Restriction map for
pFN21AE4165
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII Eam1105I EarI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MfeI MluI
MroI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SfiI SfuI SnaBI
SpeI SspI SwaI Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 304 GACGTC GACGT^C
AccI 988 GTCTAC GT^[AC][GT]AC
AflIII 1058 ACACGT A^C[GA][CT]GT
Alw44I 111 GTGCAC G^TGCAC
AlwI 1406 GGATC GGATC(4/5)
AocI 1370 CCTCAGG CC^T[ACGT]AGG
ApaLI 111 GTGCAC G^TGCAC
BbeI 1473 GGCGCC GGCGC^C
BbsI 628 GTCTTC GAAGAC(2/6)
BglI 870 GCCCTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1679 AGATCT A^GATCT
BpuAI 628 GTCTTC GAAGAC(2/6)
BsaBI 1680 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 487 GAGACC GGTCTC(1/5)
Bsp1407I 1739 TGTACA T^GTACA
BsrDI 1310 GCAATG GCAATG(2/0)
BsrGI 1739 TGTACA T^GTACA
BssHII 726 GCGCGC G^CGCGC
BssSI 883 CACGAG CACGAG(-5/-1)
BstXI 476 CCATGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1370 CCTCAGG CC^T[ACGT]AGG
DrdI 967 GACCGCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1370 CCTCAGG CC^T[ACGT]AGG
EcoNI 144 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1505 CCATGG C^C[AT][AT]GG
FseI 1638 GGCCGGCC GGCCGG^CC
KasI 1473 GGCGCC G^GCGCC
MamI 1680 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 206 TTAA T^TAA
NarI 1473 GGCGCC GG^CGCC
NcoI 1505 CCATGG C^CATGG
NspI 284 GCATGC [GA]CATG^[CT]
PpuMI 1769 GGGACCT [GA]G^G[AT]CC[CT]
SacI 1027 GAGCTC GAGCT^C
ScaI 1589 AGTACT AGT^ACT
SexAI 860 ACCAGGT A^CC[AT]GGT
SfoI 1473 GGCGCC GGC^GCC
SgrAI 276 CACCGGCG C[GA]^CCGG[CT]G
SmlI 106 CTCAAG C^T[CT][GA]AG
SphI 284 GCATGC GCATG^C
SspBI 1739 TGTACA T^GTACA
StyI 1505 CCATGG C^C[AT][AT]GG
TfiI 1170 GAATC G^A[AT]TC
Tru9I 206 TTAA T^TAA
Tsp509I 1081 AATT ^AATT
XcmI 518 CCACCTTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BalI 158 TGGCCA TGG^CCA
512 TGGCCA
BsmBI 1708 CGTCTC CGTCTC(1/5)
1603 GAGACG
BsmFI 1769 GGGAC GGGAC(10/14)
1834 GGGAC
BstYI 1406 GGATCT [GA]^GATC[CT]
1679 AGATCT
MflI 1406 GGATCT [GA]^GATC[CT]
1679 AGATCT
MluNI 158 TGGCCA TGG^CCA
512 TGGCCA
MscI 158 TGGCCA TGG^CCA
512 TGGCCA
PvuII 994 CAGCTG CAG^CTG
1219 CAGCTG
SbfI 147 CCTGCAGG CCTGCA^GG
1185 CCTGCAGG
SmaI 60 CCCGGG CCC^GGG
1826 CCCGGG
Sse8387I 147 CCTGCAGG CCTGCA^GG
1185 CCTGCAGG
StuI 215 AGGCCT AGG^CCT
848 AGGCCT
XhoII 1406 GGATCT [GA]^GATC[CT]
1679 AGATCT
XmaCI 60 CCCGGG C^CCGGG
1826 CCCGGG
XmaI 60 CCCGGG C^CCGGG
1826 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbrPI BbvI BcnI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NotI PleI
PmaCI PmlI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I TspRI TthHB8I