##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tsp45I      
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AflII          94  CTTAAG               C^TTAAG
AlwI           79  GATCC                GGATC(4/5)
BanI          151  GGCACC               G^G[CT][GA]CC
BbvI          179  GCTGC                GCAGC(8/12)
BfaI          131  CTAG                 C^TAG
BfrI           94  CTTAAG               C^TTAAG
BsaI          160  GGTCTC               GGTCTC(1/5)
BsaJI         165  CCCTGG               C^C[ACGT][ACGT]GG
BseRI          16  CTCCTC               GAGGAG(10/8)
BsiYI          49  CCTCTTCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           49  CCTCTTCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         161  GTCTC                GTCTC(1/5)
BspMI          88  ACCTGC               ACCTGC(4/8)
BsrI          118  CCAGT                ACTGG(1/-1)
BstF5I        115  CATCC                GGATG(2/0)
DpnII          79  GATC                 ^GATC
EcoNI          49  CCTCTTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI        179  GCTGC                GC^[ACGT]GC
FokI          115  CATCC                GGATG(9/13)
ItaI          179  GCTGC                GC^[ACGT]GC
MaeI          131  CTAG                 C^TAG
MaeIII         85  GTTAC                ^GT[ACGT]AC
MboI           79  GATC                 ^GATC
MseI           95  TTAA                 T^TAA
NdeI          108  CATATG               CA^TATG
NdeII          79  GATC                 ^GATC
Sau3AI         79  GATC                 ^GATC
SfaNI          45  GATGC                GCATC(5/9)
SmlI           94  CTTAAG               C^T[CT][GA]AG
Tru9I          95  TTAA                 T^TAA
TseI          179  GCTGC                G^C[AT]GC
Tsp509I       147  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BssKI          55  CCAGG                ^CC[ACGT]GG
              166  CCTGG               
BstNI          55  CCAGG                CC^[AT]GG
              166  CCTGG               
EcoRII         55  CCAGG                ^CC[AT]GG
              166  CCTGG               
MvaI           55  CCAGG                CC^[AT]GG
              166  CCTGG               
NlaIV         103  GGAGCC               GG[ACGT]^[ACGT]CC
              151  GGCACC              
ScrFI          55  CCAGG                CC^[ACGT]GG
              166  CCTGG               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

EarI        Ksp632I     MboII       MnlI