Restriction map for
pFN21AE4120
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI Asp700I Asp718I AspEI AspHI
AspI AvrII BalI BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiEI BsiHKAI BsiWI BsmFI BspDI
BspEI BspHI BssHII BssSI BstBI
BstEII BstPI BstUI BstXI CelII
ClaI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FseI
HaeII HgaI Hin1I HpaI KasI
KpnI KspI MaeI MaeII MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflIII 179 ACATGT A^C[GA][CT]GT
AlwNI 358 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 568 CCTGAGG CC^T[ACGT]AGG
AseI 385 ATTAAT AT^TAAT
AsnI 385 ATTAAT AT^TAAT
AvaI 1415 CTCGAG C^[CT]CG[GA]G
AviII 74 TGCGCA TGC^GCA
BbsI 876 GTCTTC GAAGAC(2/6)
BpuAI 876 GTCTTC GAAGAC(2/6)
BsaI 441 GAGACC GGTCTC(1/5)
BsaWI 1100 TCCGGT [AT]^CCGG[AT]
BsgI 800 GTGCAG GTGCAG(16/14)
BsmI 371 GCATTC GAATGC(1/-1)
BsoBI 1415 CTCGAG C^[CT]CG[GA]G
Bsp1407I 352 TGTACA T^GTACA
BspLU11I 179 ACATGT A^CATGT
BspMI 1120 ACCTGC ACCTGC(4/8)
BsrDI 1036 GCAATG GCAATG(2/0)
BsrFI 518 ACCGGC [GA]^CCGG[CT]
BsrGI 352 TGTACA T^GTACA
BstAPI 1124 GCATCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 996 GGATG GGATG(2/0)
BstYI 359 AGATCC [GA]^GATC[CT]
Bsu36I 568 CCTGAGG CC^T[ACGT]AGG
Cfr10I 518 ACCGGC [GA]^CCGG[CT]
CpoI 680 CGGACCG CG^G[AT]CCG
DraII 1377 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 136 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 898 CGGCCA [CT]^GGCC[GA]
Eco81I 568 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1377 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 996 GGATG GGATG(9/13)
FspI 74 TGCGCA TGC^GCA
HincII 916 GTTGAC GT[CT]^[GA]AC
HindII 916 GTTGAC GT[CT]^[GA]AC
HindIII 122 AAGCTT A^AGCTT
MfeI 22 CAATTG C^AATTG
MflI 359 AGATCC [GA]^GATC[CT]
MunI 22 CAATTG C^AATTG
PaeR7I 1415 CTCGAG C^TCGAG
PshBI 385 ATTAAT AT^TAAT
RsrII 680 CGGACCG CG^G[AT]CCG
SfcI 848 CTGTAG C^T[GA][CT]AG
SmlI 1415 CTCGAG C^T[CT][GA]AG
SphI 77 GCATGC GCATG^C
SspBI 352 TGTACA T^GTACA
TaqI 1416 TCGA T^CGA
TthHB8I 1416 TCGA T^CGA
XhoI 1415 CTCGAG C^TCGAG
XhoII 359 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1227 GTATAC GT^[AC][GT]AC
1364 GTATAC
AvaII 681 GGACC G^G[AT]CC
1164 GGACC
BbvCI 394 GCTGAGG CCTCAGC(-5/-2)
1426 GCTGAGG
BglI 886 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
929 GCCCCTCCGGC
BseRI 571 GAGGAG GAGGAG(10/8)
751 GAGGAG
BsmBI 88 CGTCTC CGTCTC(1/5)
1114 CGTCTC
BsrBI 480 CCGCTC CCGCTC(-3/-3)
1090 GAGCGG
Bst1107I 1227 GTATAC GTA^TAC
1364 GTATAC
BstZ17I 1227 GTATAC GTA^TAC
1364 GTATAC
EcoRV 292 GATATC GAT^ATC
547 GATATC
MseI 386 TTAA T^TAA
412 TTAA
MslI 1288 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1294 CATGTCTGTG
MspA1I 311 CAGCTG C[AC]G^C[GT]G
1243 CAGCTG
NcoI 818 CCATGG C^CATGG
840 CCATGG
PvuII 311 CAGCTG CAG^CTG
1243 CAGCTG
Tru9I 386 TTAA T^TAA
412 TTAA
Tsp45I 1408 GTCAC ^GT[GC]AC
1437 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT14I Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI TseI Tsp509I TspRI