Restriction map for
pFN21AE4089
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbsI BciVI BclI
BfrI BglII BlnI BpuAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 684 GTAC GT^AC
AflIII 672 ACATGT A^C[GA][CT]GT
AlwNI 1234 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1317 CCTCAGG CC^T[ACGT]AGG
AscI 798 GGCGCGCC GG^CGCGCC
AviII 713 TGCGCA TGC^GCA
BbeI 697 GGCGCC GGCGC^C
BbrPI 643 CACGTG CAC^GTG
BbvCI 928 GCTGAGG CCTCAGC(-5/-2)
BcgI 530 CGACTCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 403 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 403 GCTGAGC GC^T[ACGT]AGC
BsaAI 643 CACGTG [CT]AC^GT[GA]
BsiEI 836 CGGCCG CG[GA][CT]^CG
BsmBI 631 GAGACG CGTCTC(1/5)
BspLU11I 672 ACATGT A^CATGT
BsrBI 1312 CCGCTC CCGCTC(-3/-3)
BstAPI 1044 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1162 CCAGAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1317 CCTCAGG CC^T[ACGT]AGG
CelII 403 GCTGAGC GC^T[ACGT]AGC
DraIII 744 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 172 GACCCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 959 CCATGG C^C[GA][CT]GG
EagI 836 CGGCCG C^GGCCG
EclXI 836 CGGCCG C^GGCCG
Eco52I 836 CGGCCG C^GGCCG
Eco81I 1317 CCTCAGG CC^T[ACGT]AGG
FspI 713 TGCGCA TGC^GCA
HgaI 633 GACGC GACGC(5/10)
KasI 697 GGCGCC G^GCGCC
MaeIII 914 GTTAC ^GT[ACGT]AC
MseI 1109 TTAA T^TAA
NarI 697 GGCGCC GG^CGCC
NcoI 959 CCATGG C^CATGG
NspI 672 ACATGT [GA]CATG^[CT]
PmaCI 643 CACGTG CAC^GTG
PmlI 643 CACGTG CAC^GTG
PpuMI 932 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 684 GTAC GT^AC
SfoI 697 GGCGCC GGC^GCC
TaqI 381 TCGA T^CGA
Tru9I 1109 TTAA T^TAA
TthHB8I 381 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 633 GACGCC G[GA]^CG[CT]C
697 GGCGCC
AlwI 390 GATCC GGATC(4/5)
552 GATCC
BfaI 149 CTAG C^TAG
1249 CTAG
BglI 283 GCCTGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
829 GCCCGGGCGGC
BsaHI 633 GACGCC G[GA]^CG[CT]C
697 GGCGCC
DpnII 390 GATC ^GATC
552 GATC
EaeI 836 CGGCCG [CT]^GGCC[GA]
896 TGGCCG
EarI 193 CTCTTC CTCTTC(1/4)
238 GAAGAG
EcoNI 51 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
207 CCTGCAGCAGG
HaeII 697 GGCGCC [GA]GCGC^[CT]
848 AGCGCC
Hin1I 633 GACGCC G[GA]^CG[CT]C
697 GGCGCC
Ksp632I 193 CTCTTC CTCTTC(1/4)
238 GAAGAG
MaeI 149 CTAG C^TAG
1249 CTAG
MaeII 644 ACGT A^CGT
704 ACGT
MboI 390 GATC ^GATC
552 GATC
MboII 238 GAAGA GAAGA(8/7)
194 TCTTC
MslI 252 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
954 CACGCCCATG
NaeI 803 GCCGGC GCC^GGC
1280 GCCGGC
NdeII 390 GATC ^GATC
552 GATC
NgoMIV 803 GCCGGC G^CCGGC
1280 GCCGGC
Sau3AI 390 GATC ^GATC
552 GATC
SmaI 134 CCCGGG CCC^GGG
830 CCCGGG
StuI 151 AGGCCT AGG^CCT
446 AGGCCT
XcmI 248 CCAACATGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
575 CCAAGTTGCTGCTGG
XmaCI 134 CCCGGG C^CCGGG
830 CCCGGG
XmaI 134 CCCGGG C^CCGGG
830 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI PstI PvuII Sau96I
ScrFI SfaNI SfcI StyI TseI
TspRI