Restriction map for
pFN21AE4094
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII Hin1I HpaI HphI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tru9I Van91I XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 741 GTCTAC GT^[AC][GT]AC
AccII 8 CGCG CG^CG
AclI 1087 AACGTT AA^CGTT
Alw44I 1127 GTGCAC G^TGCAC
ApaLI 1127 GTGCAC G^TGCAC
AspHI 1127 GTGCAC G[AT]GC[AT]^C
AspI 1001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1245 TGGCCA TGG^CCA
BanII 556 GAGCCC G[GA]GC[CT]^C
BbsI 320 GAAGAC GAAGAC(2/6)
BlpI 1159 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1159 GCTCAGC GC^T[ACGT]AGC
BpuAI 320 GAAGAC GAAGAC(2/6)
BseRI 79 GAGGAG GAGGAG(10/8)
BsgI 719 CTGCAC GTGCAG(16/14)
BsiHKAI 1127 GTGCAC G[AT]GC[AT]^C
BspDI 948 ATCGAT AT^CGAT
BsrFI 468 GCCGGC [GA]^CCGG[CT]
BstAPI 922 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 317 TTCGAA TT^CGAA
BstUI 8 CGCG CG^CG
CelII 1159 GCTCAGC GC^T[ACGT]AGC
Cfr10I 468 GCCGGC [GA]^CCGG[CT]
ClaI 948 ATCGAT AT^CGAT
EaeI 1245 TGGCCA [CT]^GGCC[GA]
EarI 436 GAAGAG CTCTTC(1/4)
EcoNI 1231 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 173 GAATTC G^AATTC
EcoT14I 1101 CCAAGG C^C[AT][AT]GG
HapII 469 CCGG C^CGG
HgaI 6 GACGC GACGC(5/10)
HincII 217 GTCAAC GT[CT]^[GA]AC
HindII 217 GTCAAC GT[CT]^[GA]AC
HindIII 67 AAGCTT A^AGCTT
HpaII 469 CCGG C^CGG
Ksp632I 436 GAAGAG CTCTTC(1/4)
MaeII 1088 ACGT A^CGT
MluNI 1245 TGGCCA TGG^CCA
MscI 1245 TGGCCA TGG^CCA
MspI 469 CCGG C^CGG
MvnI 8 CGCG CG^CG
NaeI 468 GCCGGC GCC^GGC
NgoMIV 468 GCCGGC G^CCGGC
NspV 317 TTCGAA TT^CGAA
PflFI 1001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1087 AACGTT AA^CGTT
SfuI 317 TTCGAA TT^CGAA
SspI 139 AATATT AAT^ATT
StyI 1101 CCAAGG C^C[AT][AT]GG
TfiI 1191 GAATC G^A[AT]TC
Tsp45I 604 GTGAC ^GT[GC]AC
Tth111I 1001 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 762 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 1153 CCGC CCGC(-3/-1)
553 GCGG
AcsI 173 GAATTC [GA]^AATT[CT]
346 GAATTT
AlwI 567 GGATC GGATC(4/5)
759 GGATC
ApoI 173 GAATTC [GA]^AATT[CT]
346 GAATTT
BanI 597 GGCACC G^G[CT][GA]CC
624 GGTGCC
BcgI 387 CGATAAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
950 CGATGGGCGTGC
BfaI 223 CTAG C^TAG
763 CTAG
BmyI 556 GAGCCC G[AGT]GC[ACT]^C
1127 GTGCAC
BpmI 1254 CTCCAG CTGGAG(16/14)
517 CTGGAG
BsaJI 203 CCAGGG C^C[ACGT][ACGT]GG
1101 CCAAGG
Bsp1286I 556 GAGCCC G[AGT]GC[ACT]^C
1127 GTGCAC
BspMI 681 ACCTGC ACCTGC(4/8)
1237 GCAGGT
BstF5I 57 CATCC GGATG(2/0)
1270 GGATG
BstYI 567 GGATCT [GA]^GATC[CT]
759 GGATCT
CfoI 9 GCGC GCG^C
98 GCGC
DraII 117 AGGTCCT [GA]G^G[ACGT]CC[CT]
891 GGGACCC
EcoO109I 117 AGGTCCT [GA]G^G[ACGT]CC[CT]
891 GGGACCC
FokI 57 CATCC GGATG(9/13)
1270 GGATG
HhaI 9 GCGC GCG^C
98 GCGC
HinP1I 9 GCGC G^CGC
98 GCGC
MaeI 223 CTAG C^TAG
763 CTAG
MaeIII 604 GTGAC ^GT[ACGT]AC
748 GTAAC
MflI 567 GGATCT [GA]^GATC[CT]
759 GGATCT
NspI 254 GCATGC [GA]CATG^[CT]
1025 GCATGC
PpuMI 117 AGGTCCT [GA]G^G[AT]CC[CT]
891 GGGACCC
ScaI 92 AGTACT AGT^ACT
871 AGTACT
SfaNI 1071 GATGC GCATC(5/9)
158 GCATC
SphI 254 GCATGC GCATG^C
1025 GCATGC
Tsp509I 174 AATT ^AATT
347 AATT
XcmI 1037 CCAATGGGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1134 CCAGAGTAAGTTTGG
XhoII 567 GGATCT [GA]^GATC[CT]
759 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI AlwNI AvaII BbvI
BsiYI BslI BsmFI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII HinfI ItaI
MboI MboII MnlI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI TaqI TseI
TspRI TthHB8I