################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BsaAI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I HindIII KpnI KspI MamI MfeI MluI MluNI MscI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 607 GTCTAC GT^[AC][GT]AC AccIII 800 TCCGGA T^CCGGA AgeI 70 ACCGGT A^CCGGT ApaI 1356 GGGCCC GGGCC^C BclI 773 TGATCA T^GATCA BlpI 934 GCTAAGC GC^T[ACGT]AGC Bpu1102I 934 GCTAAGC GC^T[ACGT]AGC BseAI 800 TCCGGA T^CCGGA BsgI 599 CTGCAC GTGCAG(16/14) BsmBI 5 CGTCTC CGTCTC(1/5) BspEI 800 TCCGGA T^CCGGA BspLU11I 1064 ACATGT A^CATGT BsrBI 628 GAGCGG CCGCTC(-3/-3) BssSI 661 CACGAG CACGAG(-5/-1) BstXI 716 CCAAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 934 GCTAAGC GC^T[ACGT]AGC DraI 1362 TTTAAA TTT^AAA DsaI 1007 CCATGG C^C[GA][CT]GG FbaI 773 TGATCA T^GATCA FseI 218 GGCCGGCC GGCCGG^CC HgaI 122 GACGC GACGC(5/10) HincII 1060 GTTAAC GT[CT]^[GA]AC HindII 1060 GTTAAC GT[CT]^[GA]AC HpaI 1060 GTTAAC GTT^AAC MroI 800 TCCGGA T^CCGGA MspA1I 482 CCGCTG C[AC]G^C[GT]G NcoI 1007 CCATGG C^CATGG NspI 1064 ACATGT [GA]CATG^[CT] PaeR7I 649 CTCGAG C^TCGAG PinAI 70 ACCGGT A^CCGGT PpuMI 449 GGGACCT [GA]G^G[AT]CC[CT] SacI 868 GAGCTC GAGCT^C SgrAI 69 CACCGGTG C[GA]^CCGG[CT]G SmaI 209 CCCGGG CCC^GGG SmlI 649 CTCGAG C^T[CT][GA]AG XhoI 649 CTCGAG C^TCGAG XmaCI 209 CCCGGG C^CCGGG XmaI 209 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 689 ACACGT A^C[GA][CT]GT 1064 ACATGT AlwI 1601 GATCC GGATC(4/5) 726 GGATC AspHI 16 GTGCTC G[AT]GC[AT]^C 868 GAGCTC AvaI 209 CCCGGG C^[CT]CG[GA]G 649 CTCGAG AviII 239 TGCGCA TGC^GCA 743 TGCGCA BbeI 373 GGCGCC GGCGC^C 531 GGCGCC BbsI 733 GAAGAC GAAGAC(2/6) 667 GTCTTC BfaI 1432 CTAG C^TAG 1474 CTAG BpuAI 733 GAAGAC GAAGAC(2/6) 667 GTCTTC BsaI 712 GAGACC GGTCTC(1/5) 1229 GAGACC BsaWI 70 ACCGGT [AT]^CCGG[AT] 800 TCCGGA BsiEI 217 CGGCCG CG[GA][CT]^CG 1333 CGGCCG BsiHKAI 16 GTGCTC G[AT]GC[AT]^C 868 GAGCTC BsoBI 209 CCCGGG C^[CT]CG[GA]G 649 CTCGAG BspMI 452 ACCTGC ACCTGC(4/8) 1206 GCAGGT BstYI 726 GGATCT [GA]^GATC[CT] 1600 AGATCC EagI 217 CGGCCG C^GGCCG 1333 CGGCCG EclXI 217 CGGCCG C^GGCCG 1333 CGGCCG Eco52I 217 CGGCCG C^GGCCG 1333 CGGCCG FspI 239 TGCGCA TGC^GCA 743 TGCGCA KasI 373 GGCGCC G^GCGCC 531 GGCGCC MaeI 1432 CTAG C^TAG 1474 CTAG MflI 726 GGATCT [GA]^GATC[CT] 1600 AGATCC MseI 1061 TTAA T^TAA 1363 TTAA NaeI 61 GCCGGC GCC^GGC 219 GCCGGC NarI 373 GGCGCC GG^CGCC 531 GGCGCC NgoMIV 61 GCCGGC G^CCGGC 219 GCCGGC PleI 187 GACTC GAGTC(4/5) 502 GACTC ScaI 509 AGTACT AGT^ACT 1122 AGTACT SfcI 609 CTACAG C^T[GA][CT]AG 699 CTACAG SfoI 373 GGCGCC GGC^GCC 531 GGCGCC Tru9I 1061 TTAA T^TAA 1363 TTAA XhoII 726 GGATCT [GA]^GATC[CT] 1600 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI SapI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI