Restriction map for
pFN21AB9437
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AlwI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I ClaI
CpoI DpnII DraI DraIII DrdI
Eam1105I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MamI MboI
MfeI MflI MluI MroI MseI
MunI NdeI NdeII NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SapI Sau3AI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tth111I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 559 GTCTAC GT^[AC][GT]AC
AcsI 1460 GAATTC [GA]^AATT[CT]
Alw44I 508 GTGCAC G^TGCAC
AlwNI 171 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 35 AGCGCT AGC^GCT
ApaLI 508 GTGCAC G^TGCAC
ApoI 1460 GAATTC [GA]^AATT[CT]
AscI 490 GGCGCGCC GG^CGCGCC
Asp700I 33 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 812 TGGCCA TGG^CCA
BsaI 1063 GAGACC GGTCTC(1/5)
BsaWI 1755 ACCGGA [AT]^CCGG[AT]
BsmBI 774 CGTCTC CGTCTC(1/5)
BsrDI 1394 GCAATG GCAATG(2/0)
BstXI 572 CCACCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 35 AGCGCT AGC^GCT
EcoNI 1137 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1460 GAATTC G^AATTC
MluNI 812 TGGCCA TGG^CCA
MscI 812 TGGCCA TGG^CCA
PflMI 1355 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1675 GAGCTC GAGCT^C
SbfI 612 CCTGCAGG CCTGCA^GG
SfaNI 255 GCATC GCATC(5/9)
SmlI 1678 CTCAAG C^T[CT][GA]AG
Sse8387I 612 CCTGCAGG CCTGCA^GG
StuI 680 AGGCCT AGG^CCT
TfiI 1759 GATTC G^A[AT]TC
Van91I 1355 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 33 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AocI 676 CCTCAGG CC^T[ACGT]AGG
1613 CCTCAGG
ApaI 156 GGGCCC GGGCC^C
300 GGGCCC
BbvCI 187 CCTCAGC CCTCAGC(-5/-2)
1266 CCTCAGC
BlpI 405 GCTGAGC GC^T[ACGT]AGC
758 GCTCAGC
Bpu1102I 405 GCTGAGC GC^T[ACGT]AGC
758 GCTCAGC
BsaAI 562 TACGTG [CT]AC^GT[GA]
1018 TACGTG
BseRI 129 CTCCTC GAGGAG(10/8)
433 GAGGAG
BsgI 1075 CTGCAC GTGCAG(16/14)
1130 GTGCAG
BspMI 611 ACCTGC ACCTGC(4/8)
1136 ACCTGC
BstAPI 1077 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1232 GCAACGCCTGC
Bsu36I 676 CCTCAGG CC^T[ACGT]AGG
1613 CCTCAGG
CelII 405 GCTGAGC GC^T[ACGT]AGC
758 GCTCAGC
EarI 117 CTCTTC CTCTTC(1/4)
1557 CTCTTC
Eco81I 676 CCTCAGG CC^T[ACGT]AGG
1613 CCTCAGG
HphI 1381 GGTGA GGTGA(8/7)
1632 GGTGA
Ksp632I 117 CTCTTC CTCTTC(1/4)
1557 CTCTTC
MaeIII 1340 GTCAC ^GT[ACGT]AC
1738 GTCAC
MboII 118 TCTTC GAAGA(8/7)
1558 TCTTC
MslI 573 CACCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1533 CATTCCCATG
NaeI 1271 GCCGGC GCC^GGC
1728 GCCGGC
NgoMIV 1271 GCCGGC G^CCGGC
1728 GCCGGC
PpuMI 1122 GGGTCCC [GA]G^G[AT]CC[CT]
1145 AGGACCT
PstI 613 CTGCAG CTGCA^G
1084 CTGCAG
Tsp45I 1340 GTCAC ^GT[GC]AC
1738 GTCAC
Tsp509I 1461 AATT ^AATT
1528 AATT
XcmI 569 CCACCACCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
800 CCAGCCCGCCCATGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI KasI KspI MaeII MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NlaIII NlaIV
NotI PleI RsaI SacII Sau96I
ScrFI SfcI SfoI SmaI StyI
TaqI TseI TspRI TthHB8I XmaCI
XmaI