Restriction map for
pFN21AB5805
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# NO CUT ENZYMES ( 118 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BseAI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BsrI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HpaI KpnI Ksp632I
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NcoI
NdeI NruI NsiI NspI NspV
PacI PmeI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I TspRI XbaI
XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 945 GACGTC GACGT^C
AflIII 1151 ACACGT A^C[GA][CT]GT
AgeI 1328 ACCGGT A^CCGGT
AscI 955 GGCGCGCC GG^CGCGCC
AspI 849 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 667 CACGTG CAC^GTG
BfaI 448 CTAG C^TAG
BlpI 885 GCTGAGC GC^T[ACGT]AGC
BpmI 787 CTGGAG CTGGAG(16/14)
Bpu1102I 885 GCTGAGC GC^T[ACGT]AGC
BsaAI 667 CACGTG [CT]AC^GT[GA]
BsaWI 1328 ACCGGT [AT]^CCGG[AT]
BsiWI 305 CGTACG C^GTACG
BsmBI 947 CGTCTC CGTCTC(1/5)
CelII 885 GCTGAGC GC^T[ACGT]AGC
DdeI 886 CTGAG C^T[ACGT]AG
EcoT14I 428 CCAAGG C^C[AT][AT]GG
HindIII 718 AAGCTT A^AGCTT
MaeI 448 CTAG C^TAG
MaeIII 847 GTGAC ^GT[ACGT]AC
NheI 447 GCTAGC G^CTAGC
PaeR7I 1315 CTCGAG C^TCGAG
PflFI 849 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 980 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1328 ACCGGT A^CCGGT
PmaCI 667 CACGTG CAC^GTG
PmlI 667 CACGTG CAC^GTG
PpuMI 737 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 601 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 200 AGTACT AGT^ACT
SexAI 487 ACCTGGT A^CC[AT]GGT
SfaNI 1363 GATGC GCATC(5/9)
SgrAI 505 CGCCGGCG C[GA]^CCGG[CT]G
StuI 389 AGGCCT AGG^CCT
StyI 428 CCAAGG C^C[AT][AT]GG
TaqI 1316 TCGA T^CGA
TfiI 751 GAATC G^A[AT]TC
Tsp45I 847 GTGAC ^GT[GC]AC
Tth111I 849 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1316 TCGA T^CGA
Van91I 980 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1315 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwI 656 GGATC GGATC(4/5)
684 GGATC
ApaI 539 GGGCCC GGGCC^C
1035 GGGCCC
AspHI 403 GAGCAC G[AT]GC[AT]^C
693 GTGCTC
BseRI 100 GAGGAG GAGGAG(10/8)
811 GAGGAG
BsiHKAI 403 GAGCAC G[AT]GC[AT]^C
693 GTGCTC
BsrBI 1312 CCGCTC CCGCTC(-3/-3)
820 GAGCGG
BssSI 54 CTCGTG CACGAG(-5/-1)
690 CTCGTG
HphI 458 TCACC GGTGA(8/7)
473 TCACC
MboII 438 GAAGA GAAGA(8/7)
559 GAAGA
MslI 273 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
459 CACCAAGATG
NotI 1066 GCGGCCGC GC^GGCCGC
1237 GCGGCCGC
PstI 196 CTGCAG CTGCA^G
1204 CTGCAG
SfcI 196 CTGCAG C^T[GA][CT]AG
1204 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BsaHI
BsaI BsaJI BsgI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoNI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI KasI KspI MaeII MboI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PleI RsaI
SacII Sau3AI Sau96I ScrFI SfoI
SmaI SmlI TseI XmaCI XmaI