Restriction map for
pFN21AE1520
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 145 GGTACC G^GTACC
AcsI 169 AAATTC [GA]^AATT[CT]
AfaI 146 GTAC GT^AC
AlwNI 12 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 169 AAATTC [GA]^AATT[CT]
Asp718I 145 GGTACC G^GTACC
AspHI 64 GTGCTC G[AT]GC[AT]^C
AviII 293 TGCGCA TGC^GCA
BanI 145 GGTACC G^G[CT][GA]CC
BanII 262 GAGCCC G[GA]GC[CT]^C
BsaWI 164 ACCGGA [AT]^CCGG[AT]
BsgI 79 GTGCAG GTGCAG(16/14)
BsiHKAI 64 GTGCTC G[AT]GC[AT]^C
BsmFI 95 GGGAC GGGAC(10/14)
BspMI 290 ACCTGC ACCTGC(4/8)
BsrI 254 CCAGT ACTGG(1/-1)
BstAPI 11 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 240 GGTCACC G^GT[ACGT]ACC
BstF5I 42 CATCC GGATG(2/0)
BstNI 149 CCAGG CC^[AT]GG
BstPI 240 GGTCACC G^GT[ACGT]ACC
BstYI 219 GGATCT [GA]^GATC[CT]
CfoI 294 GCGC GCG^C
Cfr13I 38 GGGCC G^G[ACGT]CC
DsaI 269 CCATGG C^C[GA][CT]GG
EcoO65I 240 GGTCACC G^GT[ACGT]ACC
EcoRII 149 CCAGG ^CC[AT]GG
FokI 42 CATCC GGATG(9/13)
FspI 293 TGCGCA TGC^GCA
HaeIII 39 GGCC GG^CC
HhaI 294 GCGC GCG^C
HinP1I 294 GCGC G^CGC
HinfI 331 GAGTC G^A[ACGT]TC
KpnI 145 GGTACC GGTAC^C
MflI 219 GGATCT [GA]^GATC[CT]
MnlI 143 GAGG CCTC(7/6)
MspA1I 15 CAGCTG C[AC]G^C[GT]G
MvaI 149 CCAGG CC^[AT]GG
NcoI 269 CCATGG C^CATGG
NlaIII 270 CATG CATG^
NlaIV 145 GGTACC GG[ACGT]^[ACGT]CC
PleI 331 GAGTC GAGTC(4/5)
PstI 18 CTGCAG CTGCA^G
PvuII 15 CAGCTG CAG^CTG
RsaI 146 GTAC GT^AC
SapI 123 GCTCTTC GCTCTTC(1/4)
Sau96I 38 GGGCC G^G[ACGT]CC
Tsp509I 170 AATT ^AATT
TspRI 255 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 50 CCACTCGCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 219 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AluI 16 AGCT AG^CT
83 AGCT
AlwI 152 GGATC GGATC(4/5)
219 GGATC
AvaI 91 CCCGGG C^[CT]CG[GA]G
138 CCCGGG
BmyI 64 GTGCTC G[AGT]GC[ACT]^C
262 GAGCCC
BsoBI 91 CCCGGG C^[CT]CG[GA]G
138 CCCGGG
Bsp1286I 64 GTGCTC G[AGT]GC[ACT]^C
262 GAGCCC
DpnII 153 GATC ^GATC
220 GATC
EarI 124 CTCTTC CTCTTC(1/4)
328 GAAGAG
EcoT14I 214 CCAAGG C^C[AT][AT]GG
269 CCATGG
Ksp632I 124 CTCTTC CTCTTC(1/4)
328 GAAGAG
MboI 153 GATC ^GATC
220 GATC
NdeII 153 GATC ^GATC
220 GATC
Sau3AI 153 GATC ^GATC
220 GATC
SfcI 18 CTGCAG C^T[GA][CT]AG
99 CTACAG
SmaI 91 CCCGGG CCC^GGG
138 CCCGGG
StyI 214 CCAAGG C^C[AT][AT]GG
269 CCATGG
XmaCI 91 CCCGGG C^CCGGG
138 CCCGGG
XmaI 91 CCCGGG C^CCGGG
138 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI BbvI BcnI BsaJI BsiYI
BslI BssKI Cac8I FauI Fnu4HI
HapII HpaII HphI ItaI MaeIII
MboII MspI MwoI NciI ScrFI
TseI Tsp45I