Restriction map for
pFN21AE3032
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BsaBI BsaWI BseAI
BseRI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HapII HincII
HindII HindIII HpaI HpaII KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 253 GGCGCC G[GA]^CG[CT]C
AlwI 359 GGATC GGATC(4/5)
AspHI 211 GTGCTC G[AT]GC[AT]^C
AvaII 290 GGTCC G^G[AT]CC
BanI 253 GGCGCC G^G[CT][GA]CC
BbeI 253 GGCGCC GGCGC^C
BbsI 435 GAAGAC GAAGAC(2/6)
BlpI 213 GCTCAGC GC^T[ACGT]AGC
BmyI 211 GTGCTC G[AGT]GC[ACT]^C
BpmI 226 CTGGAG CTGGAG(16/14)
Bpu1102I 213 GCTCAGC GC^T[ACGT]AGC
BpuAI 435 GAAGAC GAAGAC(2/6)
BsaAI 238 TACGTG [CT]AC^GT[GA]
BsaHI 253 GGCGCC G[GA]^CG[CT]C
BsaI 136 GAGACC GGTCTC(1/5)
BsgI 91 CTGCAC GTGCAG(16/14)
BsiEI 33 CGACCG CG[GA][CT]^CG
BsiHKAI 211 GTGCTC G[AT]GC[AT]^C
BsmAI 136 GAGAC GTCTC(1/5)
BsmFI 354 GGGAC GGGAC(10/14)
Bsp1286I 211 GTGCTC G[AGT]GC[ACT]^C
BssHII 22 GCGCGC G^CGCGC
BstEII 459 GGTCACC G^GT[ACGT]ACC
BstF5I 153 CATCC GGATG(2/0)
BstPI 459 GGTCACC G^GT[ACGT]ACC
BstXI 257 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 359 GGATCT [GA]^GATC[CT]
CelII 213 GCTCAGC GC^T[ACGT]AGC
DpnII 360 GATC ^GATC
DraIII 94 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 339 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 459 GGTCACC G^GT[ACGT]ACC
FokI 153 CATCC GGATG(9/13)
HgaI 438 GACGC GACGC(5/10)
Hin1I 253 GGCGCC G[GA]^CG[CT]C
HinfI 109 GACTC G^A[ACGT]TC
KasI 253 GGCGCC G^GCGCC
MaeIII 460 GTCAC ^GT[ACGT]AC
MboI 360 GATC ^GATC
MboII 435 GAAGA GAAGA(8/7)
MflI 359 GGATCT [GA]^GATC[CT]
MseI 422 TTAA T^TAA
NarI 253 GGCGCC GG^CGCC
NdeII 360 GATC ^GATC
NlaIV 253 GGCGCC GG[ACGT]^[ACGT]CC
PleI 109 GACTC GAGTC(4/5)
PstI 10 CTGCAG CTGCA^G
Sau3AI 360 GATC ^GATC
SexAI 95 ACCAGGT A^CC[AT]GGT
SfoI 253 GGCGCC GGC^GCC
TaqI 74 TCGA T^CGA
Tru9I 422 TTAA T^TAA
Tsp45I 460 GTCAC ^GT[GC]AC
TthHB8I 74 TCGA T^CGA
XhoII 359 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
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BsrI 77 ACTGG ACTGG(1/-1)
324 CCAGT
Cfr13I 290 GGTCC G^G[ACGT]CC
348 GGGCC
DdeI 28 CTTAG C^T[ACGT]AG
214 CTCAG
DsaI 47 CCGTGG C^C[GA][CT]GG
305 CCGTGG
HaeII 253 GGCGCC [GA]GCGC^[CT]
295 AGCGCC
HaeIII 312 GGCC GG^CC
349 GGCC
MaeII 83 ACGT A^CGT
239 ACGT
MnlI 165 CCTC CCTC(7/6)
310 GAGG
Sau96I 290 GGTCC G^G[ACGT]CC
348 GGGCC
SfcI 10 CTGCAG C^T[GA][CT]AG
363 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiYI BslI BssKI BstNI BstUI
Cac8I CfoI EcoRII FauI Fnu4HI
HhaI HinP1I HphI ItaI MslI
MvaI MvnI MwoI NlaIII ScrFI
TseI