Restriction map for
pFN21AE9519
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbrPI BciVI
BclI BfrI BlnI BsaBI BseRI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrGI BsrI BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI DraI DraIII
DrdI Eam1105I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MamI MfeI MluI
MluNI MscI MseI MunI NdeI
NheI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SexAI
SfiI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 472 GTCTAC GT^[AC][GT]AC
AccIII 1059 TCCGGA T^CCGGA
AflIII 296 ACGTGT A^C[GA][CT]GT
ApaI 710 GGGCCC GGGCC^C
AviII 395 TGCGCA TGC^GCA
BbvCI 991 CCTCAGC CCTCAGC(-5/-2)
BcgI 110 GCAACGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 879 AGATCT A^GATCT
BsaAI 295 TACGTG [CT]AC^GT[GA]
BsaI 1028 GAGACC GGTCTC(1/5)
BseAI 1059 TCCGGA T^CCGGA
BspEI 1059 TCCGGA T^CCGGA
BspMI 895 GCAGGT ACCTGC(4/8)
BsrDI 1022 GCAATG GCAATG(2/0)
BssHII 302 GCGCGC G^CGCGC
BstBI 922 TTCGAA TT^CGAA
BstF5I 1043 GGATG GGATG(2/0)
BstYI 879 AGATCT [GA]^GATC[CT]
CpoI 1056 CGGTCCG CG^G[AT]CCG
DpnII 880 GATC ^GATC
EarI 919 CTCTTC CTCTTC(1/4)
EcoNI 732 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 1043 GGATG GGATG(9/13)
FspI 395 TGCGCA TGC^GCA
HinfI 555 GACTC G^A[ACGT]TC
Ksp632I 919 CTCTTC CTCTTC(1/4)
KspI 1017 CCGCGG CCGC^GG
MboI 880 GATC ^GATC
MflI 879 AGATCT [GA]^GATC[CT]
MroI 1059 TCCGGA T^CCGGA
MslI 174 CACGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 971 CCATGG C^CATGG
NdeII 880 GATC ^GATC
NotI 950 GCGGCCGC GC^GGCCGC
NspI 311 GCATGT [GA]CATG^[CT]
NspV 922 TTCGAA TT^CGAA
PleI 555 GACTC GAGTC(4/5)
RsrII 1056 CGGTCCG CG^G[AT]CCG
SacI 976 GAGCTC GAGCT^C
SacII 1017 CCGCGG CCGC^GG
SapI 918 GCTCTTC GCTCTTC(1/4)
Sau3AI 880 GATC ^GATC
SfaNI 588 GATGC GCATC(5/9)
SfuI 922 TTCGAA TT^CGAA
StuI 254 AGGCCT AGG^CCT
TaqI 923 TCGA T^CGA
TspRI 35 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 923 TCGA T^CGA
XhoII 879 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 384 GGCGCC G[GA]^CG[CT]C
628 GGCGCC
AfaI 291 GTAC GT^AC
315 GTAC
Aor51HI 353 AGCGCT AGC^GCT
750 AGCGCT
AspHI 856 GTGCTC G[AT]GC[AT]^C
976 GAGCTC
BanI 384 GGCGCC G^G[CT][GA]CC
628 GGCGCC
BbeI 384 GGCGCC GGCGC^C
628 GGCGCC
BbsI 48 GAAGAC GAAGAC(2/6)
865 GTCTTC
BfaI 842 CTAG C^TAG
959 CTAG
BpmI 550 CTGGAG CTGGAG(16/14)
875 CTGGAG
BpuAI 48 GAAGAC GAAGAC(2/6)
865 GTCTTC
BsaHI 384 GGCGCC G[GA]^CG[CT]C
628 GGCGCC
BsaWI 762 ACCGGA [AT]^CCGG[AT]
1059 TCCGGA
BsgI 149 CTGCAC GTGCAG(16/14)
184 CTGCAC
BsiHKAI 856 GTGCTC G[AT]GC[AT]^C
976 GAGCTC
DsaI 971 CCATGG C^C[GA][CT]GG
1017 CCGCGG
EagI 464 CGGCCG C^GGCCG
951 CGGCCG
EclXI 464 CGGCCG C^GGCCG
951 CGGCCG
Eco47III 353 AGCGCT AGC^GCT
750 AGCGCT
Eco52I 464 CGGCCG C^GGCCG
951 CGGCCG
HgaI 609 GACGC GACGC(5/10)
845 GCGTC
Hin1I 384 GGCGCC G[GA]^CG[CT]C
628 GGCGCC
HincII 727 GTCAAC GT[CT]^[GA]AC
847 GTCAAC
HindII 727 GTCAAC GT[CT]^[GA]AC
847 GTCAAC
KasI 384 GGCGCC G^GCGCC
628 GGCGCC
MaeI 842 CTAG C^TAG
959 CTAG
MaeII 296 ACGT A^CGT
863 ACGT
MaeIII 367 GTCAC ^GT[ACGT]AC
607 GTGAC
NaeI 668 GCCGGC GCC^GGC
793 GCCGGC
NarI 384 GGCGCC GG^CGCC
628 GGCGCC
NgoMIV 668 GCCGGC G^CCGGC
793 GCCGGC
NlaIII 312 CATG CATG^
972 CATG
PpuMI 897 AGGTCCC [GA]G^G[AT]CC[CT]
1067 GGGACCT
PstI 349 CTGCAG CTGCA^G
736 CTGCAG
PvuII 505 CAGCTG CAG^CTG
994 CAGCTG
RsaI 291 GTAC GT^AC
315 GTAC
SfoI 384 GGCGCC GGC^GCC
628 GGCGCC
SmaI 890 CCCGGG CCC^GGG
1032 CCCGGG
Tsp45I 367 GTCAC ^GT[GC]AC
607 GTGAC
XmaCI 890 CCCGGG C^CCGGG
1032 CCCGGG
XmaI 890 CCCGGG C^CCGGG
1032 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanII BbvI BcnI BglI BlpI
BmyI Bpu1102I BsaJI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BssKI BstNI BstUI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
Sau96I ScrFI SfcI StyI TseI