Restriction map for
pFN21AE4085
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BbsI BbvCI
BciVI BclI BfaI BfrI BlnI
BpuAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KpnI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 616 GTCTAC GT^[AC][GT]AC
BbeI 1156 GGCGCC GGCGC^C
BbrPI 706 CACGTG CAC^GTG
BcgI 904 CGAGGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 605 AGATCT A^GATCT
BsaAI 706 CACGTG [CT]AC^GT[GA]
BsaWI 513 ACCGGA [AT]^CCGG[AT]
BssHII 291 GCGCGC G^CGCGC
BssSI 436 CACGAG CACGAG(-5/-1)
BstXI 56 CCAGCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 605 AGATCT [GA]^GATC[CT]
DraIII 997 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1087 CGGCCA [CT]^GGCC[GA]
EarI 775 CTCTTC CTCTTC(1/4)
EcoT14I 1248 CCATGG C^C[AT][AT]GG
KasI 1156 GGCGCC G^GCGCC
Ksp632I 775 CTCTTC CTCTTC(1/4)
MaeII 707 ACGT A^CGT
MaeIII 987 GTCAC ^GT[ACGT]AC
MflI 605 AGATCT [GA]^GATC[CT]
NarI 1156 GGCGCC GG^CGCC
NcoI 1248 CCATGG C^CATGG
PmaCI 706 CACGTG CAC^GTG
PmlI 706 CACGTG CAC^GTG
SacI 79 GAGCTC GAGCT^C
SbfI 480 CCTGCAGG CCTGCA^GG
ScaI 846 AGTACT AGT^ACT
SexAI 23 ACCTGGT A^CC[AT]GGT
SfoI 1156 GGCGCC GGC^GCC
SmaI 1268 CCCGGG CCC^GGG
SmlI 490 CTCAAG C^T[CT][GA]AG
Sse8387I 480 CCTGCAGG CCTGCA^GG
StyI 1248 CCATGG C^C[AT][AT]GG
Tsp45I 987 GTCAC ^GT[GC]AC
Tsp509I 425 AATT ^AATT
XcmI 1313 CCACCTCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 605 AGATCT [GA]^GATC[CT]
XmaCI 1268 CCCGGG C^CCGGG
XmaI 1268 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 709 GTGCAC G^TGCAC
994 GTGCAC
AocI 454 CCTCAGG CC^T[ACGT]AGG
1165 CCTGAGG
ApaLI 709 GTGCAC G^TGCAC
994 GTGCAC
BlpI 81 GCTCAGC GC^T[ACGT]AGC
137 GCTCAGC
Bpu1102I 81 GCTCAGC GC^T[ACGT]AGC
137 GCTCAGC
BsaI 380 GAGACC GGTCTC(1/5)
397 GAGACC
BsiEI 111 CGACCG CG[GA][CT]^CG
511 CGACCG
BsrBI 783 CCGCTC CCGCTC(-3/-3)
1015 GAGCGG
Bsu36I 454 CCTCAGG CC^T[ACGT]AGG
1165 CCTGAGG
CelII 81 GCTCAGC GC^T[ACGT]AGC
137 GCTCAGC
DpnII 606 GATC ^GATC
1035 GATC
Eco81I 454 CCTCAGG CC^T[ACGT]AGG
1165 CCTGAGG
HaeII 882 GGCGCT [GA]GCGC^[CT]
1156 GGCGCC
HgaI 664 GACGC GACGC(5/10)
734 GCGTC
KspI 1213 CCGCGG CCGC^GG
1233 CCGCGG
MboI 606 GATC ^GATC
1035 GATC
MboII 336 GAAGA GAAGA(8/7)
776 TCTTC
NdeII 606 GATC ^GATC
1035 GATC
PflMI 6 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCAGCAGCTGG
SacII 1213 CCGCGG CCGC^GG
1233 CCGCGG
Sau3AI 606 GATC ^GATC
1035 GATC
SfiI 1041 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1289 GGCCCCCCAGGCC
TfiI 122 GATTC G^A[AT]TC
1063 GATTC
Van91I 6 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1131 CCAGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau96I ScrFI
SfaNI SfcI StuI TaqI TseI
TspRI TthHB8I