Restriction map for
pFN21AE4222
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
Asp718I AspI AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspEI BspLU11I BsrDI
BsrGI BssSI BstAPI BstXI BstYI
CpoI DraIII DrdI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HpaI KpnI MamI MfeI MflI
MluI MroI MunI NcoI NheI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI SexAI SnaBI SpeI
SphI Sse8387I SspBI StyI Tth111I
XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AhdI 1900 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2131 GGATC GGATC(4/5)
AocI 835 CCTGAGG CC^T[ACGT]AGG
Aor51HI 137 AGCGCT AGC^GCT
AspEI 1900 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1166 GTGCTC G[AT]GC[AT]^C
BalI 414 TGGCCA TGG^CCA
BlpI 914 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 914 GCTTAGC GC^T[ACGT]AGC
BsaWI 1896 ACCGGA [AT]^CCGG[AT]
BseRI 358 CTCCTC GAGGAG(10/8)
BsgI 664 CTGCAC GTGCAG(16/14)
BsiHKAI 1166 GTGCTC G[AT]GC[AT]^C
BspDI 1414 ATCGAT AT^CGAT
BspHI 2049 TCATGA T^CATGA
Bst1107I 1793 GTATAC GTA^TAC
BstEII 399 GGTGACC G^GT[ACGT]ACC
BstF5I 1305 GGATG GGATG(2/0)
BstPI 399 GGTGACC G^GT[ACGT]ACC
BstZ17I 1793 GTATAC GTA^TAC
Bsu36I 835 CCTGAGG CC^T[ACGT]AGG
CelII 914 GCTTAGC GC^T[ACGT]AGC
ClaI 1414 ATCGAT AT^CGAT
Eam1105I 1900 GACGGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1075 GAAGAG CTCTTC(1/4)
Eco47III 137 AGCGCT AGC^GCT
Eco81I 835 CCTGAGG CC^T[ACGT]AGG
EcoNI 1144 CCTGTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 399 GGTGACC G^GT[ACGT]ACC
FokI 1305 GGATG GGATG(9/13)
HgaI 581 GCGTC GACGC(5/10)
HincII 2268 GTTGAC GT[CT]^[GA]AC
HindII 2268 GTTGAC GT[CT]^[GA]AC
Ksp632I 1075 GAAGAG CTCTTC(1/4)
MaeII 1883 ACGT A^CGT
MluNI 414 TGGCCA TGG^CCA
MscI 414 TGGCCA TGG^CCA
MslI 1028 CATTACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 761 CATATG CA^TATG
NspI 1820 GCATGT [GA]CATG^[CT]
PpuMI 22 AGGACCT [GA]G^G[AT]CC[CT]
PstI 2073 CTGCAG CTGCA^G
RcaI 2049 TCATGA T^CATGA
ScaI 861 AGTACT AGT^ACT
SfiI 633 GGCCCGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 348 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 635 CCCGGG CCC^GGG
SspI 1707 AATATT AAT^ATT
StuI 1002 AGGCCT AGG^CCT
SwaI 800 ATTTAAAT ATTT^AAAT
XbaI 2302 TCTAGA T^CTAGA
XmaCI 635 CCCGGG C^CCGGG
XmaI 635 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 259 GGCGCC G[GA]^CG[CT]C
346 GGCGCC
ApaI 233 GGGCCC GGGCC^C
632 GGGCCC
AseI 1410 ATTAAT AT^TAAT
1611 ATTAAT
AsnI 1410 ATTAAT AT^TAAT
1611 ATTAAT
Asp700I 1240 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2152 GAAAACCTTC
BanI 259 GGCGCC G^G[CT][GA]CC
346 GGCGCC
BbeI 259 GGCGCC GGCGC^C
346 GGCGCC
BglI 634 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
700 GCCTGCGAGGC
BsaHI 259 GGCGCC G[GA]^CG[CT]C
346 GGCGCC
BsaI 2306 GAGACC GGTCTC(1/5)
769 GGTCTC
BsmFI 476 GGGAC GGGAC(10/14)
520 GGGAC
BspMI 939 ACCTGC ACCTGC(4/8)
2178 ACCTGC
BsrBI 628 GAGCGG CCGCTC(-3/-3)
752 GAGCGG
BssHII 117 GCGCGC G^CGCGC
197 GCGCGC
BstBI 1784 TTCGAA TT^CGAA
2105 TTCGAA
DpnII 1522 GATC ^GATC
2132 GATC
Hin1I 259 GGCGCC G[GA]^CG[CT]C
346 GGCGCC
HindIII 1155 AAGCTT A^AGCTT
1554 AAGCTT
KasI 259 GGCGCC G^GCGCC
346 GGCGCC
MboI 1522 GATC ^GATC
2132 GATC
NaeI 349 GCCGGC GCC^GGC
643 GCCGGC
NarI 259 GGCGCC GG^CGCC
346 GGCGCC
NdeII 1522 GATC ^GATC
2132 GATC
NgoMIV 349 GCCGGC G^CCGGC
643 GCCGGC
NotI 121 GCGGCCGC GC^GGCCGC
531 GCGGCCGC
NspV 1784 TTCGAA TT^CGAA
2105 TTCGAA
PflMI 105 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
300 CCACGGGCTGG
PshBI 1410 ATTAAT AT^TAAT
1611 ATTAAT
Sau3AI 1522 GATC ^GATC
2132 GATC
SfcI 2073 CTGCAG C^T[GA][CT]AG
2213 CTGTAG
SfoI 259 GGCGCC GGC^GCC
346 GGCGCC
SfuI 1784 TTCGAA TT^CGAA
2105 TTCGAA
SmlI 1507 CTTGAG C^T[CT][GA]AG
2066 CTCAAG
Van91I 105 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
300 CCACGGGCTGG
XmnI 1240 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2152 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwNI ApoI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiEI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeIII MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI PvuII RsaI
SacII Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I