Restriction map for
pFN21AE1156
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DrdI DsaI Eam1105I EarI Eco47III
EcoNI EcoO65I EcoRV EcoT14I EcoT22I
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MboII MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 61 GTCTAC GT^[AC][GT]AC
AlwNI 981 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 15 CCTGAGG CC^T[ACGT]AGG
AspHI 426 GAGCTC G[AT]GC[AT]^C
BanII 426 GAGCTC G[GA]GC[CT]^C
BbvCI 181 GCTGAGG CCTCAGC(-5/-2)
BclI 368 TGATCA T^GATCA
BfaI 1102 CTAG C^TAG
BmyI 426 GAGCTC G[AGT]GC[ACT]^C
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsiHKAI 426 GAGCTC G[AT]GC[AT]^C
BsmAI 528 GAGAC GTCTC(1/5)
BsmI 556 GAATGC GAATGC(1/-1)
Bsp1286I 426 GAGCTC G[AGT]GC[ACT]^C
BspHI 851 TCATGA T^CATGA
BsrBI 26 CCGCTC CCGCTC(-3/-3)
BstF5I 51 GGATG GGATG(2/0)
Bsu36I 15 CCTGAGG CC^T[ACGT]AGG
DpnII 369 GATC ^GATC
DraI 1357 TTTAAA TTT^AAA
DraIII 391 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 8 CGGCCG [CT]^GGCC[GA]
EagI 8 CGGCCG C^GGCCG
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
Eco81I 15 CCTGAGG CC^T[ACGT]AGG
FauI 25 CCCGC CCCGC(4/6)
FbaI 368 TGATCA T^GATCA
FokI 51 GGATG GGATG(9/13)
HgaI 97 GACGC GACGC(5/10)
HindIII 743 AAGCTT A^AGCTT
MaeI 1102 CTAG C^TAG
MaeII 550 ACGT A^CGT
MboI 369 GATC ^GATC
MslI 943 CATTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 369 GATC ^GATC
NotI 7 GCGGCCGC GC^GGCCGC
PleI 399 GACTC GAGTC(4/5)
RcaI 851 TCATGA T^CATGA
SacI 426 GAGCTC GAGCT^C
Sau3AI 369 GATC ^GATC
SexAI 392 ACCAGGT A^CC[AT]GGT
SspI 506 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaII 21 GGACC G^G[AT]CC
291 GGTCC
BbvI 249 GCAGC GCAGC(8/12)
980 GCAGC
BsaJI 71 CCCAGG C^C[ACGT][ACGT]GG
176 CCGAGG
BsmFI 20 GGGAC GGGAC(10/14)
292 GTCCC
BssKI 72 CCAGG ^CC[ACGT]GG
393 CCAGG
BstNI 72 CCAGG CC^[AT]GG
393 CCAGG
EcoRI 1092 GAATTC G^AATTC
1260 GAATTC
EcoRII 72 CCAGG ^CC[AT]GG
393 CCAGG
MvaI 72 CCAGG CC^[AT]GG
393 CCAGG
NspI 534 ACATGC [GA]CATG^[CT]
724 ACATGC
PpuMI 20 GGGACCC [GA]G^G[AT]CC[CT]
290 GGGTCCC
ScrFI 72 CCAGG CC^[ACGT]GG
393 CCAGG
SfaNI 126 GATGC GCATC(5/9)
774 GCATC
SfcI 209 CTGTAG C^T[GA][CT]AG
915 CTATAG
SmlI 385 CTCAAG C^T[CT][GA]AG
1011 CTCAAG
TaqI 1197 TCGA T^CGA
1365 TCGA
TfiI 1079 GATTC G^A[AT]TC
1371 GATTC
TseI 249 GCAGC G^C[AT]GC
980 GCAGC
TthHB8I 1197 TCGA T^CGA
1365 TCGA
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AcsI AluI ApoI BpmI
BseRI BsgI BsiYI BslI BsrDI
BsrI Cfr13I DdeI DraII EcoO109I
Fnu4HI HaeIII HinfI HphI ItaI
MaeIII MnlI MseI MwoI NlaIII
NlaIV Sau96I StuI Tru9I Tsp45I
Tsp509I TspRI