################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SfiI SfuI SnaBI SpeI SspI SwaI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 304 GACGTC GACGT^C AccI 988 GTCTAC GT^[AC][GT]AC AflIII 1058 ACACGT A^C[GA][CT]GT Alw44I 111 GTGCAC G^TGCAC AlwI 1406 GGATC GGATC(4/5) AocI 1370 CCTCAGG CC^T[ACGT]AGG ApaLI 111 GTGCAC G^TGCAC BbeI 1473 GGCGCC GGCGC^C BbsI 628 GTCTTC GAAGAC(2/6) BglI 870 GCCCTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1679 AGATCT A^GATCT BpuAI 628 GTCTTC GAAGAC(2/6) BsaBI 1680 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 487 GAGACC GGTCTC(1/5) Bsp1407I 1739 TGTACA T^GTACA BsrDI 1310 GCAATG GCAATG(2/0) BsrGI 1739 TGTACA T^GTACA BssHII 726 GCGCGC G^CGCGC BssSI 883 CACGAG CACGAG(-5/-1) BstXI 476 CCATGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1370 CCTCAGG CC^T[ACGT]AGG DrdI 967 GACCGCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1370 CCTCAGG CC^T[ACGT]AGG EcoNI 144 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1505 CCATGG C^C[AT][AT]GG FseI 1638 GGCCGGCC GGCCGG^CC KasI 1473 GGCGCC G^GCGCC MamI 1680 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 206 TTAA T^TAA NarI 1473 GGCGCC GG^CGCC NcoI 1505 CCATGG C^CATGG NspI 284 GCATGC [GA]CATG^[CT] PpuMI 1769 GGGACCT [GA]G^G[AT]CC[CT] SacI 1027 GAGCTC GAGCT^C ScaI 1589 AGTACT AGT^ACT SexAI 860 ACCAGGT A^CC[AT]GGT SfoI 1473 GGCGCC GGC^GCC SgrAI 276 CACCGGCG C[GA]^CCGG[CT]G SmlI 106 CTCAAG C^T[CT][GA]AG SphI 284 GCATGC GCATG^C SspBI 1739 TGTACA T^GTACA StyI 1505 CCATGG C^C[AT][AT]GG TfiI 1170 GAATC G^A[AT]TC Tru9I 206 TTAA T^TAA Tsp509I 1081 AATT ^AATT XcmI 518 CCACCTTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BalI 158 TGGCCA TGG^CCA 512 TGGCCA BsmBI 1708 CGTCTC CGTCTC(1/5) 1603 GAGACG BsmFI 1769 GGGAC GGGAC(10/14) 1834 GGGAC BstYI 1406 GGATCT [GA]^GATC[CT] 1679 AGATCT MflI 1406 GGATCT [GA]^GATC[CT] 1679 AGATCT MluNI 158 TGGCCA TGG^CCA 512 TGGCCA MscI 158 TGGCCA TGG^CCA 512 TGGCCA PvuII 994 CAGCTG CAG^CTG 1219 CAGCTG SbfI 147 CCTGCAGG CCTGCA^GG 1185 CCTGCAGG SmaI 60 CCCGGG CCC^GGG 1826 CCCGGG Sse8387I 147 CCTGCAGG CCTGCA^GG 1185 CCTGCAGG StuI 215 AGGCCT AGG^CCT 848 AGGCCT XhoII 1406 GGATCT [GA]^GATC[CT] 1679 AGATCT XmaCI 60 CCCGGG C^CCGGG 1826 CCCGGG XmaI 60 CCCGGG C^CCGGG 1826 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbrPI BbvI BcnI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NotI PleI PmaCI PmlI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I