Restriction map for
pFN21AE3627
##################################
# NO CUT ENZYMES ( 177 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TfiI Tsp509I
TspRI XbaI XcmI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 163 GACGTC GACGT^C
AciI 48 CCGC CCGC(-3/-1)
AcyI 163 GACGTC G[GA]^CG[CT]C
AfaI 285 GTAC GT^AC
AlwI 254 GATCC GGATC(4/5)
AlwNI 267 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 145 GTGCTC G[AT]GC[AT]^C
AspI 163 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 45 GGACC G^G[AT]CC
BbsI 160 GAAGAC GAAGAC(2/6)
BcnI 42 CCCGG CC^[GC]GG
BpmI 264 CTCCAG CTGGAG(16/14)
BpuAI 160 GAAGAC GAAGAC(2/6)
BsaBI 248 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 163 GACGTC G[GA]^CG[CT]C
BsiHKAI 145 GTGCTC G[AT]GC[AT]^C
BsrI 266 CCAGT ACTGG(1/-1)
CfoI 276 GCGC GCG^C
CpoI 44 CGGACCG CG^G[AT]CCG
DpnII 254 GATC ^GATC
EaeI 2 TGGCCG [CT]^GGCC[GA]
HaeII 275 GGCGCT [GA]GCGC^[CT]
HapII 43 CCGG C^CGG
HgaI 153 GCGTC GACGC(5/10)
HhaI 276 GCGC GCG^C
Hin1I 163 GACGTC G[GA]^CG[CT]C
HinP1I 276 GCGC G^CGC
HinfI 262 GACTC G^A[ACGT]TC
HpaII 43 CCGG C^CGG
HphI 32 TCACC GGTGA(8/7)
MaeII 164 ACGT A^CGT
MamI 248 GATACCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 254 GATC ^GATC
MboII 160 GAAGA GAAGA(8/7)
MseI 109 TTAA T^TAA
MspI 43 CCGG C^CGG
NciI 42 CCCGG CC^[GC]GG
NdeII 254 GATC ^GATC
NspI 314 ACATGC [GA]CATG^[CT]
PaeR7I 322 CTCGAG C^TCGAG
PflFI 163 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 266 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 262 GACTC GAGTC(4/5)
RsaI 285 GTAC GT^AC
RsrII 44 CGGACCG CG^G[AT]CCG
Sau3AI 254 GATC ^GATC
SmlI 322 CTCGAG C^T[CT][GA]AG
StuI 178 AGGCCT AGG^CCT
Tru9I 109 TTAA T^TAA
Tsp45I 31 GTCAC ^GT[GC]AC
Tth111I 163 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 266 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 322 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AluI 230 AGCT AG^CT
326 AGCT
AvaI 19 CTCGGG C^[CT]CG[GA]G
322 CTCGAG
BmyI 9 GTGCCC G[AGT]GC[ACT]^C
145 GTGCTC
BsaJI 59 CCTGGG C^C[ACGT][ACGT]GG
171 CCTGGG
BsoBI 19 CTCGGG C^[CT]CG[GA]G
322 CTCGAG
Bsp1286I 9 GTGCCC G[AGT]GC[ACT]^C
145 GTGCTC
Cac8I 93 GCACGC GC[ACGT]^[ACGT]GC
209 GCCTGC
DdeI 258 CTCAG C^T[ACGT]AG
279 CTTAG
MwoI 93 GCACGCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
318 GCTTCTCGAGC
NlaIII 222 CATG CATG^
315 CATG
TaqI 116 TCGA T^CGA
323 TCGA
TthHB8I 116 TCGA T^CGA
323 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BbvI BsiYI BslI BssKI BstNI
Cfr13I EcoRII Fnu4HI HaeIII ItaI
MaeIII MnlI MvaI Sau96I ScrFI
TseI