##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AcsI          309  GAATTT               [GA]^AATT[CT]
AfaI          101  GTAC                 GT^AC
AlwNI         222  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI            7  GGGCCC               GGGCC^C
ApoI          309  GAATTT               [GA]^AATT[CT]
AspHI         268  GAGCAC               G[AT]GC[AT]^C
BpmI          190  CTCCAG               CTGGAG(16/14)
BsaJI         288  CCATGG               C^C[ACGT][ACGT]GG
BsiHKAI       268  GAGCAC               G[AT]GC[AT]^C
BsmI           70  GCATTC               GAATGC(1/-1)
BsrDI          41  CATTGC               GCAATG(2/0)
Cac8I          94  GCCTGC               GC[ACGT]^[ACGT]GC
DsaI          288  CCATGG               C^C[GA][CT]GG
EcoT14I       288  CCATGG               C^C[AT][AT]GG
HaeIII          8  GGCC                 GG^CC
HinfI         294  GACTC                G^A[ACGT]TC
MslI          150  CATCCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          288  CCATGG               C^CATGG
NlaIII        289  CATG                 CATG^
PleI          294  GACTC                GAGTC(4/5)
RsaI          101  GTAC                 GT^AC
StyI          288  CCATGG               C^C[AT][AT]GG
Tsp509I       310  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BanII           7  GGGCCC               G[GA]GC[CT]^C
               51  GAGCCC              
BsmFI         179  GGGAC                GGGAC(10/14)
              292  GGGAC               
BstAPI         89  GCACTGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              221  GCAGCATCTGC         
Cfr13I          7  GGGCC                G^G[ACGT]CC
                8  GGCCC               
DraII           6  GGGGCCC              [GA]G^G[ACGT]CC[CT]
                7  GGGCCCC             
EcoO109I        6  GGGGCCC              [GA]G^G[ACGT]CC[CT]
                7  GGGCCCC             
HphI          186  TCACC                GGTGA(8/7)
              207  TCACC               
MaeIII        185  GTCAC                ^GT[ACGT]AC
              255  GTCAC               
PstI           21  CTGCAG               CTGCA^G
              163  CTGCAG              
Sau96I          7  GGGCC                G^G[ACGT]CC
                8  GGCCC               
Tsp45I        185  GTCAC                ^GT[GC]AC
              255  GTCAC               
TspRI          90  CACTG                CA[GC]TG[ACGT][ACGT]^
              259  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AluI        BbvI        BmyI        Bsp1286I    BssKI       
BstF5I      BstNI       DdeI        EcoRII      Fnu4HI      
FokI        ItaI        MnlI        MvaI        MwoI        
NlaIV       ScrFI       SfaNI       SfcI        TseI