Restriction map for
pFN21AE1926
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FseI
FspI HaeII HgaI Hin1I HindIII
KasI KpnI MaeI MaeII MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AspHI 721 GAGCTC G[AT]GC[AT]^C
BalI 971 TGGCCA TGG^CCA
BclI 444 TGATCA T^GATCA
BsaI 562 GAGACC GGTCTC(1/5)
BseRI 1030 GAGGAG GAGGAG(10/8)
BsgI 117 CTGCAC GTGCAG(16/14)
BsiHKAI 721 GAGCTC G[AT]GC[AT]^C
BsmI 1195 GCATTC GAATGC(1/-1)
BsrFI 230 ACCGGC [GA]^CCGG[CT]
Cac8I 1267 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 879 GCGC GCG^C
Cfr10I 230 ACCGGC [GA]^CCGG[CT]
DraII 761 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 1177 GACCTCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 963 GAAGAG CTCTTC(1/4)
EcoNI 969 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 761 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1193 ATGCAT ATGCA^T
FbaI 444 TGATCA T^GATCA
HhaI 879 GCGC GCG^C
HinP1I 879 GCGC G^CGC
HincII 742 GTTAAC GT[CT]^[GA]AC
HindII 742 GTTAAC GT[CT]^[GA]AC
HpaI 742 GTTAAC GTT^AAC
Ksp632I 963 GAAGAG CTCTTC(1/4)
KspI 130 CCGCGG CCGC^GG
MluNI 971 TGGCCA TGG^CCA
MscI 971 TGGCCA TGG^CCA
NcoI 935 CCATGG C^CATGG
NsiI 1193 ATGCAT ATGCA^T
NspI 1052 ACATGC [GA]CATG^[CT]
PflMI 606 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 721 GAGCTC GAGCT^C
SacII 130 CCGCGG CCGC^GG
SapI 963 GAAGAGC GCTCTTC(1/4)
StuI 1312 AGGCCT AGG^CCT
Van91I 606 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 131 CGCG CG^CG
880 CGCG
AfaI 852 GTAC GT^AC
1250 GTAC
AlwI 213 GATCC GGATC(4/5)
450 GATCC
AvaII 939 GGTCC G^G[AT]CC
1047 GGTCC
BanI 373 GGTGCC G^G[CT][GA]CC
892 GGCACC
BcnI 171 CCCGG CC^[GC]GG
216 CCCGG
BpmI 391 CTCCAG CTGGAG(16/14)
604 CTCCAG
BsmFI 80 GGGAC GGGAC(10/14)
134 GGGAC
BsrBI 165 CCGCTC CCGCTC(-3/-3)
644 GAGCGG
BstF5I 192 GGATG GGATG(2/0)
1020 GGATG
BstUI 131 CGCG CG^CG
880 CGCG
BstYI 212 AGATCC [GA]^GATC[CT]
449 AGATCC
FokI 192 GGATG GGATG(9/13)
1020 GGATG
MflI 212 AGATCC [GA]^GATC[CT]
449 AGATCC
MvnI 131 CGCG CG^CG
880 CGCG
NciI 171 CCCGG CC^[GC]GG
216 CCCGG
PleI 482 GACTC GAGTC(4/5)
829 GAGTC
PvuII 1096 CAGCTG CAG^CTG
1241 CAGCTG
RsaI 852 GTAC GT^AC
1250 GTAC
SmlI 904 CTTGAG C^T[CT][GA]AG
1213 CTTGAG
TaqI 489 TCGA T^CGA
1118 TCGA
Tsp509I 259 AATT ^AATT
1025 AATT
TthHB8I 489 TCGA T^CGA
1118 TCGA
XhoII 212 AGATCC [GA]^GATC[CT]
449 AGATCC
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwNI BanII BbvI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BssKI BstNI Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
TspRI