Restriction map for
pFN21AE3232
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BsaAI BsaBI BsiWI BsmI Bsp1407I
BspDI BspHI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I HindIII
KpnI KspI MamI MfeI MluI
MluNI MscI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 607 GTCTAC GT^[AC][GT]AC
AccIII 800 TCCGGA T^CCGGA
AgeI 70 ACCGGT A^CCGGT
ApaI 1356 GGGCCC GGGCC^C
BclI 773 TGATCA T^GATCA
BlpI 934 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 934 GCTAAGC GC^T[ACGT]AGC
BseAI 800 TCCGGA T^CCGGA
BsgI 599 CTGCAC GTGCAG(16/14)
BsmBI 5 CGTCTC CGTCTC(1/5)
BspEI 800 TCCGGA T^CCGGA
BspLU11I 1064 ACATGT A^CATGT
BsrBI 628 GAGCGG CCGCTC(-3/-3)
BssSI 661 CACGAG CACGAG(-5/-1)
BstXI 716 CCAAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 934 GCTAAGC GC^T[ACGT]AGC
DraI 1362 TTTAAA TTT^AAA
DsaI 1007 CCATGG C^C[GA][CT]GG
FbaI 773 TGATCA T^GATCA
FseI 218 GGCCGGCC GGCCGG^CC
HgaI 122 GACGC GACGC(5/10)
HincII 1060 GTTAAC GT[CT]^[GA]AC
HindII 1060 GTTAAC GT[CT]^[GA]AC
HpaI 1060 GTTAAC GTT^AAC
MroI 800 TCCGGA T^CCGGA
MspA1I 482 CCGCTG C[AC]G^C[GT]G
NcoI 1007 CCATGG C^CATGG
NspI 1064 ACATGT [GA]CATG^[CT]
PaeR7I 649 CTCGAG C^TCGAG
PinAI 70 ACCGGT A^CCGGT
PpuMI 449 GGGACCT [GA]G^G[AT]CC[CT]
SacI 868 GAGCTC GAGCT^C
SgrAI 69 CACCGGTG C[GA]^CCGG[CT]G
SmaI 209 CCCGGG CCC^GGG
SmlI 649 CTCGAG C^T[CT][GA]AG
XhoI 649 CTCGAG C^TCGAG
XmaCI 209 CCCGGG C^CCGGG
XmaI 209 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 689 ACACGT A^C[GA][CT]GT
1064 ACATGT
AlwI 1601 GATCC GGATC(4/5)
726 GGATC
AspHI 16 GTGCTC G[AT]GC[AT]^C
868 GAGCTC
AvaI 209 CCCGGG C^[CT]CG[GA]G
649 CTCGAG
AviII 239 TGCGCA TGC^GCA
743 TGCGCA
BbeI 373 GGCGCC GGCGC^C
531 GGCGCC
BbsI 733 GAAGAC GAAGAC(2/6)
667 GTCTTC
BfaI 1432 CTAG C^TAG
1474 CTAG
BpuAI 733 GAAGAC GAAGAC(2/6)
667 GTCTTC
BsaI 712 GAGACC GGTCTC(1/5)
1229 GAGACC
BsaWI 70 ACCGGT [AT]^CCGG[AT]
800 TCCGGA
BsiEI 217 CGGCCG CG[GA][CT]^CG
1333 CGGCCG
BsiHKAI 16 GTGCTC G[AT]GC[AT]^C
868 GAGCTC
BsoBI 209 CCCGGG C^[CT]CG[GA]G
649 CTCGAG
BspMI 452 ACCTGC ACCTGC(4/8)
1206 GCAGGT
BstYI 726 GGATCT [GA]^GATC[CT]
1600 AGATCC
EagI 217 CGGCCG C^GGCCG
1333 CGGCCG
EclXI 217 CGGCCG C^GGCCG
1333 CGGCCG
Eco52I 217 CGGCCG C^GGCCG
1333 CGGCCG
FspI 239 TGCGCA TGC^GCA
743 TGCGCA
KasI 373 GGCGCC G^GCGCC
531 GGCGCC
MaeI 1432 CTAG C^TAG
1474 CTAG
MflI 726 GGATCT [GA]^GATC[CT]
1600 AGATCC
MseI 1061 TTAA T^TAA
1363 TTAA
NaeI 61 GCCGGC GCC^GGC
219 GCCGGC
NarI 373 GGCGCC GG^CGCC
531 GGCGCC
NgoMIV 61 GCCGGC G^CCGGC
219 GCCGGC
PleI 187 GACTC GAGTC(4/5)
502 GACTC
ScaI 509 AGTACT AGT^ACT
1122 AGTACT
SfcI 609 CTACAG C^T[GA][CT]AG
699 CTACAG
SfoI 373 GGCGCC GGC^GCC
531 GGCGCC
Tru9I 1061 TTAA T^TAA
1363 TTAA
XhoII 726 GGATCT [GA]^GATC[CT]
1600 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI SapI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI