Restriction map for
pFN21AE2563
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BamHI BbeI BciVI
BfrI BlnI BsaBI BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NarI NcoI NdeI NotI
NruI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 2029 GACGTC GACGT^C
AgeI 928 ACCGGT A^CCGGT
AocI 223 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1496 AGCGCT AGC^GCT
AvaI 137 CTCGAG C^[CT]CG[GA]G
BanI 964 GGTGCC G^G[CT][GA]CC
BbsI 1816 GAAGAC GAAGAC(2/6)
BbvCI 2962 GCTGAGG CCTCAGC(-5/-2)
BcgI 1712 GCAACTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1482 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1482 GCTGAGC GC^T[ACGT]AGC
BpuAI 1816 GAAGAC GAAGAC(2/6)
BsaWI 928 ACCGGT [AT]^CCGG[AT]
BsiEI 1435 CGGCCG CG[GA][CT]^CG
BsmI 2233 GAATGC GAATGC(1/-1)
BsoBI 137 CTCGAG C^[CT]CG[GA]G
Bsp1407I 292 TGTACA T^GTACA
BsrGI 292 TGTACA T^GTACA
BstEII 155 GGTCACC G^GT[ACGT]ACC
BstPI 155 GGTCACC G^GT[ACGT]ACC
Bsu36I 223 CCTGAGG CC^T[ACGT]AGG
CelII 1482 GCTGAGC GC^T[ACGT]AGC
DraIII 1286 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1435 CGGCCG C^GGCCG
EclXI 1435 CGGCCG C^GGCCG
Eco47III 1496 AGCGCT AGC^GCT
Eco52I 1435 CGGCCG C^GGCCG
Eco81I 223 CCTGAGG CC^T[ACGT]AGG
EcoNI 1719 CCTCGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 155 GGTCACC G^GT[ACGT]ACC
EcoRI 582 GAATTC G^AATTC
EcoRV 1700 GATATC GAT^ATC
EcoT22I 796 ATGCAT ATGCA^T
FseI 1436 GGCCGGCC GGCCGG^CC
NaeI 1437 GCCGGC GCC^GGC
NgoMIV 1437 GCCGGC G^CCGGC
NheI 2214 GCTAGC G^CTAGC
NsiI 796 ATGCAT ATGCA^T
PaeR7I 137 CTCGAG C^TCGAG
PinAI 928 ACCGGT A^CCGGT
ScaI 2804 AGTACT AGT^ACT
SmlI 137 CTCGAG C^T[CT][GA]AG
SspBI 292 TGTACA T^GTACA
XbaI 2613 TCTAGA T^CTAGA
XhoI 137 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 721 CGCG CG^CG
2551 CGCG
AlwI 2304 GATCC GGATC(4/5)
843 GGATC
ApaI 508 GGGCCC GGGCC^C
2967 GGGCCC
Asp700I 1407 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1851 GAAACAGTTC
BbrPI 2308 CACGTG CAC^GTG
2433 CACGTG
BclI 500 TGATCA T^GATCA
2225 TGATCA
BsaAI 2308 CACGTG [CT]AC^GT[GA]
2433 CACGTG
BsaI 1903 GAGACC GGTCTC(1/5)
104 GGTCTC
BseRI 140 GAGGAG GAGGAG(10/8)
2344 GAGGAG
BspHI 1185 TCATGA T^CATGA
1583 TCATGA
BsrDI 2958 CATTGC GCAATG(2/0)
908 GCAATG
BsrFI 928 ACCGGT [GA]^CCGG[CT]
1437 GCCGGC
BstUI 721 CGCG CG^CG
2551 CGCG
BstXI 1289 CCAGTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2066 CCATGAACCTGG
Cfr10I 928 ACCGGT [GA]^CCGG[CT]
1437 GCCGGC
DrdI 298 GACAACCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1615 GACAAGGCTGTC
DsaI 459 CCACGG C^C[GA][CT]GG
2744 CCGTGG
EarI 1587 GAAGAG CTCTTC(1/4)
2047 GAAGAG
FbaI 500 TGATCA T^GATCA
2225 TGATCA
HaeII 1496 AGCGCT [GA]GCGC^[CT]
2564 AGCGCC
HincII 1807 GTCAAC GT[CT]^[GA]AC
2035 GTCAAC
HindII 1807 GTCAAC GT[CT]^[GA]AC
2035 GTCAAC
Ksp632I 1587 GAAGAG CTCTTC(1/4)
2047 GAAGAG
MvnI 721 CGCG CG^CG
2551 CGCG
NspI 728 GCATGT [GA]CATG^[CT]
1119 GCATGT
PflMI 616 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2763 CCAGCTCCTGG
PmaCI 2308 CACGTG CAC^GTG
2433 CACGTG
PmlI 2308 CACGTG CAC^GTG
2433 CACGTG
RcaI 1185 TCATGA T^CATGA
1583 TCATGA
Van91I 616 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2763 CCAGCTCCTGG
XmnI 1407 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1851 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwNI ApoI AspHI AspI AvaII
BanII BbvI BcnI BfaI BglI
BglII BmyI BpmI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII