Restriction map for
pFN21AE4167
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwNI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbsI
BbvCI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI HincII
HindII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 1618 GGCGCC G[GA]^CG[CT]C
AocI 244 CCTGAGG CC^T[ACGT]AGG
AspI 232 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1575 CCCGGG C^[CT]CG[GA]G
AviII 1316 TGCGCA TGC^GCA
BbeI 1618 GGCGCC GGCGC^C
BbrPI 1828 CACGTG CAC^GTG
BfaI 202 CTAG C^TAG
BsaAI 1828 CACGTG [CT]AC^GT[GA]
BsaHI 1618 GGCGCC G[GA]^CG[CT]C
BsaI 732 GAGACC GGTCTC(1/5)
BsiEI 1437 CGGCCG CG[GA][CT]^CG
BsmI 29 GCATTC GAATGC(1/-1)
BsoBI 1575 CCCGGG C^[CT]CG[GA]G
BsrDI 305 GCAATG GCAATG(2/0)
Bsu36I 244 CCTGAGG CC^T[ACGT]AGG
EagI 1437 CGGCCG C^GGCCG
EclXI 1437 CGGCCG C^GGCCG
Eco52I 1437 CGGCCG C^GGCCG
Eco81I 244 CCTGAGG CC^T[ACGT]AGG
FseI 1797 GGCCGGCC GGCCGG^CC
FspI 1316 TGCGCA TGC^GCA
HgaI 1433 GACGC GACGC(5/10)
Hin1I 1618 GGCGCC G[GA]^CG[CT]C
HindIII 181 AAGCTT A^AGCTT
KasI 1618 GGCGCC G^GCGCC
KspI 1138 CCGCGG CCGC^GG
MaeI 202 CTAG C^TAG
MaeIII 101 GTGAC ^GT[ACGT]AC
NarI 1618 GGCGCC GG^CGCC
NcoI 821 CCATGG C^CATGG
NotI 1436 GCGGCCGC GC^GGCCGC
PflFI 232 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 625 GACTC GAGTC(4/5)
PmaCI 1828 CACGTG CAC^GTG
PmlI 1828 CACGTG CAC^GTG
PstI 162 CTGCAG CTGCA^G
SacI 19 GAGCTC GAGCT^C
SacII 1138 CCGCGG CCGC^GG
ScaI 170 AGTACT AGT^ACT
SfcI 162 CTGCAG C^T[GA][CT]AG
SfiI 1792 GGCCTGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1618 GGCGCC GGC^GCC
SmaI 1575 CCCGGG CCC^GGG
SmlI 1303 CTCAAG C^T[CT][GA]AG
Tsp45I 101 GTGAC ^GT[GC]AC
Tth111I 232 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1592 CCAAGCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1575 CCCGGG C^CCGGG
XmaI 1575 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 171 GTAC GT^AC
705 GTAC
Alw44I 1179 GTGCAC G^TGCAC
1288 GTGCAC
ApaI 1480 GGGCCC GGGCC^C
1578 GGGCCC
ApaLI 1179 GTGCAC G^TGCAC
1288 GTGCAC
Asp700I 180 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1668 GAAGCTGTTC
BcgI 987 CGACTACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
126 GCAGGAGTATCG
BspLU11I 935 ACATGT A^CATGT
1175 ACATGT
BspMI 947 ACCTGC ACCTGC(4/8)
1385 ACCTGC
BssHII 1680 GCGCGC G^CGCGC
1682 GCGCGC
BstAPI 1181 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1265 GCATCCACTGC
BstYI 344 AGATCC [GA]^GATC[CT]
840 GGATCT
DsaI 821 CCATGG C^C[GA][CT]GG
1138 CCGCGG
MflI 344 AGATCC [GA]^GATC[CT]
840 GGATCT
RsaI 171 GTAC GT^AC
705 GTAC
SapI 1060 GAAGAGC GCTCTTC(1/4)
1473 GCTCTTC
TfiI 682 GAATC G^A[AT]TC
855 GAATC
XhoII 344 AGATCC [GA]^GATC[CT]
840 GGATCT
XmnI 180 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1668 GAAGCTGTTC
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AflIII AluI AlwI
AspHI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PpuMI PvuII Sau3AI Sau96I
ScrFI SfaNI StuI StyI TaqI
TseI TspRI TthHB8I