Restriction map for
pFN21AE1583
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfaI BfrI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI Hin1I HindIII HinfI
HpaI HphI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MspA1I MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AfaI 93 GTAC GT^AC
AflIII 35 ACATGT A^C[GA][CT]GT
AluI 190 AGCT AG^CT
BclI 20 TGATCA T^GATCA
BglI 73 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaJI 83 CCATGG C^C[ACGT][ACGT]GG
BsaWI 8 ACCGGA [AT]^CCGG[AT]
BseRI 250 CTCCTC GAGGAG(10/8)
BsmAI 205 GAGAC GTCTC(1/5)
BspLU11I 35 ACATGT A^CATGT
BsrDI 111 CATTGC GCAATG(2/0)
BssKI 158 CCTGG ^CC[ACGT]GG
BssSI 202 CACGAG CACGAG(-5/-1)
BstF5I 57 GGATG GGATG(2/0)
BstNI 158 CCTGG CC^[AT]GG
Cac8I 78 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 71 GCGC GCG^C
Cfr13I 174 GGGCC G^G[ACGT]CC
DdeI 41 CTGAG C^T[ACGT]AG
DpnII 21 GATC ^GATC
DsaI 83 CCATGG C^C[GA][CT]GG
EarI 253 CTCTTC CTCTTC(1/4)
EcoRII 158 CCTGG ^CC[AT]GG
EcoT14I 83 CCATGG C^C[AT][AT]GG
FbaI 20 TGATCA T^GATCA
FokI 57 GGATG GGATG(9/13)
HapII 9 CCGG C^CGG
HhaI 71 GCGC GCG^C
HinP1I 71 GCGC G^CGC
HincII 64 GTTGAC GT[CT]^[GA]AC
HindII 64 GTTGAC GT[CT]^[GA]AC
HpaII 9 CCGG C^CGG
Ksp632I 253 CTCTTC CTCTTC(1/4)
MaeII 194 ACGT A^CGT
MaeIII 196 GTCAC ^GT[ACGT]AC
MboI 21 GATC ^GATC
MboII 254 TCTTC GAAGA(8/7)
MspI 9 CCGG C^CGG
MvaI 158 CCTGG CC^[AT]GG
MwoI 73 GCCACGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 83 CCATGG C^CATGG
NdeII 21 GATC ^GATC
RsaI 93 GTAC GT^AC
Sau3AI 21 GATC ^GATC
Sau96I 174 GGGCC G^G[ACGT]CC
ScrFI 158 CCTGG CC^[ACGT]GG
SfaNI 58 GATGC GCATC(5/9)
StyI 83 CCATGG C^C[AT][AT]GG
Tsp45I 196 GTCAC ^GT[GC]AC
TspRI 17 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 74 CCACGCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 9 enzymes) #
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AcsI 133 GAATTT [GA]^AATT[CT]
180 AAATTT
ApoI 133 GAATTT [GA]^AATT[CT]
180 AAATTT
BbvI 188 GCAGC GCAGC(8/12)
115 GCTGC
Fnu4HI 115 GCTGC GC^[ACGT]GC
188 GCAGC
HaeIII 81 GGCC GG^CC
175 GGCC
ItaI 115 GCTGC GC^[ACGT]GC
188 GCAGC
NspI 35 ACATGT [GA]CATG^[CT]
47 ACATGC
TseI 115 GCTGC G^C[AT]GC
188 GCAGC
Tsp509I 134 AATT ^AATT
181 AATT
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
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MnlI NlaIII