Restriction map for
pFN21AE2091
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI AocI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbrPI BbsI BclI BfaI BfrI
BglII BlnI BpuAI BsaBI BsiWI
BsmBI BspDI BspHI BspLU11I BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 1021 GTCTAC GT^[AC][GT]AC
AcsI 1000 GAATTC [GA]^AATT[CT]
AflIII 1412 ACACGT A^C[GA][CT]GT
Alw44I 1558 GTGCAC G^TGCAC
ApaLI 1558 GTGCAC G^TGCAC
ApoI 1000 GAATTC [GA]^AATT[CT]
BbeI 282 GGCGCC GGCGC^C
BglI 1271 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 543 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 543 GCTGAGC GC^T[ACGT]AGC
BsaAI 1413 CACGTA [CT]AC^GT[GA]
Bsp1407I 1775 TGTACA T^GTACA
BsrFI 1157 ACCGGC [GA]^CCGG[CT]
BsrGI 1775 TGTACA T^GTACA
CelII 543 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1157 ACCGGC [GA]^CCGG[CT]
DraIII 1387 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 558 CCTGACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1000 GAATTC G^AATTC
EcoT14I 1919 CCAAGG C^C[AT][AT]GG
KasI 282 GGCGCC G^GCGCC
NarI 282 GGCGCC GG^CGCC
NotI 1650 GCGGCCGC GC^GGCCGC
PvuII 1405 CAGCTG CAG^CTG
ScaI 392 AGTACT AGT^ACT
SexAI 1418 ACCTGGT A^CC[AT]GGT
SfoI 282 GGCGCC GGC^GCC
SspBI 1775 TGTACA T^GTACA
StuI 1892 AGGCCT AGG^CCT
StyI 1919 CCAAGG C^C[AT][AT]GG
Tsp509I 1001 AATT ^AATT
TspRI 2069 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccIII 884 TCCGGA T^CCGGA
2033 TCCGGA
Aor51HI 254 AGCGCT AGC^GCT
1186 AGCGCT
AviII 200 TGCGCA TGC^GCA
1508 TGCGCA
BamHI 758 GGATCC G^GATCC
837 GGATCC
BbvCI 406 GCTGAGG CCTCAGC(-5/-2)
742 GCTGAGG
BcgI 1335 CGAAGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2068 GCAGTGCGCTCG
BciVI 1072 GGATAC GTATCC(6/5)
1906 GTATCC
BsaWI 884 TCCGGA [AT]^CCGG[AT]
2033 TCCGGA
BseAI 884 TCCGGA T^CCGGA
2033 TCCGGA
BsmI 2148 GAATGC GAATGC(1/-1)
2101 GCATTC
BspEI 884 TCCGGA T^CCGGA
2033 TCCGGA
BstXI 1919 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2090 CCACCTTCATGG
EarI 682 CTCTTC CTCTTC(1/4)
2136 GAAGAG
Eco47III 254 AGCGCT AGC^GCT
1186 AGCGCT
FspI 200 TGCGCA TGC^GCA
1508 TGCGCA
Ksp632I 682 CTCTTC CTCTTC(1/4)
2136 GAAGAG
MaeIII 295 GTCAC ^GT[ACGT]AC
1362 GTCAC
MroI 884 TCCGGA T^CCGGA
2033 TCCGGA
MslI 189 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2091 CACCTTCATG
PaeR7I 1972 CTCGAG C^TCGAG
2050 CTCGAG
SapI 2136 GAAGAGC GCTCTTC(1/4)
681 GCTCTTC
Tsp45I 295 GTCAC ^GT[GC]AC
1362 GTCAC
XcmI 599 CCATCGAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1806 CCAGGGGGCCTCTGG
XhoI 1972 CTCGAG C^TCGAG
2050 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI TaqI TfiI TseI TthHB8I
XhoII XmaCI XmaI