Restriction map for
pFN21AE1260
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII BbsI BbvCI
BciVI BclI BfrI BglII BpuAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp509I Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Alw44I 1476 GTGCAC G^TGCAC
AlwNI 1401 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1476 GTGCAC G^TGCAC
Asp700I 1032 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1476 GTGCAC G[AT]GC[AT]^C
AvaI 433 CCCGGG C^[CT]CG[GA]G
AvrII 1063 CCTAGG C^CTAGG
BalI 534 TGGCCA TGG^CCA
BcgI 1220 GCAGCAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1063 CCTAGG C^CTAGG
BpmI 1655 CTGGAG CTGGAG(16/14)
BsiHKAI 1476 GTGCAC G[AT]GC[AT]^C
BsoBI 433 CCCGGG C^[CT]CG[GA]G
BspLU11I 599 ACATGT A^CATGT
BsrDI 1134 CATTGC GCAATG(2/0)
BstAPI 648 GCACTCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 725 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 725 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 1057 GATATC GAT^ATC
HgaI 1195 GACGC GACGC(5/10)
HphI 1445 TCACC GGTGA(8/7)
MluNI 534 TGGCCA TGG^CCA
MscI 534 TGGCCA TGG^CCA
MslI 1567 CACTCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 735 TCGCGA TCG^CGA
NspI 599 ACATGT [GA]CATG^[CT]
PpuMI 725 GGGACCT [GA]G^G[AT]CC[CT]
ScaI 757 AGTACT AGT^ACT
SmaI 433 CCCGGG CCC^GGG
SmlI 103 CTCAAG C^T[CT][GA]AG
XmaCI 433 CCCGGG C^CCGGG
XmaI 433 CCCGGG C^CCGGG
XmnI 1032 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 599 ACATGT A^C[GA][CT]GT
1472 ACGTGT
Aor51HI 679 AGCGCT AGC^GCT
1247 AGCGCT
ApaI 436 GGGCCC GGGCC^C
1309 GGGCCC
BbrPI 1471 CACGTG CAC^GTG
1555 CACGTG
BglI 307 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
711 GCCGGAGTGGC
BlpI 184 GCTGAGC GC^T[ACGT]AGC
1713 GCTGAGC
Bpu1102I 184 GCTGAGC GC^T[ACGT]AGC
1713 GCTGAGC
BsaAI 1471 CACGTG [CT]AC^GT[GA]
1555 CACGTG
BsiEI 296 CGGCCG CG[GA][CT]^CG
686 CGGCCG
BspMI 56 ACCTGC ACCTGC(4/8)
728 ACCTGC
BsrBI 100 CCGCTC CCGCTC(-3/-3)
559 CCGCTC
BssHII 134 GCGCGC G^CGCGC
180 GCGCGC
CelII 184 GCTGAGC GC^T[ACGT]AGC
1713 GCTGAGC
DsaI 119 CCGCGG C^C[GA][CT]GG
211 CCGCGG
EagI 296 CGGCCG C^GGCCG
686 CGGCCG
EclXI 296 CGGCCG C^GGCCG
686 CGGCCG
Eco47III 679 AGCGCT AGC^GCT
1247 AGCGCT
Eco52I 296 CGGCCG C^GGCCG
686 CGGCCG
EcoNI 82 CCTCACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
826 CCTTTCCAAGG
EcoT22I 634 ATGCAT ATGCA^T
1131 ATGCAT
KspI 119 CCGCGG CCGC^GG
211 CCGCGG
NaeI 246 GCCGGC GCC^GGC
659 GCCGGC
NgoMIV 246 GCCGGC G^CCGGC
659 GCCGGC
NotI 295 GCGGCCGC GC^GGCCGC
685 GCGGCCGC
NsiI 634 ATGCAT ATGCA^T
1131 ATGCAT
PleI 1269 GACTC GAGTC(4/5)
1355 GAGTC
PmaCI 1471 CACGTG CAC^GTG
1555 CACGTG
PmlI 1471 CACGTG CAC^GTG
1555 CACGTG
PvuII 343 CAGCTG CAG^CTG
1711 CAGCTG
SacII 119 CCGCGG CCGC^GG
211 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BamHI BanI BanII
BbeI BbvI BcnI BfaI BmyI
BsaHI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII