Restriction map for
pFN21AE0966
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaI BsaWI
BseAI BsiEI BsmI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI DraIII
DrdI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
HindIII HpaI KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 70 enzymes) #
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AccII 1410 CGCG CG^CG
AcyI 6 GGCGCC G[GA]^CG[CT]C
AflIII 1154 ACATGT A^C[GA][CT]GT
AhdI 667 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1294 GTGCAC G^TGCAC
AlwI 37 GGATC GGATC(4/5)
AocI 1099 CCTGAGG CC^T[ACGT]AGG
Aor51HI 140 AGCGCT AGC^GCT
ApaI 360 GGGCCC GGGCC^C
ApaLI 1294 GTGCAC G^TGCAC
AspEI 667 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 876 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1145 TGCGCA TGC^GCA
BbeI 6 GGCGCC GGCGC^C
BfaI 235 CTAG C^TAG
BglI 1003 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 372 CACGTA [CT]AC^GT[GA]
BsaHI 6 GGCGCC G[GA]^CG[CT]C
BsgI 169 CTGCAC GTGCAG(16/14)
BsiWI 374 CGTACG C^GTACG
BspHI 626 TCATGA T^CATGA
BspLU11I 1154 ACATGT A^CATGT
BsrDI 741 CATTGC GCAATG(2/0)
BstAPI 317 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1488 GGTGACC G^GT[ACGT]ACC
BstPI 1488 GGTGACC G^GT[ACGT]ACC
BstUI 1410 CGCG CG^CG
BstXI 1163 CCATGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1099 CCTGAGG CC^T[ACGT]AGG
DraI 964 TTTAAA TTT^AAA
Eam1105I 667 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 140 AGCGCT AGC^GCT
Eco81I 1099 CCTGAGG CC^T[ACGT]AGG
EcoNI 900 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1488 GGTGACC G^GT[ACGT]ACC
EcoT14I 1163 CCATGG C^C[AT][AT]GG
FspI 1145 TGCGCA TGC^GCA
Hin1I 6 GGCGCC G[GA]^CG[CT]C
HincII 1453 GTCAAC GT[CT]^[GA]AC
HindII 1453 GTCAAC GT[CT]^[GA]AC
KasI 6 GGCGCC G^GCGCC
KspI 1409 CCGCGG CCGC^GG
MaeI 235 CTAG C^TAG
MaeII 373 ACGT A^CGT
MvnI 1410 CGCG CG^CG
NaeI 49 GCCGGC GCC^GGC
NarI 6 GGCGCC GG^CGCC
NcoI 1163 CCATGG C^CATGG
NgoMIV 49 GCCGGC G^CCGGC
PaeR7I 1108 CTCGAG C^TCGAG
PflFI 876 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 349 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1525 GAGTC GAGTC(4/5)
PpuMI 521 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1491 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 571 CAGCTG CAG^CTG
RcaI 626 TCATGA T^CATGA
SacI 211 GAGCTC GAGCT^C
SacII 1409 CCGCGG CCGC^GG
SapI 987 GAAGAGC GCTCTTC(1/4)
SfoI 6 GGCGCC GGC^GCC
SmaI 1341 CCCGGG CCC^GGG
StuI 888 AGGCCT AGG^CCT
StyI 1163 CCATGG C^C[AT][AT]GG
TfiI 1440 GATTC G^A[AT]TC
Tth111I 876 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 349 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1108 CTCGAG C^TCGAG
XmaCI 1341 CCCGGG C^CCGGG
XmaI 1341 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 350 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
571 CAGCTGCTG
BalI 272 TGGCCA TGG^CCA
1254 TGGCCA
BglII 650 AGATCT A^GATCT
695 AGATCT
BsmBI 874 GAGACG CGTCTC(1/5)
1083 GAGACG
BsrBI 129 GAGCGG CCGCTC(-3/-3)
1426 GAGCGG
BsrFI 49 GCCGGC [GA]^CCGG[CT]
479 ACCGGC
BsrI 104 ACTGG ACTGG(1/-1)
1328 ACTGG
BstYI 650 AGATCT [GA]^GATC[CT]
695 AGATCT
Cfr10I 49 GCCGGC [GA]^CCGG[CT]
479 ACCGGC
EaeI 272 TGGCCA [CT]^GGCC[GA]
1254 TGGCCA
EarI 987 GAAGAG CTCTTC(1/4)
1246 GAAGAG
FauI 1411 GCGGG CCCGC(4/6)
1428 GCGGG
HinfI 1440 GATTC G^A[ACGT]TC
1525 GAGTC
HphI 1488 GGTGA GGTGA(8/7)
1499 TCACC
Ksp632I 987 GAAGAG CTCTTC(1/4)
1246 GAAGAG
MflI 650 AGATCT [GA]^GATC[CT]
695 AGATCT
MluNI 272 TGGCCA TGG^CCA
1254 TGGCCA
MscI 272 TGGCCA TGG^CCA
1254 TGGCCA
MseI 965 TTAA T^TAA
1319 TTAA
MspA1I 571 CAGCTG C[AC]G^C[GT]G
1409 CCGCGG
NspI 1154 ACATGT [GA]CATG^[CT]
1229 ACATGC
SmlI 829 CTCAAG C^T[CT][GA]AG
1108 CTCGAG
Tru9I 965 TTAA T^TAA
1319 TTAA
XcmI 101 CCAACTGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
516 CCATGAGGACCCTGG
XhoII 650 AGATCT [GA]^GATC[CT]
695 AGATCT
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BssKI BssSI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I