Restriction map for
pFN21AB0454
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII AccI AclI AflIII AgeI
AocI AscI AseI AsnI Asp700I
AspI AviII AvrII BbeI BciVI
BlnI BsaI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eco81I
EcoRI EcoRV EcoT22I FspI HincII
HindII HpaI KasI KspI MfeI
MluI MunI NarI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfoI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 878 GGTACC G^GTACC
AccIII 166 TCCGGA T^CCGGA
AflII 1402 CTTAAG C^TTAAG
AhdI 2633 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 809 AGCGCT AGC^GCT
Asp718I 878 GGTACC G^GTACC
AspEI 2633 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2165 TGGCCA TGG^CCA
BbrPI 844 CACGTG CAC^GTG
BbvCI 2008 CCTCAGC CCTCAGC(-5/-2)
BcgI 310 GCAGGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 905 TGATCA T^GATCA
BfrI 1402 CTTAAG C^TTAAG
BglII 1370 AGATCT A^GATCT
BsaAI 844 CACGTG [CT]AC^GT[GA]
BsaBI 336 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 166 TCCGGA [AT]^CCGG[AT]
BseAI 166 TCCGGA T^CCGGA
BsgI 1240 GTGCAG GTGCAG(16/14)
BspEI 166 TCCGGA T^CCGGA
BspMI 2664 ACCTGC ACCTGC(4/8)
BstEII 2673 GGTGACC G^GT[ACGT]ACC
BstPI 2673 GGTGACC G^GT[ACGT]ACC
DraIII 1318 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2633 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 809 AGCGCT AGC^GCT
EcoNI 675 CCTCGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2673 GGTGACC G^GT[ACGT]ACC
FbaI 905 TGATCA T^GATCA
FseI 1014 GGCCGGCC GGCCGG^CC
HaeII 809 AGCGCT [GA]GCGC^[CT]
HindIII 1558 AAGCTT A^AGCTT
KpnI 878 GGTACC GGTAC^C
MamI 336 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2165 TGGCCA TGG^CCA
MroI 166 TCCGGA T^CCGGA
MscI 2165 TGGCCA TGG^CCA
NheI 508 GCTAGC G^CTAGC
NspI 1907 ACATGC [GA]CATG^[CT]
PmaCI 844 CACGTG CAC^GTG
PmlI 844 CACGTG CAC^GTG
PpuMI 2751 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 598 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2379 GAGCTC GAGCT^C
SapI 130 GAAGAGC GCTCTTC(1/4)
SexAI 1421 ACCAGGT A^CC[AT]GGT
SgrAI 989 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2568 CCCGGG CCC^GGG
XmaCI 2568 CCCGGG C^CCGGG
XmaI 2568 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 1859 AAATTT [GA]^AATT[CT]
2505 GAATTT
AcyI 40 GACGCC G[GA]^CG[CT]C
2215 GACGCC
Alw44I 1426 GTGCAC G^TGCAC
2356 GTGCAC
ApaLI 1426 GTGCAC G^TGCAC
2356 GTGCAC
ApoI 1859 AAATTT [GA]^AATT[CT]
2505 GAATTT
BamHI 950 GGATCC G^GATCC
1397 GGATCC
BbsI 969 GAAGAC GAAGAC(2/6)
1839 GAAGAC
BfaI 227 CTAG C^TAG
509 CTAG
BlpI 2381 GCTCAGC GC^T[ACGT]AGC
2562 GCTGAGC
Bpu1102I 2381 GCTCAGC GC^T[ACGT]AGC
2562 GCTGAGC
BpuAI 969 GAAGAC GAAGAC(2/6)
1839 GAAGAC
BsaHI 40 GACGCC G[GA]^CG[CT]C
2215 GACGCC
CelII 2381 GCTCAGC GC^T[ACGT]AGC
2562 GCTGAGC
Hin1I 40 GACGCC G[GA]^CG[CT]C
2215 GACGCC
MaeI 227 CTAG C^TAG
509 CTAG
MaeII 845 ACGT A^CGT
1976 ACGT
PstI 1155 CTGCAG CTGCA^G
2082 CTGCAG
SfcI 1155 CTGCAG C^T[GA][CT]AG
2082 CTGCAG
SfiI 426 GGCCGGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2304 GGCCCTCATGGCC
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII