Restriction map for
pFN21AB6257
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaII
AvrII BamHI BanI BbeI BbrPI
BbvCI BciVI BcnI BfrI BglI
BglII BlnI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI HaeII Hin1I
HpaI KasI KpnI KspI MaeII
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AocI 22 CCTCAGG CC^T[ACGT]AGG
AseI 831 ATTAAT AT^TAAT
AsnI 831 ATTAAT AT^TAAT
Asp700I 1680 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 535 GCAATGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 581 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 581 GCTTAGC GC^T[ACGT]AGC
BsaBI 1570 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1462 GGTCTC GGTCTC(1/5)
BsmBI 1831 CGTCTC CGTCTC(1/5)
BstXI 2039 CCATCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 22 CCTCAGG CC^T[ACGT]AGG
CelII 581 GCTTAGC GC^T[ACGT]AGC
DraII 1278 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eco81I 22 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1278 AGGCCCC [GA]G^G[ACGT]CC[CT]
HapII 475 CCGG C^CGG
HincII 427 GTTGAC GT[CT]^[GA]AC
HindII 427 GTTGAC GT[CT]^[GA]AC
HindIII 526 AAGCTT A^AGCTT
HpaII 475 CCGG C^CGG
MamI 1570 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 475 CCGG C^CGG
NdeI 1246 CATATG CA^TATG
NheI 1035 GCTAGC G^CTAGC
PflMI 2104 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 831 ATTAAT AT^TAAT
SacI 457 GAGCTC GAGCT^C
SapI 454 GAAGAGC GCTCTTC(1/4)
ScaI 1973 AGTACT AGT^ACT
SmlI 2085 CTCAAG C^T[CT][GA]AG
Van91I 2104 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1083 CCAAGGCAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1680 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 574 CCCGAG C^[CT]CG[GA]G
1637 CCCGAG
AviII 1919 TGCGCA TGC^GCA
1946 TGCGCA
BalI 234 TGGCCA TGG^CCA
719 TGGCCA
BanII 136 GGGCTC G[GA]GC[CT]^C
457 GAGCTC
BbsI 42 GTCTTC GAAGAC(2/6)
294 GTCTTC
BclI 614 TGATCA T^GATCA
855 TGATCA
BpuAI 42 GTCTTC GAAGAC(2/6)
294 GTCTTC
BsmFI 568 GGGAC GGGAC(10/14)
1297 GTCCC
BsmI 1680 GAATGC GAATGC(1/-1)
1118 GCATTC
BsoBI 574 CCCGAG C^[CT]CG[GA]G
1637 CCCGAG
BstAPI 970 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1959 GCAGGAAATGC
Cfr13I 1000 GGCCC G^G[ACGT]CC
1279 GGCCC
EaeI 234 TGGCCA [CT]^GGCC[GA]
719 TGGCCA
FbaI 614 TGATCA T^GATCA
855 TGATCA
FspI 1919 TGCGCA TGC^GCA
1946 TGCGCA
MluNI 234 TGGCCA TGG^CCA
719 TGGCCA
MscI 234 TGGCCA TGG^CCA
719 TGGCCA
MslI 606 CATGGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2040 CATCGCCATG
NspI 162 GCATGT [GA]CATG^[CT]
1739 ACATGC
PstI 872 CTGCAG CTGCA^G
1012 CTGCAG
Sau96I 1000 GGCCC G^G[ACGT]CC
1279 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BbvI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI DdeI DpnII
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NcoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII