################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI AscI Asp718I AspI AviII BamHI BanI BbeI BbrPI BcgI BciVI BclI BglI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI BspEI BspLU11I BspMI BsrBI BsrFI BssHII Bst1107I BstAPI BstXI BstZ17I CelII Cfr10I CpoI DraIII DrdI EagI EclXI Eco52I EcoNI EcoRI EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AflII 1623 CTTAAG C^TTAAG AhdI 363 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1451 GTGCAC G^TGCAC AlwI 2087 GGATC GGATC(4/5) AocI 1769 CCTTAGG CC^T[ACGT]AGG Aor51HI 2426 AGCGCT AGC^GCT ApaLI 1451 GTGCAC G^TGCAC AseI 1458 ATTAAT AT^TAAT AsnI 1458 ATTAAT AT^TAAT Asp700I 1378 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 363 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 14 CCCGAG C^[CT]CG[GA]G AvrII 103 CCTAGG C^CTAGG BbvCI 109 CCTCAGC CCTCAGC(-5/-2) BcnI 554 CCCGG CC^[GC]GG BfrI 1623 CTTAAG C^TTAAG BglII 462 AGATCT A^GATCT BlnI 103 CCTAGG C^CTAGG BsgI 795 CTGCAC GTGCAG(16/14) BsmBI 2114 GAGACG CGTCTC(1/5) BsoBI 14 CCCGAG C^[CT]CG[GA]G Bsp1407I 1726 TGTACA T^GTACA BspDI 1891 ATCGAT AT^CGAT BsrGI 1726 TGTACA T^GTACA BssSI 1873 CTCGTG CACGAG(-5/-1) BstBI 821 TTCGAA TT^CGAA BstEII 2304 GGTTACC G^GT[ACGT]ACC BstPI 2304 GGTTACC G^GT[ACGT]ACC BstYI 462 AGATCT [GA]^GATC[CT] Bsu36I 1769 CCTTAGG CC^T[ACGT]AGG ClaI 1891 ATCGAT AT^CGAT DraI 1546 TTTAAA TTT^AAA Eam1105I 363 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 443 CTCTTC CTCTTC(1/4) Eco47III 2426 AGCGCT AGC^GCT Eco81I 1769 CCTTAGG CC^T[ACGT]AGG EcoO65I 2304 GGTTACC G^GT[ACGT]ACC EcoRV 198 GATATC GAT^ATC HaeII 2426 AGCGCT [GA]GCGC^[CT] Ksp632I 443 CTCTTC CTCTTC(1/4) KspI 39 CCGCGG CCGC^GG MfeI 713 CAATTG C^AATTG MflI 462 AGATCT [GA]^GATC[CT] MunI 713 CAATTG C^AATTG NciI 554 CCCGG CC^[GC]GG NcoI 1562 CCATGG C^CATGG NspV 821 TTCGAA TT^CGAA PshBI 1458 ATTAAT AT^TAAT SacII 39 CCGCGG CCGC^GG SapI 442 GCTCTTC GCTCTTC(1/4) SexAI 87 ACCTGGT A^CC[AT]GGT SfuI 821 TTCGAA TT^CGAA SmlI 1623 CTTAAG C^T[CT][GA]AG SphI 1568 GCATGC GCATG^C SspBI 1726 TGTACA T^GTACA SspI 637 AATATT AAT^ATT StuI 106 AGGCCT AGG^CCT XhoII 462 AGATCT [GA]^GATC[CT] XmnI 1378 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwNI 1130 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 2005 CAGGAGCTG ApaI 35 GGGCCC GGGCC^C 1110 GGGCCC AvaII 972 GGACC G^G[AT]CC 1433 GGTCC BbsI 428 GAAGAC GAAGAC(2/6) 2261 GAAGAC BpuAI 428 GAAGAC GAAGAC(2/6) 2261 GAAGAC BsaBI 576 GATAACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1408 GATTACAATC BseRI 236 GAGGAG GAGGAG(10/8) 1838 GAGGAG BspHI 258 TCATGA T^CATGA 665 TCATGA BsrDI 1797 CATTGC GCAATG(2/0) 2066 GCAATG CfoI 55 GCGC GCG^C 2427 GCGC HhaI 55 GCGC GCG^C 2427 GCGC HinP1I 55 GCGC G^CGC 2427 GCGC MaeII 803 ACGT A^CGT 1202 ACGT MamI 576 GATAACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1408 GATTACAATC NspI 1568 GCATGC [GA]CATG^[CT] 2075 ACATGC PflMI 2275 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2505 CCACAAGCTGG RcaI 258 TCATGA T^CATGA 665 TCATGA SacI 1153 GAGCTC GAGCT^C 1843 GAGCTC Van91I 2275 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2505 CCACAAGCTGG XcmI 195 CCAGATATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1474 CCACTAGATAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI AspHI BalI BanII BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I