Restriction map for
pFN21AE2183
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflIII AgeI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI Asp700I AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseAI BseRI BsgI
BsiEI BsiWI BsiYI BslI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1260 GGTACC G^GTACC
AflII 928 CTTAAG C^TTAAG
AhdI 175 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1048 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1260 GGTACC G^GTACC
AspEI 175 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1088 GAGCTC G[AT]GC[AT]^C
AvaII 1633 GGACC G^G[AT]CC
BanI 1260 GGTACC G^G[CT][GA]CC
BbsI 1639 GAAGAC GAAGAC(2/6)
BbvCI 973 GCTGAGG CCTCAGC(-5/-2)
BbvI 313 GCAGC GCAGC(8/12)
BfrI 928 CTTAAG C^TTAAG
BpuAI 1639 GAAGAC GAAGAC(2/6)
BsaWI 6 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1088 GAGCTC G[AT]GC[AT]^C
BspHI 53 TCATGA T^CATGA
BssSI 1675 CACGAG CACGAG(-5/-1)
BstAPI 1614 GCAGGTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 1443 TTTAAA TTT^AAA
Eam1105I 175 GACAAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1264 CCTTGG C^C[AT][AT]GG
EcoT22I 112 ATGCAT ATGCA^T
Fnu4HI 313 GCAGC GC^[ACGT]GC
HapII 7 CCGG C^CGG
HpaII 7 CCGG C^CGG
ItaI 313 GCAGC GC^[ACGT]GC
KpnI 1260 GGTACC GGTAC^C
MaeII 938 ACGT A^CGT
MaeIII 571 GTCAC ^GT[ACGT]AC
MslI 93 CATAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 7 CCGG C^CGG
NlaIV 1260 GGTACC GG[ACGT]^[ACGT]CC
NsiI 112 ATGCAT ATGCA^T
PpuMI 1632 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 53 TCATGA T^CATGA
SacI 1088 GAGCTC GAGCT^C
ScaI 1779 AGTACT AGT^ACT
StyI 1264 CCTTGG C^C[AT][AT]GG
TseI 313 GCAGC G^C[AT]GC
Tsp45I 571 GTCAC ^GT[GC]AC
TspRI 1569 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AseI 958 ATTAAT AT^TAAT
1594 ATTAAT
AsnI 958 ATTAAT AT^TAAT
1594 ATTAAT
BanII 997 GAGCCC G[GA]GC[CT]^C
1088 GAGCTC
BmyI 997 GAGCCC G[AGT]GC[ACT]^C
1088 GAGCTC
BpmI 1091 CTCCAG CTGGAG(16/14)
1645 CTCCAG
BsaJI 23 CCCAGG C^C[ACGT][ACGT]GG
1264 CCTTGG
BsmAI 198 GAGAC GTCTC(1/5)
132 GTCTC
Bsp1286I 997 GAGCCC G[AGT]GC[ACT]^C
1088 GAGCTC
BssKI 24 CCAGG ^CC[ACGT]GG
866 CCAGG
BstNI 24 CCAGG CC^[AT]GG
866 CCAGG
Cfr13I 1023 GGCCC G^G[ACGT]CC
1633 GGACC
DraII 1022 AGGCCCT [GA]G^G[ACGT]CC[CT]
1632 AGGACCT
EcoO109I 1022 AGGCCCT [GA]G^G[ACGT]CC[CT]
1632 AGGACCT
EcoRII 24 CCAGG ^CC[AT]GG
866 CCAGG
HincII 35 GTCAAC GT[CT]^[GA]AC
236 GTCAAC
HindII 35 GTCAAC GT[CT]^[GA]AC
236 GTCAAC
MvaI 24 CCAGG CC^[AT]GG
866 CCAGG
PshBI 958 ATTAAT AT^TAAT
1594 ATTAAT
PstI 1250 CTGCAG CTGCA^G
1760 CTGCAG
Sau96I 1023 GGCCC G^G[ACGT]CC
1633 GGACC
ScrFI 24 CCAGG CC^[ACGT]GG
866 CCAGG
SmlI 796 CTCAAG C^T[CT][GA]AG
928 CTTAAG
StuI 768 AGGCCT AGG^CCT
868 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AluI ApoI BfaI
BglII BspMI BsrI BstF5I BstYI
Cac8I DdeI DpnII FokI HaeIII
HinfI HphI MaeI MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIII PleI RsaI Sau3AI SfaNI
SfcI TfiI Tru9I Tsp509I XhoII