Restriction map for
pFN21AE9627
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AscI Asp718I
AspI AviII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BglI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstXI BstZ17I
CelII Cfr10I CpoI DraIII DrdI
EagI EclXI Eco52I EcoNI EcoRI
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AflII 1623 CTTAAG C^TTAAG
AhdI 363 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1451 GTGCAC G^TGCAC
AlwI 2087 GGATC GGATC(4/5)
AocI 1769 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2426 AGCGCT AGC^GCT
ApaLI 1451 GTGCAC G^TGCAC
AseI 1458 ATTAAT AT^TAAT
AsnI 1458 ATTAAT AT^TAAT
Asp700I 1378 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 363 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 14 CCCGAG C^[CT]CG[GA]G
AvrII 103 CCTAGG C^CTAGG
BbvCI 109 CCTCAGC CCTCAGC(-5/-2)
BcnI 554 CCCGG CC^[GC]GG
BfrI 1623 CTTAAG C^TTAAG
BglII 462 AGATCT A^GATCT
BlnI 103 CCTAGG C^CTAGG
BsgI 795 CTGCAC GTGCAG(16/14)
BsmBI 2114 GAGACG CGTCTC(1/5)
BsoBI 14 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1726 TGTACA T^GTACA
BspDI 1891 ATCGAT AT^CGAT
BsrGI 1726 TGTACA T^GTACA
BssSI 1873 CTCGTG CACGAG(-5/-1)
BstBI 821 TTCGAA TT^CGAA
BstEII 2304 GGTTACC G^GT[ACGT]ACC
BstPI 2304 GGTTACC G^GT[ACGT]ACC
BstYI 462 AGATCT [GA]^GATC[CT]
Bsu36I 1769 CCTTAGG CC^T[ACGT]AGG
ClaI 1891 ATCGAT AT^CGAT
DraI 1546 TTTAAA TTT^AAA
Eam1105I 363 GACATCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 443 CTCTTC CTCTTC(1/4)
Eco47III 2426 AGCGCT AGC^GCT
Eco81I 1769 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2304 GGTTACC G^GT[ACGT]ACC
EcoRV 198 GATATC GAT^ATC
HaeII 2426 AGCGCT [GA]GCGC^[CT]
Ksp632I 443 CTCTTC CTCTTC(1/4)
KspI 39 CCGCGG CCGC^GG
MfeI 713 CAATTG C^AATTG
MflI 462 AGATCT [GA]^GATC[CT]
MunI 713 CAATTG C^AATTG
NciI 554 CCCGG CC^[GC]GG
NcoI 1562 CCATGG C^CATGG
NspV 821 TTCGAA TT^CGAA
PshBI 1458 ATTAAT AT^TAAT
SacII 39 CCGCGG CCGC^GG
SapI 442 GCTCTTC GCTCTTC(1/4)
SexAI 87 ACCTGGT A^CC[AT]GGT
SfuI 821 TTCGAA TT^CGAA
SmlI 1623 CTTAAG C^T[CT][GA]AG
SphI 1568 GCATGC GCATG^C
SspBI 1726 TGTACA T^GTACA
SspI 637 AATATT AAT^ATT
StuI 106 AGGCCT AGG^CCT
XhoII 462 AGATCT [GA]^GATC[CT]
XmnI 1378 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 1130 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2005 CAGGAGCTG
ApaI 35 GGGCCC GGGCC^C
1110 GGGCCC
AvaII 972 GGACC G^G[AT]CC
1433 GGTCC
BbsI 428 GAAGAC GAAGAC(2/6)
2261 GAAGAC
BpuAI 428 GAAGAC GAAGAC(2/6)
2261 GAAGAC
BsaBI 576 GATAACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1408 GATTACAATC
BseRI 236 GAGGAG GAGGAG(10/8)
1838 GAGGAG
BspHI 258 TCATGA T^CATGA
665 TCATGA
BsrDI 1797 CATTGC GCAATG(2/0)
2066 GCAATG
CfoI 55 GCGC GCG^C
2427 GCGC
HhaI 55 GCGC GCG^C
2427 GCGC
HinP1I 55 GCGC G^CGC
2427 GCGC
MaeII 803 ACGT A^CGT
1202 ACGT
MamI 576 GATAACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1408 GATTACAATC
NspI 1568 GCATGC [GA]CATG^[CT]
2075 ACATGC
PflMI 2275 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2505 CCACAAGCTGG
RcaI 258 TCATGA T^CATGA
665 TCATGA
SacI 1153 GAGCTC GAGCT^C
1843 GAGCTC
Van91I 2275 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2505 CCACAAGCTGG
XcmI 195 CCAGATATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1474 CCACTAGATAACTGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI BalI BanII BbvI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I