Restriction map for
pFN21AE9626
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AviII BalI BamHI
BbeI BbrPI BbvCI BciVI BfrI
BglI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiHKAI BsmI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
BstBI BstEII BstPI BstXI Bsu36I
CelII Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FseI FspI
HaeII Hin1I HindIII HpaI KasI
KspI MfeI MluNI MroI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 2275 GGTACC G^GTACC
AccII 1234 CGCG CG^CG
AlwNI 1872 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 29 GGGCCC GGGCC^C
Asp718I 2275 GGTACC G^GTACC
AvaI 386 CTCGGG C^[CT]CG[GA]G
AvrII 175 CCTAGG C^CTAGG
BclI 1094 TGATCA T^GATCA
BglII 1111 AGATCT A^GATCT
BlnI 175 CCTAGG C^CTAGG
BsaAI 2371 TACGTA [CT]AC^GT[GA]
BseRI 1670 CTCCTC GAGGAG(10/8)
BsiWI 2373 CGTACG C^GTACG
BsmBI 828 GAGACG CGTCTC(1/5)
BsmFI 1307 GGGAC GGGAC(10/14)
BsoBI 386 CTCGGG C^[CT]CG[GA]G
BspDI 652 ATCGAT AT^CGAT
BsrBI 551 CCGCTC CCGCTC(-3/-3)
BsrDI 2504 GCAATG GCAATG(2/0)
Bst1107I 948 GTATAC GTA^TAC
BstUI 1234 CGCG CG^CG
BstZ17I 948 GTATAC GTA^TAC
CfoI 928 GCGC GCG^C
ClaI 652 ATCGAT AT^CGAT
DraIII 1634 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 903 CGGCCA [CT]^GGCC[GA]
EcoRV 2444 GATATC GAT^ATC
FbaI 1094 TGATCA T^GATCA
HgaI 1232 GACGC GACGC(5/10)
HhaI 928 GCGC GCG^C
HinP1I 928 GCGC G^CGC
KpnI 2275 GGTACC GGTAC^C
MluI 1233 ACGCGT A^CGCGT
MvnI 1234 CGCG CG^CG
NdeI 2231 CATATG CA^TATG
NspI 1310 ACATGC [GA]CATG^[CT]
PpuMI 18 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 166 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2327 CTGCAG CTGCA^G
ScaI 414 AGTACT AGT^ACT
SexAI 2423 ACCTGGT A^CC[AT]GGT
SmlI 1525 CTCAAG C^T[CT][GA]AG
SnaBI 2371 TACGTA TAC^GTA
StuI 862 AGGCCT AGG^CCT
XcmI 86 CCATTAAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 376 AAATTT [GA]^AATT[CT]
772 AAATTC
AflIII 1233 ACGCGT A^C[GA][CT]GT
1602 ACGTGT
ApoI 376 AAATTT [GA]^AATT[CT]
772 AAATTC
Asp700I 1320 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1404 GAAGGGTTTC
BanII 29 GGGCCC G[GA]GC[CT]^C
1507 GAGCCC
BbsI 56 GAAGAC GAAGAC(2/6)
1575 GAAGAC
BcgI 654 CGATGTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2065 CGAGAAGCCTGC
BcnI 1570 CCGGG CC^[GC]GG
1941 CCGGG
BpmI 554 CTCCAG CTGGAG(16/14)
1255 CTCCAG
BpuAI 56 GAAGAC GAAGAC(2/6)
1575 GAAGAC
BsaBI 757 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1449 GATGCCCATC
BsaI 239 GAGACC GGTCTC(1/5)
1899 GAGACC
Bsp1407I 2380 TGTACA T^GTACA
2538 TGTACA
BsrGI 2380 TGTACA T^GTACA
2538 TGTACA
BstAPI 399 GCACCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1108 GCAAGATCTGC
DdeI 437 CTGAG C^T[ACGT]AG
1436 CTAAG
DraI 220 TTTAAA TTT^AAA
1198 TTTAAA
DraII 18 AGGACCC [GA]G^G[ACGT]CC[CT]
28 GGGGCCC
EarI 953 CTCTTC CTCTTC(1/4)
2127 GAAGAG
EcoO109I 18 AGGACCC [GA]G^G[ACGT]CC[CT]
28 GGGGCCC
FauI 22 CCCGC CCCGC(4/6)
969 CCCGC
HincII 199 GTTGAC GT[CT]^[GA]AC
1968 GTCAAC
HindII 199 GTTGAC GT[CT]^[GA]AC
1968 GTCAAC
Ksp632I 953 CTCTTC CTCTTC(1/4)
2127 GAAGAG
MamI 757 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1449 GATGCCCATC
NciI 1570 CCGGG CC^[GC]GG
1941 CCGGG
PleI 202 GACTC GAGTC(4/5)
810 GACTC
SspBI 2380 TGTACA T^GTACA
2538 TGTACA
XmnI 1320 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1404 GAAGGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AvaII BanI BbvI BfaI BmyI
BsaJI BsgI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DpnII
DsaI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV PflMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII