Restriction map for
pFN21AE1087
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI AscI AseI AsnI Asp700I
AspEI AspI AvrII BamHI BbrPI
BciVI BfrI BglII BlnI BsaBI
BseAI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI Eam1105I EcoO65I EcoRI EcoRV
HpaI MamI MfeI MluI MroI
MunI NheI NotI NruI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 1480 GAATTT [GA]^AATT[CT]
AflIII 1196 ACGTGT A^C[GA][CT]GT
AgeI 3557 ACCGGT A^CCGGT
Alw44I 1075 GTGCAC G^TGCAC
AocI 3564 CCTCAGG CC^T[ACGT]AGG
ApaLI 1075 GTGCAC G^TGCAC
ApoI 1480 GAATTT [GA]^AATT[CT]
AviII 3074 TGCGCA TGC^GCA
BbeI 31 GGCGCC GGCGC^C
BcgI 239 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2474 TGATCA T^GATCA
BsaAI 3209 CACGTA [CT]AC^GT[GA]
BsaI 75 GGTCTC GGTCTC(1/5)
BsiEI 2853 CGGCCG CG[GA][CT]^CG
BsmBI 2708 GAGACG CGTCTC(1/5)
Bsp1407I 1964 TGTACA T^GTACA
BsrGI 1964 TGTACA T^GTACA
BssSI 250 CACGAG CACGAG(-5/-1)
Bsu36I 3564 CCTCAGG CC^T[ACGT]AGG
DraIII 1258 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 592 GACGTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2853 CGGCCG C^GGCCG
EclXI 2853 CGGCCG C^GGCCG
Eco52I 2853 CGGCCG C^GGCCG
Eco81I 3564 CCTCAGG CC^T[ACGT]AGG
EcoT22I 2728 ATGCAT ATGCA^T
FbaI 2474 TGATCA T^GATCA
FseI 2016 GGCCGGCC GGCCGG^CC
FspI 3074 TGCGCA TGC^GCA
HindIII 3178 AAGCTT A^AGCTT
KasI 31 GGCGCC G^GCGCC
KspI 293 CCGCGG CCGC^GG
NarI 31 GGCGCC GG^CGCC
NdeI 2058 CATATG CA^TATG
NsiI 2728 ATGCAT ATGCA^T
PinAI 3557 ACCGGT A^CCGGT
SacII 293 CCGCGG CCGC^GG
ScaI 1181 AGTACT AGT^ACT
SfoI 31 GGCGCC GGC^GCC
SmaI 1758 CCCGGG CCC^GGG
SspBI 1964 TGTACA T^GTACA
StuI 2502 AGGCCT AGG^CCT
TfiI 3633 GATTC G^A[AT]TC
XmaCI 1758 CCCGGG C^CCGGG
XmaI 1758 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1715 GGTACC G^GTACC
1909 GGTACC
AcyI 31 GGCGCC G[GA]^CG[CT]C
2710 GACGCC
Aor51HI 2888 AGCGCT AGC^GCT
3648 AGCGCT
Asp718I 1715 GGTACC G^GTACC
1909 GGTACC
BbsI 1441 GAAGAC GAAGAC(2/6)
1582 GAAGAC
BfaI 1738 CTAG C^TAG
2149 CTAG
BlpI 909 GCTAAGC GC^T[ACGT]AGC
2884 GCTGAGC
Bpu1102I 909 GCTAAGC GC^T[ACGT]AGC
2884 GCTGAGC
BpuAI 1441 GAAGAC GAAGAC(2/6)
1582 GAAGAC
BsaHI 31 GGCGCC G[GA]^CG[CT]C
2710 GACGCC
BsaWI 1718 ACCGGA [AT]^CCGG[AT]
3557 ACCGGT
BsrBI 1234 CCGCTC CCGCTC(-3/-3)
574 GAGCGG
BstXI 2199 CCATGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2807 CCACTACTGTGG
BstYI 1392 GGATCT [GA]^GATC[CT]
3069 GGATCT
CelII 909 GCTAAGC GC^T[ACGT]AGC
2884 GCTGAGC
Eco47III 2888 AGCGCT AGC^GCT
3648 AGCGCT
Hin1I 31 GGCGCC G[GA]^CG[CT]C
2710 GACGCC
KpnI 1715 GGTACC GGTAC^C
1909 GGTACC
MaeI 1738 CTAG C^TAG
2149 CTAG
MflI 1392 GGATCT [GA]^GATC[CT]
3069 GGATCT
MseI 1484 TTAA T^TAA
2906 TTAA
PaeR7I 237 CTCGAG C^TCGAG
1118 CTCGAG
SbfI 2334 CCTGCAGG CCTGCA^GG
3063 CCTGCAGG
SexAI 3416 ACCTGGT A^CC[AT]GGT
3478 ACCAGGT
Sse8387I 2334 CCTGCAGG CCTGCA^GG
3063 CCTGCAGG
Tru9I 1484 TTAA T^TAA
2906 TTAA
XhoI 237 CTCGAG C^TCGAG
1118 CTCGAG
XhoII 1392 GGATCT [GA]^GATC[CT]
3069 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BglI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI