Restriction map for
pFN21AE1151
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BbrPI BcgI
BciVI BfaI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MfeI MluI MluNI
MroI MscI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI StuI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 160 GTCTAC GT^[AC][GT]AC
AcsI 1262 AAATTT [GA]^AATT[CT]
AfaI 1173 GTAC GT^AC
AflII 855 CTTAAG C^TTAAG
AocI 108 CCTCAGG CC^T[ACGT]AGG
Aor51HI 539 AGCGCT AGC^GCT
ApoI 1262 AAATTT [GA]^AATT[CT]
AviII 929 TGCGCA TGC^GCA
BamHI 885 GGATCC G^GATCC
BbvCI 600 GCTGAGG CCTCAGC(-5/-2)
BclI 1449 TGATCA T^GATCA
BfrI 855 CTTAAG C^TTAAG
BsaBI 1447 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 937 GTGCAG GTGCAG(16/14)
BsmAI 639 GAGAC GTCTC(1/5)
BsmFI 964 GTCCC GGGAC(10/14)
BspMI 926 ACCTGC ACCTGC(4/8)
BsrBI 22 GAGCGG CCGCTC(-3/-3)
BstAPI 636 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 108 CCTCAGG CC^T[ACGT]AGG
EaeI 93 CGGCCA [CT]^GGCC[GA]
Eco47III 539 AGCGCT AGC^GCT
Eco81I 108 CCTCAGG CC^T[ACGT]AGG
FbaI 1449 TGATCA T^GATCA
FspI 929 TGCGCA TGC^GCA
HphI 1258 GGTGA GGTGA(8/7)
MaeII 488 ACGT A^CGT
MamI 1447 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 856 TTAA T^TAA
MslI 804 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 826 GCATGC [GA]CATG^[CT]
PpuMI 302 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 1173 GTAC GT^AC
SacI 850 GAGCTC GAGCT^C
SbfI 66 CCTGCAGG CCTGCA^GG
SexAI 416 ACCAGGT A^CC[AT]GGT
SmlI 855 CTTAAG C^T[CT][GA]AG
SphI 826 GCATGC GCATG^C
Sse8387I 66 CCTGCAGG CCTGCA^GG
Tru9I 856 TTAA T^TAA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 99 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
637 CAGAGACTG
AspHI 367 GAGCAC G[AT]GC[AT]^C
850 GAGCTC
BbeI 9 GGCGCC GGCGC^C
387 GGCGCC
BbsI 819 GAAGAC GAAGAC(2/6)
1325 GTCTTC
BpuAI 819 GAAGAC GAAGAC(2/6)
1325 GTCTTC
BsiHKAI 367 GAGCAC G[AT]GC[AT]^C
850 GAGCTC
BsrI 1040 ACTGG ACTGG(1/-1)
1212 ACTGG
BssHII 146 GCGCGC G^CGCGC
287 GCGCGC
EarI 1202 CTCTTC CTCTTC(1/4)
1315 CTCTTC
EcoT14I 809 CCATGG C^C[AT][AT]GG
952 CCATGG
KasI 9 GGCGCC G^GCGCC
387 GGCGCC
Ksp632I 1202 CTCTTC CTCTTC(1/4)
1315 CTCTTC
MaeIII 781 GTCAC ^GT[ACGT]AC
1279 GTCAC
NarI 9 GGCGCC GG^CGCC
387 GGCGCC
NcoI 809 CCATGG C^CATGG
952 CCATGG
PvuII 220 CAGCTG CAG^CTG
598 CAGCTG
SfoI 9 GGCGCC GGC^GCC
387 GGCGCC
SmaI 16 CCCGGG CCC^GGG
271 CCCGGG
StyI 809 CCATGG C^C[AT][AT]GG
952 CCATGG
Tsp45I 781 GTCAC ^GT[GC]AC
1279 GTCAC
Tsp509I 1263 AATT ^AATT
1402 AATT
XcmI 212 CCAACCGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1311 CCAACTCTTCTCTGG
XmaCI 16 CCCGGG C^CCGGG
271 CCCGGG
XmaI 16 CCCGGG C^CCGGG
271 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsoBI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI TspRI XhoII