Restriction map for
pFN21AB3246
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccI AccIII AclI AflIII
AgeI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AviII
AvrII BanI BbeI BciVI BglII
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspLU11I
BsrBI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI KasI KpnI KspI
MamI MluI MroI NarI NcoI
NdeI NheI NotI NruI NsiI
PacI PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI Tth111I Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1941 GACGTC GACGT^C
AccII 9 CGCG CG^CG
Alw44I 1569 GTGCAC G^TGCAC
ApaLI 1569 GTGCAC G^TGCAC
BamHI 1797 GGATCC G^GATCC
BbrPI 1463 CACGTG CAC^GTG
BbsI 172 GAAGAC GAAGAC(2/6)
BbvCI 2763 GCTGAGG CCTCAGC(-5/-2)
BlpI 69 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 69 GCTGAGC GC^T[ACGT]AGC
BpuAI 172 GAAGAC GAAGAC(2/6)
BsaAI 1463 CACGTG [CT]AC^GT[GA]
BsaI 1759 GAGACC GGTCTC(1/5)
Bsp1407I 1492 TGTACA T^GTACA
BspMI 949 ACCTGC ACCTGC(4/8)
BsrGI 1492 TGTACA T^GTACA
BstBI 998 TTCGAA TT^CGAA
BstUI 9 CGCG CG^CG
CelII 69 GCTGAGC GC^T[ACGT]AGC
DraI 886 TTTAAA TTT^AAA
DraII 856 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 2540 CCGTGG C^C[GA][CT]GG
EarI 1712 GAAGAG CTCTTC(1/4)
EcoO109I 856 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1444 GAATTC G^AATTC
Ksp632I 1712 GAAGAG CTCTTC(1/4)
MfeI 305 CAATTG C^AATTG
MunI 305 CAATTG C^AATTG
MvnI 9 CGCG CG^CG
NaeI 1838 GCCGGC GCC^GGC
NgoMIV 1838 GCCGGC G^CCGGC
NspV 998 TTCGAA TT^CGAA
PaeR7I 2027 CTCGAG C^TCGAG
PmaCI 1463 CACGTG CAC^GTG
PmlI 1463 CACGTG CAC^GTG
PpuMI 856 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2575 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1591 AGTACT AGT^ACT
SexAI 2976 ACCTGGT A^CC[AT]GGT
SfuI 998 TTCGAA TT^CGAA
SspBI 1492 TGTACA T^GTACA
SspI 823 AATATT AAT^ATT
XbaI 1452 TCTAGA T^CTAGA
XhoI 2027 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflII 94 CTTAAG C^TTAAG
355 CTTAAG
AhdI 1468 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1936 GACGTGACGTC
AlwNI 1322 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2893 CAGTGGCTG
Asp700I 2062 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2268 GAAACAGTTC
AspEI 1468 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1936 GACGTGACGTC
AvaI 2027 CTCGAG C^[CT]CG[GA]G
2347 CCCGAG
BalI 1688 TGGCCA TGG^CCA
1895 TGGCCA
BcgI 1996 CGAGACGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2115 CGATGCTCCTGC
BclI 1865 TGATCA T^GATCA
2480 TGATCA
BfrI 94 CTTAAG C^TTAAG
355 CTTAAG
BglI 41 GCCACTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1907 GCCGCATCGGC
BpmI 677 CTGGAG CTGGAG(16/14)
1613 CTGGAG
BsmBI 1997 GAGACG CGTCTC(1/5)
2941 GAGACG
BsmFI 1874 GGGAC GGGAC(10/14)
580 GTCCC
BsoBI 2027 CTCGAG C^[CT]CG[GA]G
2347 CCCGAG
BsrFI 123 ACCGGC [GA]^CCGG[CT]
1838 GCCGGC
BssSI 590 CACGAG CACGAG(-5/-1)
2794 CTCGTG
BstYI 701 AGATCC [GA]^GATC[CT]
1797 GGATCC
Cfr10I 123 ACCGGC [GA]^CCGG[CT]
1838 GCCGGC
Eam1105I 1468 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1936 GACGTGACGTC
EcoNI 75 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2759 CCTTGCTGAGG
EcoT14I 1023 CCTTGG C^C[AT][AT]GG
2337 CCTTGG
FbaI 1865 TGATCA T^GATCA
2480 TGATCA
HaeII 2530 GGCGCT [GA]GCGC^[CT]
2694 GGCGCT
HindIII 91 AAGCTT A^AGCTT
1949 AAGCTT
HpaI 492 GTTAAC GTT^AAC
682 GTTAAC
MflI 701 AGATCC [GA]^GATC[CT]
1797 GGATCC
MluNI 1688 TGGCCA TGG^CCA
1895 TGGCCA
MscI 1688 TGGCCA TGG^CCA
1895 TGGCCA
PstI 935 CTGCAG CTGCA^G
1089 CTGCAG
SphI 1790 GCATGC GCATG^C
3025 GCATGC
StyI 1023 CCTTGG C^C[AT][AT]GG
2337 CCTTGG
XcmI 299 CCAATGCAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1575 CCACATTCGAGTTGG
XhoII 701 AGATCC [GA]^GATC[CT]
1797 GGATCC
XmnI 2062 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2268 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaII BanII
BbvI BcnI BfaI BmyI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I