Restriction map for
pFN21AE4034
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII CpoI
DpnII DraI DraIII Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI HphI KpnI MaeIII
MamI MboI MfeI MflI MluI
MroI MseI MunI NcoI NdeI
NdeII NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SalI Sau3AI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tru9I
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 608 GTCTAC GT^[AC][GT]AC
Alw44I 697 GTGCAC G^TGCAC
ApaLI 697 GTGCAC G^TGCAC
BalI 548 TGGCCA TGG^CCA
BbsI 84 GAAGAC GAAGAC(2/6)
BcnI 123 CCGGG CC^[GC]GG
BfaI 379 CTAG C^TAG
BpmI 214 CTGGAG CTGGAG(16/14)
BpuAI 84 GAAGAC GAAGAC(2/6)
BsgI 502 CTGCAC GTGCAG(16/14)
BspDI 619 ATCGAT AT^CGAT
BsrBI 109 GAGCGG CCGCTC(-3/-3)
BsrDI 657 CATTGC GCAATG(2/0)
BsrFI 207 GCCGGC [GA]^CCGG[CT]
BsrI 413 ACTGG ACTGG(1/-1)
BstAPI 504 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 575 CCATGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 207 GCCGGC [GA]^CCGG[CT]
ClaI 619 ATCGAT AT^CGAT
DdeI 565 CTGAG C^T[ACGT]AG
DrdI 529 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 564 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 345 GCGTC GACGC(5/10)
HinfI 460 GACTC G^A[ACGT]TC
MaeI 379 CTAG C^TAG
MaeII 274 ACGT A^CGT
MluNI 548 TGGCCA TGG^CCA
MscI 548 TGGCCA TGG^CCA
MslI 576 CATGAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 207 GCCGGC GCC^GGC
NciI 123 CCGGG CC^[GC]GG
NgoMIV 207 GCCGGC G^CCGGC
NlaIII 576 CATG CATG^
NotI 9 GCGGCCGC GC^GGCCGC
PleI 460 GACTC GAGTC(4/5)
SacI 721 GAGCTC GAGCT^C
SbfI 664 CCTGCAGG CCTGCA^GG
Sse8387I 664 CCTGCAGG CCTGCA^GG
StuI 131 AGGCCT AGG^CCT
TaqI 620 TCGA T^CGA
Tsp509I 384 AATT ^AATT
TthHB8I 620 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 3 GGCGCC G[GA]^CG[CT]C
43 GGCGCC
ApaI 18 GGGCCC GGGCC^C
488 GGGCCC
AvaI 163 CCCGAG C^[CT]CG[GA]G
184 CCCGAG
BbeI 3 GGCGCC GGCGC^C
43 GGCGCC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
43 GGCGCC
BsoBI 163 CCCGAG C^[CT]CG[GA]G
184 CCCGAG
EarI 70 CTCTTC CTCTTC(1/4)
682 GAAGAG
EcoT14I 23 CCTTGG C^C[AT][AT]GG
356 CCAAGG
HapII 123 CCGG C^CGG
208 CCGG
Hin1I 3 GGCGCC G[GA]^CG[CT]C
43 GGCGCC
HpaII 123 CCGG C^CGG
208 CCGG
KasI 3 GGCGCC G^GCGCC
43 GGCGCC
Ksp632I 70 CTCTTC CTCTTC(1/4)
682 GAAGAG
MspI 123 CCGG C^CGG
208 CCGG
NarI 3 GGCGCC GG^CGCC
43 GGCGCC
PstI 592 CTGCAG CTGCA^G
665 CTGCAG
SapI 682 GAAGAGC GCTCTTC(1/4)
69 GCTCTTC
SfoI 3 GGCGCC GGC^GCC
43 GGCGCC
StyI 23 CCTTGG C^C[AT][AT]GG
356 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII BanI BanII BbvI BmyI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I ItaI KspI MboII MnlI
MspA1I MvaI MvnI MwoI NlaIV
PpuMI SacII Sau96I ScrFI SfcI
TseI