Restriction map for
pFN21AB7678
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccI AccIII AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BsrDI BssHII Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FokI FseI
FspI HgaI HincII HindII HindIII
HpaI HphI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NdeI NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tru9I Tth111I XbaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 178 AACGTT AA^CGTT
AcsI 528 GAATTT [GA]^AATT[CT]
ApaI 1178 GGGCCC GGGCC^C
ApoI 528 GAATTT [GA]^AATT[CT]
Asp700I 465 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 687 GGATCC G^GATCC
BanII 1178 GGGCCC G[GA]GC[CT]^C
BbsI 538 GAAGAC GAAGAC(2/6)
BbvCI 388 GCTGAGG CCTCAGC(-5/-2)
BmyI 1178 GGGCCC G[AGT]GC[ACT]^C
BpuAI 538 GAAGAC GAAGAC(2/6)
BsgI 547 GTGCAG GTGCAG(16/14)
BsiEI 347 CGACCG CG[GA][CT]^CG
Bsp1286I 1178 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 1164 TGTACA T^GTACA
BspMI 549 GCAGGT ACCTGC(4/8)
BsrFI 366 GCCGGC [GA]^CCGG[CT]
BsrGI 1164 TGTACA T^GTACA
BsrI 1150 ACTGG ACTGG(1/-1)
BssSI 238 CACGAG CACGAG(-5/-1)
BstYI 687 GGATCC [GA]^GATC[CT]
Cfr10I 366 GCCGGC [GA]^CCGG[CT]
DpnII 688 GATC ^GATC
EcoT22I 1202 ATGCAT ATGCA^T
FauI 14 GCGGG CCCGC(4/6)
MboI 688 GATC ^GATC
MflI 687 GGATCC [GA]^GATC[CT]
NaeI 366 GCCGGC GCC^GGC
NdeII 688 GATC ^GATC
NgoMIV 366 GCCGGC G^CCGGC
NsiI 1202 ATGCAT ATGCA^T
PaeR7I 310 CTCGAG C^TCGAG
PflMI 636 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 4 GAGTC GAGTC(4/5)
Psp1406I 178 AACGTT AA^CGTT
PstI 451 CTGCAG CTGCA^G
PvuII 448 CAGCTG CAG^CTG
SapI 569 GAAGAGC GCTCTTC(1/4)
Sau3AI 688 GATC ^GATC
SfcI 451 CTGCAG C^T[GA][CT]AG
SmlI 310 CTCGAG C^T[CT][GA]AG
SspBI 1164 TGTACA T^GTACA
TfiI 478 GAATC G^A[AT]TC
Tsp509I 529 AATT ^AATT
Van91I 636 CCACCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 310 CTCGAG C^TCGAG
XhoII 687 GGATCC [GA]^GATC[CT]
XmnI 465 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 647 ACATGT A^C[GA][CT]GT
1137 ACATGT
AlwI 688 GATCC GGATC(4/5)
687 GGATC
BfaI 460 CTAG C^TAG
725 CTAG
BsmAI 510 GAGAC GTCTC(1/5)
6 GTCTC
BspLU11I 647 ACATGT A^CATGT
1137 ACATGT
Cac8I 366 GCCGGC GC[ACGT]^[ACGT]GC
520 GCACGC
DdeI 152 CTGAG C^T[ACGT]AG
389 CTGAG
DraII 661 GGGGCCT [GA]G^G[ACGT]CC[CT]
1178 GGGCCCC
EarI 8 CTCTTC CTCTTC(1/4)
569 GAAGAG
EcoO109I 661 GGGGCCT [GA]G^G[ACGT]CC[CT]
1178 GGGCCCC
HinfI 4 GAGTC G^A[ACGT]TC
478 GAATC
Ksp632I 8 CTCTTC CTCTTC(1/4)
569 GAAGAG
MaeI 460 CTAG C^TAG
725 CTAG
MaeII 179 ACGT A^CGT
494 ACGT
MaeIII 97 GTGAC ^GT[ACGT]AC
1134 GTCAC
MspA1I 386 CCGCTG C[AC]G^C[GT]G
448 CAGCTG
Tsp45I 97 GTGAC ^GT[GC]AC
1134 GTCAC
XcmI 24 CCACAGTGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
761 CCAGGCCACCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BbeI BbvI
BcnI BglI BsaHI BsaJI BseRI
BsiYI BslI BsmFI BsoBI BssKI
BstNI BstUI CfoI Cfr13I DsaI
EaeI EcoRII EcoT14I Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI KasI MboII MnlI
MspI MvaI MvnI MwoI NarI
NciI NcoI NlaIII NlaIV NspI
RsaI Sau96I ScrFI SfoI SmaI
StyI TaqI TseI TspRI TthHB8I
XmaCI XmaI