Restriction map for
pFN21AE4323
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FbaI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tru9I Tsp509I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 305 CGCG CG^CG
AfaI 820 GTAC GT^AC
AspI 75 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 832 GGATCC G^GATCC
BlpI 36 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
BsaI 728 GAGACC GGTCTC(1/5)
BseRI 99 CTCCTC GAGGAG(10/8)
BsmAI 728 GAGAC GTCTC(1/5)
Bsp1407I 819 TGTACA T^GTACA
BsrBI 168 CCGCTC CCGCTC(-3/-3)
BsrFI 610 GCCGGC [GA]^CCGG[CT]
BsrGI 819 TGTACA T^GTACA
BstEII 147 GGTCACC G^GT[ACGT]ACC
BstPI 147 GGTCACC G^GT[ACGT]ACC
BstUI 305 CGCG CG^CG
BstXI 176 CCAGCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 36 GCTGAGC GC^T[ACGT]AGC
Cfr10I 610 GCCGGC [GA]^CCGG[CT]
DrdI 883 GACTCTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 725 GAAGAG CTCTTC(1/4)
EcoO65I 147 GGTCACC G^GT[ACGT]ACC
FseI 609 GGCCGGCC GGCCGG^CC
HaeII 347 GGCGCT [GA]GCGC^[CT]
HgaI 779 GCGTC GACGC(5/10)
Ksp632I 725 GAAGAG CTCTTC(1/4)
MaeII 47 ACGT A^CGT
MvnI 305 CGCG CG^CG
NaeI 610 GCCGGC GCC^GGC
NgoMIV 610 GCCGGC G^CCGGC
PflFI 75 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 195 CAGCTG CAG^CTG
RsaI 820 GTAC GT^AC
SfiI 604 GGCCTGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 522 CCCGGG CCC^GGG
SspBI 819 TGTACA T^GTACA
StuI 603 AGGCCT AGG^CCT
Tth111I 75 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 522 CCCGGG C^CCGGG
XmaI 522 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 777 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
823 CAGTCCCTG
ApaI 249 GGGCCC GGGCC^C
787 GGGCCC
AvaI 102 CTCGGG C^[CT]CG[GA]G
522 CCCGGG
BanI 207 GGTGCC G^G[CT][GA]CC
709 GGCACC
BbsI 508 GAAGAC GAAGAC(2/6)
892 GTCTTC
BfaI 181 CTAG C^TAG
503 CTAG
BpuAI 508 GAAGAC GAAGAC(2/6)
892 GTCTTC
BsoBI 102 CTCGGG C^[CT]CG[GA]G
522 CCCGGG
BsrI 372 CCAGT ACTGG(1/-1)
739 CCAGT
BstF5I 59 CATCC GGATG(2/0)
492 CATCC
BstYI 3 GGATCT [GA]^GATC[CT]
832 GGATCC
DpnII 4 GATC ^GATC
833 GATC
EcoNI 180 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
755 CCTGCCGAAGG
FokI 59 CATCC GGATG(9/13)
492 CATCC
MaeI 181 CTAG C^TAG
503 CTAG
MboI 4 GATC ^GATC
833 GATC
MflI 3 GGATCT [GA]^GATC[CT]
832 GGATCC
NcoI 66 CCATGG C^CATGG
381 CCATGG
NdeII 4 GATC ^GATC
833 GATC
NlaIII 67 CATG CATG^
382 CATG
PpuMI 105 GGGACCC [GA]G^G[AT]CC[CT]
668 GGGACCT
Sau3AI 4 GATC ^GATC
833 GATC
SfaNI 551 GCATC GCATC(5/9)
962 GCATC
TaqI 723 TCGA T^CGA
896 TCGA
TspRI 282 CACTG CA[GC]TG[ACGT][ACGT]^
677 CACTG
TthHB8I 723 TCGA T^CGA
896 TCGA
XcmI 173 CCACCAGCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
372 CCAGTTTGCCCATGG
XhoII 3 GGATCT [GA]^GATC[CT]
832 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIV PflMI PleI Sau96I ScrFI
StyI TseI Tsp45I Van91I