Restriction map for
pFN21AB7604
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BalI
BamHI BbrPI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MaeI MaeII MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuII RcaI RsaI SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Aor51HI 74 AGCGCT AGC^GCT
AspHI 952 GAGCTC G[AT]GC[AT]^C
AviII 878 TGCGCA TGC^GCA
BcgI 294 CGAGCTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 525 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 525 GCTCAGC GC^T[ACGT]AGC
BseRI 724 CTCCTC GAGGAG(10/8)
BsiHKAI 952 GAGCTC G[AT]GC[AT]^C
BspMI 817 ACCTGC ACCTGC(4/8)
BsrFI 986 ACCGGC [GA]^CCGG[CT]
BstXI 149 CCATGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 525 GCTCAGC GC^T[ACGT]AGC
Cfr10I 986 ACCGGC [GA]^CCGG[CT]
CpoI 561 CGGTCCG CG^G[AT]CCG
EarI 661 CTCTTC CTCTTC(1/4)
Eco47III 74 AGCGCT AGC^GCT
EcoNI 357 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 149 CCATGG C^C[AT][AT]GG
FspI 878 TGCGCA TGC^GCA
HinfI 97 GAGTC G^A[ACGT]TC
Ksp632I 661 CTCTTC CTCTTC(1/4)
MboII 662 TCTTC GAAGA(8/7)
NcoI 149 CCATGG C^CATGG
NspI 476 GCATGC [GA]CATG^[CT]
PleI 97 GAGTC GAGTC(4/5)
PpuMI 483 AGGTCCC [GA]G^G[AT]CC[CT]
PvuI 1011 CGATCG CGAT^CG
RsrII 561 CGGTCCG CG^G[AT]CCG
SacI 952 GAGCTC GAGCT^C
SbfI 66 CCTGCAGG CCTGCA^GG
SmlI 439 CTCAAG C^T[CT][GA]AG
SphI 476 GCATGC GCATG^C
Sse8387I 66 CCTGCAGG CCTGCA^GG
StyI 149 CCATGG C^C[AT][AT]GG
TaqI 665 TCGA T^CGA
TthHB8I 665 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AscI 235 GGCGCGCC GG^CGCGCC
607 GGCGCGCC
BpmI 748 CTGGAG CTGGAG(16/14)
757 CTGGAG
BsaI 658 GGTCTC GGTCTC(1/5)
721 GGTCTC
BsgI 322 CTGCAC GTGCAG(16/14)
1045 CTGCAC
BsmAI 659 GTCTC GTCTC(1/5)
722 GTCTC
BsrBI 523 CCGCTC CCGCTC(-3/-3)
783 CCGCTC
BsrI 914 ACTGG ACTGG(1/-1)
996 CCAGT
DpnII 1012 GATC ^GATC
1033 GATC
DraII 312 GGGGCCC [GA]G^G[ACGT]CC[CT]
483 AGGTCCC
EcoO109I 312 GGGGCCC [GA]G^G[ACGT]CC[CT]
483 AGGTCCC
HgaI 670 GACGC GACGC(5/10)
739 GCGTC
HphI 892 GGTGA GGTGA(8/7)
1035 TCACC
MboI 1012 GATC ^GATC
1033 GATC
MslI 886 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
992 CATTCCAGTG
NdeII 1012 GATC ^GATC
1033 GATC
NotI 673 GCGGCCGC GC^GGCCGC
776 GCGGCCGC
PstI 67 CTGCAG CTGCA^G
819 CTGCAG
Sau3AI 1012 GATC ^GATC
1033 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BsaHI
BsaJI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EaeI EagI EclXI
Eco52I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI KspI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
SacII Sau96I ScrFI SfaNI SfcI
SfoI SmaI TseI TspRI XmaCI
XmaI