Restriction map for
pFN21AE3713
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII Acc65I AccIII AclI Alw44I
ApaLI AscI Asp718I AviII BbeI
BcgI BclI BglI BlpI Bpu1102I
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrGI BssHII CelII ClaI CpoI
EagI EclXI Eco52I EcoT22I FauI
FbaI FseI FspI KasI KpnI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SpeI SphI Sse8387I SspBI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccII 5501 CGCG CG^CG
AcyI 331 GACGCC G[GA]^CG[CT]C
AgeI 3891 ACCGGT A^CCGGT
AhdI 5044 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 950 GGGCCC GGGCC^C
AseI 1192 ATTAAT AT^TAAT
AsnI 1192 ATTAAT AT^TAAT
AspEI 5044 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5760 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5224 CCCGAG C^[CT]CG[GA]G
AvrII 1120 CCTAGG C^CTAGG
BanI 4101 GGTGCC G^G[CT][GA]CC
BbvCI 13 GCTGAGG CCTCAGC(-5/-2)
BcnI 5979 CCCGG CC^[GC]GG
BglII 4616 AGATCT A^GATCT
BlnI 1120 CCTAGG C^CTAGG
BsaBI 2172 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 331 GACGCC G[GA]^CG[CT]C
BsaWI 3891 ACCGGT [AT]^CCGG[AT]
BseRI 2365 GAGGAG GAGGAG(10/8)
BsoBI 5224 CCCGAG C^[CT]CG[GA]G
BspHI 2807 TCATGA T^CATGA
BsrFI 3891 ACCGGT [GA]^CCGG[CT]
Bst1107I 4758 GTATAC GTA^TAC
BstAPI 5851 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3238 TTCGAA TT^CGAA
BstUI 5501 CGCG CG^CG
BstZ17I 4758 GTATAC GTA^TAC
Cfr10I 3891 ACCGGT [GA]^CCGG[CT]
DrdI 6006 GACAGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1968 CCATGG C^C[GA][CT]GG
Eam1105I 5044 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3835 CCTAAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3979 GATATC GAT^ATC
HgaI 331 GACGC GACGC(5/10)
Hin1I 331 GACGCC G[GA]^CG[CT]C
HincII 1836 GTTAAC GT[CT]^[GA]AC
HindII 1836 GTTAAC GT[CT]^[GA]AC
HindIII 1473 AAGCTT A^AGCTT
HpaI 1836 GTTAAC GTT^AAC
MamI 2172 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 5501 CGCG CG^CG
NciI 5979 CCCGG CC^[GC]GG
NcoI 1968 CCATGG C^CATGG
NspV 3238 TTCGAA TT^CGAA
PflFI 5760 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5380 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3891 ACCGGT A^CCGGT
PshBI 1192 ATTAAT AT^TAAT
RcaI 2807 TCATGA T^CATGA
SacI 812 GAGCTC GAGCT^C
ScaI 2042 AGTACT AGT^ACT
SfuI 3238 TTCGAA TT^CGAA
SnaBI 3954 TACGTA TAC^GTA
SspI 2661 AATATT AAT^ATT
StuI 1532 AGGCCT AGG^CCT
Tth111I 5760 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 5380 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 4764 CCAGAAAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 2419 GTAGAC GT^[AC][GT]AC
4758 GTATAC
AflII 2373 CTTAAG C^TTAAG
4552 CTTAAG
AflIII 4010 ACGTGT A^C[GA][CT]GT
4377 ACACGT
AocI 997 CCTGAGG CC^T[ACGT]AGG
1654 CCTGAGG
Aor51HI 1522 AGCGCT AGC^GCT
5971 AGCGCT
BfrI 2373 CTTAAG C^TTAAG
4552 CTTAAG
BsaI 4567 GAGACC GGTCTC(1/5)
3819 GGTCTC
BsmI 1918 GAATGC GAATGC(1/-1)
4170 GAATGC
BssSI 1848 CACGAG CACGAG(-5/-1)
5881 CACGAG
BstEII 805 GGTAACC G^GT[ACGT]ACC
4189 GGTCACC
BstPI 805 GGTAACC G^GT[ACGT]ACC
4189 GGTCACC
BstXI 3913 CCAACTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4571 CCAGGGACCTGG
Bsu36I 997 CCTGAGG CC^T[ACGT]AGG
1654 CCTGAGG
DraI 3453 TTTAAA TTT^AAA
3640 TTTAAA
Eco47III 1522 AGCGCT AGC^GCT
5971 AGCGCT
Eco81I 997 CCTGAGG CC^T[ACGT]AGG
1654 CCTGAGG
EcoO65I 805 GGTAACC G^GT[ACGT]ACC
4189 GGTCACC
HaeII 1522 AGCGCT [GA]GCGC^[CT]
5971 AGCGCT
NspI 1026 GCATGT [GA]CATG^[CT]
2670 GCATGT
PpuMI 4574 GGGACCT [GA]G^G[AT]CC[CT]
5764 AGGTCCT
SapI 2401 GAAGAGC GCTCTTC(1/4)
6065 GCTCTTC
SexAI 4017 ACCAGGT A^CC[AT]GGT
5761 ACCAGGT
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BamHI BanII BbrPI BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NdeII NlaIII NlaIV
PleI PmaCI PmlI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI