Restriction map for
pFN21AE1614
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# NO CUT ENZYMES ( 200 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 69 CCGC CCGC(-3/-1)
AgeI 107 ACCGGT A^CCGGT
AhdI 314 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 211 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 314 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 110 GGTGCC G^G[CT][GA]CC
BbsI 215 GTCTTC GAAGAC(2/6)
BbvI 253 GCTGC GCAGC(8/12)
BpuAI 215 GTCTTC GAAGAC(2/6)
BsaJI 17 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 107 ACCGGT [AT]^CCGG[AT]
BsiYI 267 CCTGAATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 267 CCTGAATAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrFI 107 ACCGGT [GA]^CCGG[CT]
BstXI 339 CCATGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 107 ACCGGT [GA]^CCGG[CT]
Cfr13I 264 GGGCC G^G[ACGT]CC
DdeI 23 CTCAG C^T[ACGT]AG
Eam1105I 314 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 267 CCTGAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 17 CCAAGG C^C[AT][AT]GG
EcoT22I 361 ATGCAT ATGCA^T
Fnu4HI 253 GCTGC GC^[ACGT]GC
HapII 108 CCGG C^CGG
HpaII 108 CCGG C^CGG
ItaI 253 GCTGC GC^[ACGT]GC
MaeII 329 ACGT A^CGT
MseI 229 TTAA T^TAA
MslI 334 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 108 CCGG C^CGG
MwoI 22 GCTCAGGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 110 GGTGCC GG[ACGT]^[ACGT]CC
NsiI 361 ATGCAT ATGCA^T
PinAI 107 ACCGGT A^CCGGT
Sau96I 264 GGGCC G^G[ACGT]CC
SmlI 137 CTCAAG C^T[CT][GA]AG
StyI 17 CCAAGG C^C[AT][AT]GG
TaqI 282 TCGA T^CGA
Tru9I 229 TTAA T^TAA
TseI 253 GCTGC G^C[AT]GC
TthHB8I 282 TCGA T^CGA
XmnI 211 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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BssKI 37 CCTGG ^CC[ACGT]GG
373 CCTGG
BstF5I 66 CATCC GGATG(2/0)
102 GGATG
BstNI 37 CCTGG CC^[AT]GG
373 CCTGG
EcoRII 37 CCTGG ^CC[AT]GG
373 CCTGG
FokI 66 CATCC GGATG(9/13)
102 GGATG
HphI 159 GGTGA GGTGA(8/7)
291 GGTGA
MvaI 37 CCTGG CC^[AT]GG
373 CCTGG
NlaIII 124 CATG CATG^
340 CATG
ScrFI 37 CCTGG CC^[ACGT]GG
373 CCTGG
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
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DpnII HaeIII MboI MboII MnlI
NdeII Sau3AI