Restriction map for
pFN21AE3540
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
AlwI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AfaI 831 GTAC GT^AC
AflIII 453 ACATGT A^C[GA][CT]GT
ApaI 534 GGGCCC GGGCC^C
AspI 196 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 419 TGGCCA TGG^CCA
BfaI 434 CTAG C^TAG
BsaI 67 GAGACC GGTCTC(1/5)
BseRI 519 CTCCTC GAGGAG(10/8)
BsmBI 221 GAGACG CGTCTC(1/5)
BsmFI 675 GTCCC GGGAC(10/14)
BspLU11I 453 ACATGT A^CATGT
DdeI 522 CTCAG C^T[ACGT]AG
EarI 550 CTCTTC CTCTTC(1/4)
EcoT14I 530 CCATGG C^C[AT][AT]GG
HaeII 131 GGCGCT [GA]GCGC^[CT]
Ksp632I 550 CTCTTC CTCTTC(1/4)
MaeI 434 CTAG C^TAG
MaeIII 461 GTGAC ^GT[ACGT]AC
MluNI 419 TGGCCA TGG^CCA
MscI 419 TGGCCA TGG^CCA
MslI 454 CATGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 530 CCATGG C^CATGG
NspI 453 ACATGT [GA]CATG^[CT]
PflFI 196 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsaI 831 GTAC GT^AC
ScaI 830 AGTACT AGT^ACT
SfiI 10 GGCCCCGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 127 CACCGGCG C[GA]^CCGG[CT]G
SmlI 589 CTCAAG C^T[CT][GA]AG
StyI 530 CCATGG C^C[AT][AT]GG
TfiI 486 GAATC G^A[AT]TC
Tsp45I 461 GTGAC ^GT[GC]AC
Tth111I 196 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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Alw44I 210 GTGCAC G^TGCAC
847 GTGCAC
ApaLI 210 GTGCAC G^TGCAC
847 GTGCAC
AvaI 762 CCCGAG C^[CT]CG[GA]G
871 CCCGAG
AvaII 360 GGTCC G^G[AT]CC
642 GGACC
BanII 4 GAGCCC G[GA]GC[CT]^C
534 GGGCCC
BbsI 579 GAAGAC GAAGAC(2/6)
157 GTCTTC
BclI 293 TGATCA T^GATCA
506 TGATCA
BcnI 7 CCCGG CC^[GC]GG
85 CCCGG
BpuAI 579 GAAGAC GAAGAC(2/6)
157 GTCTTC
BsoBI 762 CCCGAG C^[CT]CG[GA]G
871 CCCGAG
BspHI 476 TCATGA T^CATGA
575 TCATGA
BsrFI 128 ACCGGC [GA]^CCGG[CT]
783 GCCGGT
Cac8I 134 GCTCGC GC[ACGT]^[ACGT]GC
880 GCCCGC
Cfr10I 128 ACCGGC [GA]^CCGG[CT]
783 GCCGGT
DrdI 148 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
376 GACGAGGATGTC
EcoNI 321 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
645 CCTCATCAAGG
FbaI 293 TGATCA T^GATCA
506 TGATCA
HphI 333 GGTGA GGTGA(8/7)
460 GGTGA
NciI 7 CCCGG CC^[GC]GG
85 CCCGG
PvuII 115 CAGCTG CAG^CTG
823 CAGCTG
RcaI 476 TCATGA T^CATGA
575 TCATGA
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
BbvI BglI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI CfoI Cfr13I DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TspRI TthHB8I