PflMI 790 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 11 GAGTC GAGTC(4/5)
PshAI 421 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 31 ATTAAT AT^TAAT
SapI 547 GAAGAGC GCTCTTC(1/4)
Sau96I 87 GGCCC G^G[ACGT]CC
SmlI 541 CTTGAG C^T[CT][GA]AG
SpeI 1263 ACTAGT A^CTAGT
SphI 1224 GCATGC GCATG^C
SspBI 668 TGTACA T^GTACA
StyI 1280 CCAAGG C^C[AT][AT]GG
Van91I 790 CCAGAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 968 CCAAATACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1120 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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BanI 255 GGTACC G^G[CT][GA]CC
267 GGTGCC
BfaI 625 CTAG C^TAG
1264 CTAG
BstNI 17 CCAGG CC^[AT]GG
1146 CCTGG
EarI 1124 CTCTTC CTCTTC(1/4)
547 GAAGAG
EcoRII 17 CCAGG ^CC[AT]GG
1146 CCTGG
Ksp632I 1124 CTCTTC CTCTTC(1/4)
547 GAAGAG
MaeI 625 CTAG C^TAG
1264 CTAG
MspA1I 733 CAGCTG C[AC]G^C[GT]G
1249 CAGCTG
MvaI 17 CCAGG CC^[AT]GG
1146 CCTGG
PvuII 733 CAGCTG CAG^CTG
1249 CAGCTG
TfiI 864 GATTC G^A[AT]TC
1120 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
BbvI BmyI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I Cac8I
CfoI DdeI DpnII Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI ScrFI
SfaNI SfcI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I