Restriction map for
pFN21AE1535
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsiWI BspDI
BsrDI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SexAI SfuI
SgrAI SmlI SnaBI SpeI SspI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 356 TCCGGA T^CCGGA
AcyI 945 GGCGCC G[GA]^CG[CT]C
Asp700I 572 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 945 GGCGCC GGCGC^C
BfaI 34 CTAG C^TAG
BglII 89 AGATCT A^GATCT
BsaAI 852 CACGTA [CT]AC^GT[GA]
BsaHI 945 GGCGCC G[GA]^CG[CT]C
BseAI 356 TCCGGA T^CCGGA
BsiEI 1310 CGGCCG CG[GA][CT]^CG
BsmI 493 GAATGC GAATGC(1/-1)
Bsp1407I 1820 TGTACA T^GTACA
BspEI 356 TCCGGA T^CCGGA
BspLU11I 1904 ACATGT A^CATGT
BspMI 281 ACCTGC ACCTGC(4/8)
BsrBI 1624 GAGCGG CCGCTC(-3/-3)
BsrGI 1820 TGTACA T^GTACA
BssSI 1213 CACGAG CACGAG(-5/-1)
BstAPI 124 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1813 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1310 CGGCCG C^GGCCG
EclXI 1310 CGGCCG C^GGCCG
Eco52I 1310 CGGCCG C^GGCCG
Hin1I 945 GGCGCC G[GA]^CG[CT]C
HincII 157 GTCAAC GT[CT]^[GA]AC
HindII 157 GTCAAC GT[CT]^[GA]AC
HindIII 1480 AAGCTT A^AGCTT
KasI 945 GGCGCC G^GCGCC
MaeI 34 CTAG C^TAG
MroI 356 TCCGGA T^CCGGA
NarI 945 GGCGCC GG^CGCC
PpuMI 278 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 842 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 973 GAGCTC GAGCT^C
SapI 1465 GAAGAGC GCTCTTC(1/4)
ScaI 587 AGTACT AGT^ACT
SfoI 945 GGCGCC GGC^GCC
SmaI 1439 CCCGGG CCC^GGG
SspBI 1820 TGTACA T^GTACA
StuI 338 AGGCCT AGG^CCT
XmaCI 1439 CCCGGG C^CCGGG
XmaI 1439 CCCGGG C^CCGGG
XmnI 572 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcsI 138 GAATTC [GA]^AATT[CT]
430 GAATTC
AflIII 174 ACACGT A^C[GA][CT]GT
1904 ACATGT
ApoI 138 GAATTC [GA]^AATT[CT]
430 GAATTC
BsaWI 356 TCCGGA [AT]^CCGG[AT]
992 ACCGGA
BssHII 1668 GCGCGC G^CGCGC
1670 GCGCGC
DraII 278 AGGACCT [GA]G^G[ACGT]CC[CT]
2030 AGGCCCC
EcoO109I 278 AGGACCT [GA]G^G[ACGT]CC[CT]
2030 AGGCCCC
EcoRI 138 GAATTC G^AATTC
430 GAATTC
SbfI 768 CCTGCAGG CCTGCA^GG
1605 CCTGCAGG
SfiI 1938 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2022 GGCCTCCCAGGCC
Sse8387I 768 CCTGCAGG CCTGCA^GG
1605 CCTGCAGG
TfiI 205 GAATC G^A[AT]TC
2136 GAATC
Tsp45I 807 GTCAC ^GT[GC]AC
1246 GTCAC
Tsp509I 139 AATT ^AATT
431 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoNI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SphI StyI TaqI
TseI TspRI TthHB8I Van91I XcmI
XhoII